Pharmacological characterisation of extracts of coffee dusts.

Zuśkin, E; Duncan, Pamela G.; Douglas, James S.

doi:10.1136/oem.40.2.193

Cited by 11 publications

(5 citation statements)

References 16 publications

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“…The response may represent a direct irritant effect or possibly some pharmacologically related phenomenon. These data are similar to the results obtained with green coffee dust and spices on isolated guinea pig tracheal rings [Zuskin et al, 1983;1988bJ. The production of animal food is a complex process including many nutritional components as well as the addition of antibiotics and hormones. In addition, these foods are frequently contaminated with microorganisms [Vyskocil et al, 19721.…”

Section: Discussionsupporting

confidence: 94%

Immunological and respiratory changes in animal food processing workers

Zuśkin

Kanceljak

et al. 1992

American J Industrial Med

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A group of 35 men employed in the processing of animal food was studied to assess the relation between respiratory findings and immunological status. The most frequent positive skin prick reactions to occupational allergens were to fish flour (82.9%), followed by carotene (77.1%), corn (65.7%), four-leaf clover (62.9%), sunflower (54.3%), chicken meat (31.4%), soy (28.6%), and yeast (22.7%). Increased total IgE serum levels were found in 14/35 (40.0%) animal food workers compared to 1/39 (2.6%) in a healthy population (p less than 0.01). A significantly higher prevalence of chronic respiratory symptoms was found among the exposed workers compared to control workers. There was however, no significant difference in the prevalence of chronic respiratory symptoms between animal food workers with positive and negative skin tests to house dust or to fish flour or among those with increased or normal IgE (except for dyspnea). The frequency of acute symptoms associated with the work shift was high among the animal food workers but similar by immunological status. There were significant mean across-shift reductions for all ventilatory capacity tests, being particularly pronounced for FEF25. Workers with positive skin tests to fish flour antigen had significantly larger across-shift reductions in FEF25 than workers with negative skin reactions. An aqueous extract of animal food dust caused a dose-related contractile response of isolated guinea pig tracheal muscle in vitro. Our data suggest that, in addition to any immunological response animal food dust may produce in vivo, it probably also causes direct irritant or pharmacological reactions on the airways as suggested by our in vitro data.

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Section: Discussionsupporting

confidence: 94%

Immunological and respiratory changes in animal food processing workers

Zuśkin

Kanceljak

et al. 1992

American J Industrial Med

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“…The data obtained in our study are similar to those of our previous investigations of airway smooth muscles in the isolated guinea pig trachea with different organic dust extracts, such as coffee, soy, spices, animal food, and brewery dust (hops, barley, yeast) [11,12,14,[22][23][24]. These pharmacologic studies of latex and other extracts of organic dusts on isolated guinea pig tracheal smooth muscle suggest a complex nonimmunologic (non-IgE) effect of these airway irritants.…”

Section: Discussionsupporting

confidence: 80%

“…In a series of prior investigations [11][12][13][14][15] we have demonstrated that a number of organic dusts, e.g. coffee, soy, wool, brewery agents (hops, barley, yeast) and spices, cause nonspecific constriction of airway smooth muscle.…”

Section: Introductionmentioning

confidence: 99%

Pharmacologic Characterization of Latex Extracts by Isolated Guinea Pig Tracheal Tissue

Zuskin²,

Rienzi³

et al. 1998

Respiration

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Latex manufacturing workers are exposed to a heterogeneous aerosol of organic compounds. Previous studies of latex workers involved in glove production indicate that these individuals are at risk of developing respiratory symptoms and impaired lung function. The effect of latex extracts on isolated guinea pig tracheal smooth muscles was studied using latex water-soluble extracts obtained at different stages of the industrial process. Latex extracts were prepared as a 1:10 (w/v) solution. Dose-related contractions of nonsensitized guinea pig trachea were demonstrated using two latex extracts (latex 1 and latex 2). Latex 1 was prepared from the native latex and latex 2 from a processed form of latex which was relatively free of soluble proteins. Pharmacologic studies were performed by pretreating guinea pig tracheal tissue with drugs known to modulate smooth muscle contraction: atropine, indomethacin, pyrilamine, nordihydroguaiacetic acid, acivicin, trimethobenzoic acid and capsaicin. Constrictor effects of the dust extracts were inhibited by a wide variety of these agents. Atropine consistently and strikingly reduced the contractile effects of these extracts. This observation may suggest an interaction of the extracts with parasympathetic nerves or more directly with muscarinic receptors. Inhibition of contraction by blocking other mediators was less effective and varied with the dust extract. Pretreatment with capsaicin did not change the constrictor effects of latex 1 but enhanced the effects of latex 2. Depletion of neuropeptides, however, did not reduce the constrictor effect. We suggest that latex extracts cause dose-related airway smooth muscle constriction by nonimmunological mechanisms involving a variety of airway mediators and possibly cholinergic receptors. This effect is not dependent on the presensitization of guinea pigs.

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“…The data illustrated by these studies are similar to those of our previous studies with different organic dust extracts such as coffee, soy, spices, animal food, poultry and swine confinement building dust, brewery dust (hops, barley, yeast), as well as textile dust (cotton, hemp and wool) [23][24][25][35][36][37][38][39][40][41][42]. The pharamcologic studies of paper dust extract on isoalted guinea pig tracheal smooth muscle suggest a complex effect of this airway irritant.…”

Section: Discussionsupporting

confidence: 73%

Effects of Recycled Paper Dust Extracts on Isolated Guinea Pig Trachea

Schachter

Zuśkin

Rienzi

et al. 1998

Lung

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The effect of paper dust collected at two different locations in a paper recycling plant (PD1 and PD2) on isolated nonsensitized guinea pig tracheal smooth muscle was studied in vitro. Dust extracts were prepared as a 1:10 w/v aqueous solution. Dose-related contractions of guinea pig tracheal rings were elicited with both PD1 and PD2. Pharmacologic studies were performed with atropine (10(-6) M), indometacin (10(-6) M), pyrilamine (10(-6) M), LY171883 (10(-5) M), nordihydroguaiaretic acid (10(-5) M), and TMB8 (10(-5) M). The possible role of endogenous neuropeptides in this constrictor process was studied by depleting neural mediators with capsaicin (5 x 10(-6) M) before challenge with dust extracts. Constrictor effects were partially inhibited by a wide variety of the mediator blocking agents. The effects of both extracts were almost totally inhibited by the anticholinergic agent atropine, suggesting that a principal pathway mediating this response may involve the parasympathetic nervous system. The intracellular calcium-blocking agent TMB8 also induced a reduction of the contractile responses to PD1 and PD2 consistent with the well established role of intracellular calcium in smooth muscle constriction. Pretreatment with capsaicin significantly increased the contractile activity of paper dust extracts but only at the higher doses of these extracts. This suggests that the effect of paper dust is not initiated by the release of mediators stored in sensory nerves but that the prerelease of these mediators may enhance the constrictor effects of these dusts. We suggest that paper dust extracts cause dose-related airway smooth muscle constriction possibly associated with the release of cholinergic as well as other mediators. The constrictor effect does not require tissue presensitization or the release of neuropeptides from sensory nerves.

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Pharmacological characterisation of extracts of coffee dusts.

Cited by 11 publications

References 16 publications

Immunological and respiratory changes in animal food processing workers

Immunological and respiratory changes in animal food processing workers

Pharmacologic Characterization of Latex Extracts by Isolated Guinea Pig Tracheal Tissue

Effects of Recycled Paper Dust Extracts on Isolated Guinea Pig Trachea

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