Pharmacological characteristics of Artemisia vulgaris L. in isolated porcine basilar artery

Nguyen, Ha Thi Thanh; Nguyen, Hang; Islam, Md. Zahorul; Obi, Takeshi; Pothinuch, Pitchaya; Zar, Phyu Phyu Khine; Hou, De‐Xing; Nguyen, Thanh Van; Nguyen, Tuong Manh; Dao, Cuong Van; Shiraishi, Masayuki; Miyamoto, Atsushi

doi:10.1016/j.jep.2016.02.009

Cited by 14 publications

(9 citation statements)

References 28 publications

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“…This mobilization induced by MeHg was monitored by means of Calcium kit II -Fluo 4 (Dojindo) as previously described [ 26 , 27 ]. Cells in a 96-well plate were incubated with 2.5 µ M fluo-4 AM for 1 hr at 37°C.…”

Section: Methodsmentioning

confidence: 99%

The MARCKS protein amount is differently regulated by calpain during toxic effects of methylmercury between SH-SY5Y and EA.hy926 cells

Dao

Shiraishi

Miyamoto

2017

The Journal of Veterinary Medical Science

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Methylmercury (MeHg) is an environmental pollutant that shows severe toxicity to humans and animals. However, the molecular mechanisms mediating MeHg toxicity are not completely understood. We have previously reported that the MARCKS protein is involved in the MeHg toxicity to SH-SY5Y neuroblastoma and EA.hy926 vascular endothelial cell lines. In addition, calpain, a Ca2+-dependent protease, is suggested to be associated with the MeHg toxicity. Because MARCKS is known as a substrate of calpain, we studied the relation between calpain activation and cleavage of MARCKS and its role in MeHg toxicity. In SH-SY5Y cells, MeHg decreased cell viability along with increased calcium mobilization, calpain activation and a decrease in MARCKS amounts. However, pretreatment with calpain inhibitors attenuated the decrease in cell viability and MARCKS amount induced only by 1 µM but not by 3 µM MeHg. In cells with a MARCKS knockdown, calpain inhibitors failed to attenuate the decrease in cell viability caused by MeHg. In EA.hy926 cells, although MeHg caused calcium mobilization and a decrease in MARCKS levels, calpain activation was not observed. These results indicate that the participation of calpain in the regulation of MARCKS amounts is dependent on the cell type and concentration of MeHg. In SH-SY5Y cells, calpain-mediated proteolysis of MARCKS is involved in cytotoxicity induced by a low concentration of MeHg.

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Section: Methodsmentioning

confidence: 99%

The MARCKS protein amount is differently regulated by calpain during toxic effects of methylmercury between SH-SY5Y and EA.hy926 cells

Dao

Shiraishi

Miyamoto

2017

The Journal of Veterinary Medical Science

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“…Serotonin ( 29 ) that controls both inhibitory and excitatory neurotransmission was obtained from A . vulgaris by Nguyen et al (). Serotonin is monoamine neurotransmitter and works as 5‐HT receptors, which play an important physiological function in the central and peripheral nervous systems.…”

Section: The Chemistry and Pharmacology Of Artemisia Alkaloids/alliedmentioning

confidence: 99%

“…References are numbered as follows: 1 (Su et al, ), 2 (He at el. 2012), 3 (Su et al, ), 4 (Fontaine et al, ), 5 (Sham'yanov et al, ), 6 (Wang et al, ), 7 (Ma, Sun, Zhou, Hong, & Pei, ), 8 (Shukla et al, ), 9 (Lu et al, ), 10 (Liu et al, & 2017), 11 (Zou et al, ), 12 (Choi et al, ), 13 (Mamun et al, ), 14 (Giang et al, ), 15 (Bourgou et al, ), 16 (Nguyen et al, ), 17 (Jakupovic et al, ), 18 (Lin et al, 2014), 19 (Tu et al, ), 20 (Nakamura & Hattori, ), 21 (Saadali et al, ), 22 (Yamada et al, ), 23 (Wheeler et al, ), 24 (Rashid et al, ), 25 (Umano et al, ), 26 (Bak et al, ), 27 (Kavvadias et al, ), 28 (Megdiche‐Ksouri et al, ), 29 (Kim et al, ), 30 (Choi et al, ), 31 (Geng et al, ), and 32 (Mizushina et al, ).…”

Section: Introductionmentioning

confidence: 99%

The chemistry and pharmacology of alkaloids and allied nitrogen compounds fromArtemisiaspecies: A review

Rashid

Alamzeb²,

Ali³

et al. 2019

Phytotherapy Research

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Several reviews have been published on Artemisia's derived natural products, but it is the first attempt to review the chemistry and pharmacology of more than 80 alkaloids and allied nitrogen compounds obtained from various Artemisia species (covering the literature up to June 2018). The pharmacological potential and unique skeleton types of certain Artemisia's alkaloids provoke the importance of analyzing Artemisia species for bioactive alkaloids and allied nitrogen compounds. Among the various types of bioactive Artemisia's alkaloids, the main classes were the derivatives of rupestine (pyridine–sesquiterpene), lycoctonine (diterpene), pyrrolizidine, purines, polyamine, peptides, indole, piperidine, pyrrolidine, alkamides, and flavoalkaloids. The rupestine derivatives are Artemisia's characteristic alkaloids, whereas the rest are common alkaloids found in the family Asteraceae and chemotaxonomically links the genus Artemisia with the tribes Anthemideae. The most important biological activities of Artemisia's alkaloids are including hepatoprotective, local anesthetic, β‐galactosidase, and antiparasitic activities; treatment of angina pectoris, opening blocked arteries, as a sleep‐inducing agents and inhibition of HIV viral protease, CYP450, melanin biosynthesis, human carbonic anhydrase, [3H]‐AEA metabolism, kinases, and DNA polymerase β1. Some of the important nitrogen metabolites of Artemisia include pellitorine, zeatin, tryptophan, rupestine, and aconitine analogs, which need to be optimized and commercialized further.

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“…Besides the characteristic sensory properties and bitterness, the main interest in the liqueurs and infusions is related to the beneficial effects of the extracts on health and diverse bioactive properties. The scientific literature reports evidence of Artemisia species bioactive compounds and their antihypertensive [ 12 ] antitumoral [ 13 ], anti-inflammatory [ 14 ], hepatoprotective [ 15 ], hypoglycaemic [ 16 ], hypolipidemic [ 17 ], and antioxidant properties [ 18 , 19 ]. The biological properties ascribed to this species are mainly related to thujones, terpenoid ketones that can be found principally in the small, yellow flower heads but, also, in lower concentrations, leaf apices [ 20 ], and phenolic compounds [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Wild and Micropropagated Artemisia eriantha Infusions: In Vitro Digestion Effects on Phenolic Pattern and Antioxidant Activity

Rocchi,

Pellegrini,

Pittia

et al. 2023

Plants

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This study investigated the in vitro simulated gastrointestinal digestion (GID) effects on wild and micropropagated Apennines Genepì infusions. Wild and micropropagated infusions were compared for their antioxidant activity, phenolic contents, and polyphenolic profiles before and after GID. Before digestion, the wild infusions had higher amounts of phenolic compounds and antioxidant activity than the micropropagated ones. Instead, after digestion, the differences in the total phenolic content (TPC) and antioxidant activity between wild and micropropagated infusions were less pronounced. The changes in the TPC and phenolic profiles revealed the presence of several chemical transformations and rearrangements that resulted in compounds with different reactivity and antioxidant potential. Without enzyme actions, the wild infusion digest undergoes higher modifications than those obtained from the micropropagated ones. The current study offers the first concrete proof of the impact of GID on the polyphenolic chemicals present in infusions of wild and micropropagated Apennines Genepì and their antioxidant properties. Our findings are essential for future in-depth analyses of Apennine Genepì infusions and their potential impacts on human health.

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Pharmacological characteristics of Artemisia vulgaris L. in isolated porcine basilar artery

Cited by 14 publications

References 28 publications

The MARCKS protein amount is differently regulated by calpain during toxic effects of methylmercury between SH-SY5Y and EA.hy926 cells

The MARCKS protein amount is differently regulated by calpain during toxic effects of methylmercury between SH-SY5Y and EA.hy926 cells

The chemistry and pharmacology of alkaloids and allied nitrogen compounds fromArtemisiaspecies: A review

Wild and Micropropagated Artemisia eriantha Infusions: In Vitro Digestion Effects on Phenolic Pattern and Antioxidant Activity

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