Pharmacological characterization and regional distribution of α-noradrenergic binding sites of rat spinal cord

Giron, Louis T.; McCann, Shirley A.; Crist-Orlando, Shirley G.

doi:10.1016/0014-2999(85)90701-0

Cited by 23 publications

(8 citation statements)

References 13 publications

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“…Using horseradish peroxidase, the retrograde transport method has clearly demonstrated descending NA fibers originating in the LC and terminating in the sacral intermediolat-era1 region of rats and monkeys (Loewy et al, 1979a;Westlund and Coulter, 1980;Westlund et al, 1982). Alpha,-adrenergic receptors portrayed in the sacral cord correspond to the innervation density of the NA pathway (Gion et al, 1985). As such, these morphological results further support the concept that (major)…”

Section: Noradrenergic Pathway To the Sacral Parasympathetic Centersupporting

confidence: 67%

Locus coeruleus noradrenergic neurons as a micturition center

Sasa¹,

Yoshimura²

1994

Microscopy Res & Technique

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The micturition reflex arch is composed of an afferent pathway from the urinary bladder to the pontine micturition center via the pelvic nerve and spinal cord. The efferent pathway projects from the center to the bladder through the sacral parasympathetic center of intermediolateral column cells. The pontine micturition center is thought to be noradrenergic (NA) neurons in the locus coeruleus (LC) due to the following observations: (1) LC stimulation induces bladder contraction in cats, and this response is blocked by intrathecal application of alpha 1-adrenergic antagonist (prazosin), but not the alpha 2- nor beta-adrenergic antagonist. Although this contraction is not observed after NA depletion with reserpine, with subsequent i.v. injection of L-dopa a NA precursor induces recurrence of the response. (2) The micturition reflex induced by bladder distention is similarly reversed with alpha 1-adrenergic antagonist and chemical destruction of NA cells by injecting 6-hydroxydopamine in the LC. However, subsequently applied alpha 1-adrenergic agonist induces the contraction due to bladder distention. (3) LC stimulation elicits spike generation of sacral intermediolateral cells. Microiontophoretically applied alpha 1-adrenergic antagonist inhibits the LC stimulation-induced spikes of the neuron, which is not antidromically activated by pelvic nerve stimulation. However, spikes in neurons activated antidromically were not affected by the drug. This indicates that the former and latter neurons are interneuron and parasympathetic projecting neurons, respectively. The existence of NA terminals from the LC and alpha 1-adrenergic receptors in the intermediolateral column cells supports the concept that NA cells in the LC are units constituting the micturition center.

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Section: Noradrenergic Pathway To the Sacral Parasympathetic Centersupporting

confidence: 67%

Locus coeruleus noradrenergic neurons as a micturition center

Sasa¹,

Yoshimura²

1994

Microscopy Res & Technique

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“…Physiological, anatomical, and pharmacological studies (Giron et al, 1985;Sullivan et al, 1987;Simmons and Jones, 1988;Yoshimura et al, 1990;Marks et al, 1990) have shown that both a1 and a2 binding sites are present in the rat spinal cord; but to our knowledge no detailed data are yet available concerning their topographic localization according to the subregions of the spinal cord gray matter and to the rostrocaudal level.…”

Section: Introductionmentioning

confidence: 95%

“…Physiological effects of NA systems are classically mediated by adrenoceptors which have been classified (Starke and Langer, 1979), independently of their location or function, into alpha l (al) and alpha 2 (a2) subtypes, according to their relative affinities for agonists and antagonists. Physiological, anatomical, and pharmacological studies (Giron et al, 1985;Sullivan et al, 1987;Simmons and Jones, 1988;Yoshimura et al, 1990;Marks et al, 1990) have shown that both a1 and a2 binding sites are present in the rat spinal cord; but to our knowledge no detailed data are yet available concerning their topographic localization according to the subregions of the spinal cord gray matter and to the rostrocaudal level.…”

Section: Introductionmentioning

confidence: 97%

Normal distribution of alpha‐1‐adrenoceptors in the rat spinal cord and its modification after noradrenergic denervation: A quantitative autoradiographic study

Roudet

Savasta

Feuerstein

1993

J of Neuroscience Research

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The distribution of alpha 1 (alpha 1)-adrenoceptors along the different segments of the spinal cord (cervical, thoracic, lumbar, and sacral) of normal rats has been studied by quantitative autoradiography using the specific alpha 1-antagonist [3H]Prazosin as a ligand. In addition, the influence of noradrenergic (NA) denervation [obtained either by complete transection of the spinal cord at vertebrae level T8-T9 or by selective lesion of NA spinal cord system carried out by intracisternal injection of 6-hydroxydopamine (6-OHDA)] on eventual variations of alpha 1-adrenoceptor density at spinal cord target cells was studied in parallel. In control rats, the quantitative analysis of alpha 1-adrenoceptor densities revealed a widespread distribution of these receptors along all segments of the spinal cord with a similar pattern in the various subregions of gray matter studied. This distribution of alpha 1-adrenoceptors was quite well correlated with the distribution of NA terminals, when referring to previous descriptions by immunohistochemistry. After 6-OHDA lesion, as well as caudally to the transection, a significant increase of alpha 1-adrenoceptor densities was observed in all spinal subregions thus evidencing supersensitivity. These results suggest that NA may act in the spinal cord, at least partly, via alpha 1-adrenoceptors and that the expression of these receptors could be influenced by NA dysfunction, as demonstrated here through the effects observed in lesioned animals.

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“…At the other end of the spectrum are areas of no or very little labeling: granule cells of the hippocampal dentate gyrus, granular layer of the cerebellar cortex, and most white matter (e.g., corpus callosum, pyramidal tract). alpha-1 Adrenergic receptors are also present in the spinal cord, as demonstrated by membrane binding (Jones et al, 1982;Jones and Ademe, 1985;Giron et al, 1985); the finer anatomical localization of these receptors, however, is not known. Both membrane binding (Ebersolt et al, 1981) and autoradiography have revealed the presence of alpha-1 adrenergic receptors on glial cells.…”

Section: Anatomical Distributionmentioning

confidence: 99%

The alpha-1 Adrenergic Receptors

Ruffolo¹

1987

The Receptors

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All rights reservedNo part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise without written permission from the Publisher.Preface vii ology will allow alpha-l adrenergic receptors to be isolated and purified and their amino acid sequence to be identified. This will ultimately allow for the systematic subclassification of alpha-l adrenergic receptors and for their differential regulation to be studied in greater detail. These new advances are likely to take place within the next five years, and it is our intent for this review of alpha-l adrenergic receptor pharmacology to provide an accurate summary of the current state of knowledge relating to alpha-l adrenergic receptors, which we hope will serve as a foundation upon which to build a broader base of knowledge in the future.

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Pharmacological characterization and regional distribution of α-noradrenergic binding sites of rat spinal cord

Cited by 23 publications

References 13 publications

Locus coeruleus noradrenergic neurons as a micturition center

Locus coeruleus noradrenergic neurons as a micturition center

Normal distribution of alpha‐1‐adrenoceptors in the rat spinal cord and its modification after noradrenergic denervation: A quantitative autoradiographic study

The alpha-1 Adrenergic Receptors

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