Pharmacological characterization of the ATP-dependent low Km guanosine 3′,5′-cyclic monophosphate (cGMP) transporter in human erythrocytes

Sundkvist, Elisabeth; Jaeger, Ragnhild; Sager, Georg

doi:10.1016/s0006-2952(01)00940-6

Cited by 39 publications

(28 citation statements)

References 24 publications

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“…In Drosophila tubules, both cGMP and cAMP transport were both reduced at high concentrations of methotrexate; this reduction became statistically significant at a concentration of 4·mmol·l -1 . Although the ABCG inhibitor mitoxantrone has not been shown to affect cGMP transport in erythrocytes (Sundkvist et al, 2002), treatment of tubules with mitoxantrone did impact on cGMP transport; at 20·mol·l -1 the ratio of cGMP transport by tubules was reduced to 27% of that of untreated control tubules (Table·1).…”

Section: Abcgmentioning

confidence: 95%

A new role for a classical gene: White transports cyclic GMP

Evans

Day

Cabrero

et al. 2008

Journal of Experimental Biology

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SUMMARYGuanosine 3Ј-5Ј cyclic monophosphate (cGMP) and adenosine 3Ј-5Ј cyclic monophosphate (cAMP) are important regulators of cell and tissue function. However, cGMP and cAMP transport have received relatively limited attention, especially in model organisms where such studies can be conducted in vivo. The Drosophila Malpighian (renal) tubule transports cGMP and cAMP and utilises these as signalling molecules. We show here via substrate competition and drug inhibition studies that cAMP transport -but not cGMP transport -requires the presence of di-or tri-carboxylates; and that transport of both cyclic nucleotides occurs via ATP binding cassette sub-family G2 (ABCG2), but not via ABC sub-family C (ABCC), transporters. In Drosophila, the white (w) gene is known for the classic eye colour mutation. However, gene expression data show that of all adult tissues, w is most highly expressed in Malpighian tubules. Furthermore, as White is a member of the ABCG2 transporter class, it is a potential candidate for a tubule cGMP transporter. Assay of cGMP transport in w -(mutant) tubules shows that w is required for cGMP transport but not cAMP transport. Targeted over-expression of w in w -tubule principal cells significantly increases cGMP transport compared with that in w -controls. Conversely, treatment of wild-type tubules with cGMP increases w mRNA expression levels, implying that cGMP is a physiologically relevant substrate for White. Immunocytochemical localisation reveals that White is expressed in intracellular vesicles in tubule principal cells, suggesting that White participates in vesicular transepithelial transport of cGMP.

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Section: Abcgmentioning

confidence: 95%

A new role for a classical gene: White transports cyclic GMP

Evans

Day

Cabrero

et al. 2008

Journal of Experimental Biology

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“…Studies with human erythrocytes have shown that the organic anionic pump which transports cGMP to extracellular area is dependent on a) ATP, b) its hydrolysis, and on c) the stimulation of ATPase activity by the cGMP itself (Sundkvist et al, 2002). PDE inhibitors block this transport.…”

Section: Vas Deferensmentioning

confidence: 99%

“…Sildenafil inhibiting PDE5 increases cGMP in the vas deferens muscular fibers and additionally blocks the activity of this organic anion pump. The overall result is an increase in the intracellular cGMP by a) prevention of the destruction of cGMP and b) inhibition of cGMP extracellular export (Sundkvist et al, 2002).…”

Section: Vas Deferensmentioning

confidence: 99%

The Role of PDE5 Inhibitors in the Treatment of Testicular Dysfunction

Dimitriadis¹,

Baltogiannis²,

Koukos³

et al. 2012

Male Infertility

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“…To date, most research has focused on the effect of sildenafil on vascular events in penile corpora cavernosa, but there is increased interest in potential efficacy of sildenafil in modulating cyclic nucleotide signaling in other tissues. [4][5][6][7][8][9][10][11] In addition, biochemical characteristics of the interaction of sildenafil with PDE5 and perhaps other proteins are still poorly understood. Investigation of these basic processes is compromised by the fact that purified sildenafil is not commercially available.…”

Section: Introductionmentioning

confidence: 99%