Pharmacological effects of lysozyme on COPD and bronchial asthma with sputum: A randomized, placebo-controlled, small cohort, cross-over study

Ohbayashi, Hiroyuki; Setoguchi, Yasuhiro; Fukuchi, Yoshinosuke; Shibata, Kenichi; Sakata, Yukinori; Arai, Toshihisa

doi:10.1016/j.pupt.2016.03.001

Cited by 15 publications

(14 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, the precise contribution of lysozyme to antibacterial defence in different tissues is still lacking and many aspects concerning its non-catalytic biological role are not completely understood. An interesting development concerns the possibility to attribute to lysozyme additional functions, besides antibacterial defence, such as a potent anti-inflammatory activity [29,35,36]. Lysozyme significantly suppresses the production of the inflammatory cytokines TNF-α, IL-1β and IL-6 (Interleukin 6) [37,38,39], independently from muramidasic activity, because of the peptide motifs found in the N-terminal region of the protein [40].…”

Section: Discussionmentioning

confidence: 99%

“…The wide number of biological roles of lysozyme and the significant effects against bacterial and viral infections, depression of immune response and phlogosis, led to the commercialization and employment of HEWL in a number of human pathologies, in which its use is consolidated and safe, since the 1950 (in addition to the indications for which marketing in various countries is authorized, see also: [29,30,31]). The therapeutic effectiveness of lysozyme to control the growth of susceptible bacteria is based on a generally well-known mechanism of action although some aspects, such as the impact of the peptidoglycan fragments released by the catalytic action, and the muramidase-independent properties, remain elusive [32,33].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lysozyme-Induced Transcriptional Regulation of TNF-α Pathway Genes in Cells of the Monocyte Lineage

Bergamo¹,

Gerdol

Pallavicini

et al. 2019

IJMS

View full text Add to dashboard Cite

Lysozyme is one of the most important anti-bacterial effectors in the innate immune system of animals. Besides its direct antibacterial enzymatic activity, lysozyme displays other biological properties, pointing toward a significant anti-inflammatory effect, many aspects of which are still elusive. Here we investigate the perturbation of gene expression profiles induced by lysozyme in a monocyte cell line in vitro considering a perspective as broad as the whole transcriptome profiling. The results of the RNA-seq experiment show that lysozyme induces transcriptional modulation of the TNF-α/IL-1β pathway genes in U937 monocytes. The analysis of transcriptomic profiles with IPA® identified a simple but robust molecular network of genes, in which the regulation trends are fully consistent with the anti-inflammatory activity of lysozyme. This study provides the first evidence in support of the anti-inflammatory action of lysozyme on the basis of transcriptomic regulation data resulting from the broad perspective of a whole-transcriptome profiling. Such important effects can be achieved with the supplementation of relatively low concentrations of lysozyme, for a short time of exposure. These new insights allow the potential of lysozyme in pharmacological applications to be better exploited.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lysozyme-Induced Transcriptional Regulation of TNF-α Pathway Genes in Cells of the Monocyte Lineage

Bergamo¹,

Gerdol

Pallavicini

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…Thus, it can be considered that lysozyme that has avoided being degraded by proteases in digestive tract could be absorbed as an intact form and shows anti-inflammatory effect in vivo. In fact, it has been reported that oral intake of lysozyme is capable of exerting its pharmacological activity (Ohbayashi et al 2016), suggesting that part of orally administered lysozyme reaches the intestine as an intact form and exerts its anti-inflammatory activity.…”

Section: Effect Of Lysozyme On the Signaling Pathways In Macrophagesmentioning

confidence: 99%

Anti-inflammatory effect of lysozyme from hen egg white on mouse peritoneal macrophages

et al. 2018

View full text Add to dashboard Cite

Lysozyme from hen egg has been reported to possess an anti-inflammatory effect. However, little is known about its detailed mechanism. The mechanism of anti-inflammatory effect of lysozyme was examined in this study. When mouse macrophage-like cell line RAW264.7 cells and mouse peritoneal macrophages were activated with lipopolysaccharide (LPS) and then treated with lysozyme, the production of tumor necrosis factor-α and interleukin-6 was significantly suppressed. The effect was induced by suppressing the gene expression levels of both cytokines. Phagocytosis activity of peritoneal macrophages was not altered by the treatment with lysozyme, suggesting that lysozyme shows the anti-inflammatory effect without inhibiting the phagocytotic response of macrophages. In addition, lysozyme inhibited phosphorylation of c-jun N-terminal kinase (JNK) and was taken up by macrophages within 1 h after treatment of the cells with lysozyme. Overall results suggest that lysozyme is taken up intracellularly and suppresses LPS-induced inflammatory responses by inhibiting JNK phosphorylation.

show abstract

“…8 Furthermore, a recent double-blind, placebo-controlled, crossover study on patients with COPD demonstrated significantly greater improvements in forced expiratory volume in 1 second (FEV 1 ), impulse-oscillation-system-assessed peripheral pulmonary function, and COPD assessment test (CAT) scores in those receiving a combination of lysozyme with regular treatment than in those receiving regular treatment alone. 9 …”

Section: Introductionmentioning

confidence: 99%

Prevention of COPD exacerbation by lysozyme: a double-blind, randomized, placebo-controlled study

Fukuchi¹,

Inoue²,

Sakata³

et al. 2016

COPD

Self Cite

View full text Add to dashboard Cite

Background/aimLysozyme (mucopeptide N-acetyl-muramyl hydrolase) is widely used as a mucolytic and anti-inflammatory agent in Japan. We evaluated the effects of long-term lysozyme administration on COPD exacerbation.MethodsIn a 1-year, randomized, double-blind, placebo-controlled, parallel trial, patients with moderate-to-severe COPD and one or more episodes of COPD exacerbation in the previous year before enrollment were selected. Lysozyme (270 mg) or placebo was administered orally for 52 weeks as an add-on to the standard therapies such as bronchodilators. COPD exacerbation, pulmonary function, and COPD assessment test scores were analyzed. An exacerbation was defined as worsening of more than one symptom of COPD (cough, sputum volume, purulent sputum, or breathlessness) leading to a change in medication. The primary endpoint was exacerbation rate.ResultsA total of 408 patients were randomly assigned to the lysozyme and placebo groups. The baseline characteristics were similar between the two groups. The exacerbation rate was not significantly different between the two groups (1.4 vs 1.2; P=0.292, Poisson regression). However, a subgroup analysis showed that lysozyme might reduce exacerbation rate in patients with airway-dominant phenotype (1.2 vs 1.6). Moreover, the median time to first exacerbation was longer in patients with airway-dominant phenotype in the lysozyme group than that in the placebo group. The levels of improvement in forced expiratory volume in 1 second and COPD assessment test scores were not statistically different between the groups, but were always greater in the lysozyme group than in the placebo group over the 52 weeks of the study.ConclusionThe effects of using lysozyme as an add-on to standard COPD therapy were not significantly different compared with placebo and were insufficient to prevent COPD exacerbation.

show abstract

Pharmacological effects of lysozyme on COPD and bronchial asthma with sputum: A randomized, placebo-controlled, small cohort, cross-over study

Cited by 15 publications

References 19 publications

Lysozyme-Induced Transcriptional Regulation of TNF-α Pathway Genes in Cells of the Monocyte Lineage

Lysozyme-Induced Transcriptional Regulation of TNF-α Pathway Genes in Cells of the Monocyte Lineage

Anti-inflammatory effect of lysozyme from hen egg white on mouse peritoneal macrophages

Prevention of COPD exacerbation by lysozyme: a double-blind, randomized, placebo-controlled study

Contact Info

Product

Resources

About