Pharmacological Effects of the Seed Extract of Trigonella foenum-graecum L. on Cardiovascular and Anxiety Diseases

Abstract

The main objective of this study was to determine the antioxidant activity of Trigonella foenum-grecum L. seeds. The significance of the bioactive compounds found in these seeds was evaluated by GC-MS. The 2,2-diphenyl-1-picrylhydrazyl (DPPH), Superoxide dismutase (SOD), and Catalase (CAT) tests are common ways to assess antioxidant activity. The concentration of the sample required to scavenge 50% of free radicals was calculated using a graph of per cent inhibition versus concentration. The IC50 values for 2,2-diphenyl 1-picryl-hydrazyl (DPPH) radical activity in aqueous and methanolic extracts were found to be 2.8741 and 3.8372, respectively. The figure is nearly identical to the control value for ascorbic acid, 2.5391. Catalase (CAT) specific activity and per cent inhibition for aqueous extract were found to be 34.227 moles of hydrogen peroxide H2O2/min/ml and 77.38 per cent, respectively. Catalase (CAT) specific activity and per cent inhibition for methanolic extract were evaluated to be 26.0153 moles of H2O2/min/ml and 82.42 per cent, respectively. The percentage of inhibition and the specific activity of SOD in aqueous extract were found to be 53.21% and 1.5843 units/mg, respectively. Superoxidedismutase (SOD) specific activity and per cent inhibition for methanolic extract were 1.3848 units/mg and 61.24 per cent, respectively. This showed that the antioxidant activity of the Trigonella foenum-grecum L. seed methanolic extract was higher than that of the aqueous extract. The results of this in vitro study showed that this species of Trigonella foenum-grecum L. is the greatest remedy for cardiovascular illness and suitable for use as a raw pharmaceutical (CVD). Further research regarding the antioxidant capacity of the seeds from this species in a living system may be performed in vivo.