Pharmacological evaluation of ameliorative effect of aqueous extract of Cucumis sativus L. fruit formulation on wound healing in Wistar rats

Abstract

Aim:The aim of present investigation was to formulate and evaluate the ameliorative effect of aqueous extract of Cucumis sativus L. fruit cream formulation on experimentally induced wounds in rats. Materials and Methods: The cream was formulated using soft white paraffin base containing 2.5%, 5%, and 10% w/w of aqueous extract of of Cucumis sativus L. fruit. Excision wounds of size 300 mm 2 and 2 mm depth were used for the study of rate of contraction of wound and epithelization. All the three formulations were evaluated for various pharmaceutical parameters such as pH, viscosity, spreadability, and acute skin irritation study. Epithelialization period, wound contraction, scar width, and histopathological evaluation parameters were used for pharmacological evaluation of wound healing activity of the formulation. Statistical Analysis: All the results were expressed as mean±SEM. Data analysis was performed using GraphPad Prism 5.0 software (GraphPad, San Diego, CA, USA). Statistical comparisons were made between drug-treated groups and disease control animals. Data of disease activity index were analyzed using one-way analysis of variance; Dunnett's multiple range test was applied for post hoc analysis, whereas data of wound area and percent wound contraction were analyzed using two-way repeated analysis of variance, Bonferroni's multiple range test was applied for post hoc analysis. A value of P<0.05 was considered to be statistically significant. Results: Cream formulation of AECS when applied topically did not show any sign and symptoms of skin irritation. The treatment with aqueous extract of C. sativus fruit cream formulation (2.5%, 5%, and 10% w/w) resulted in significance decrease (P<0.05, P<0.001, and P<0.001, respectively) in wound area, epithelization period, and scar width, whereas rate of wound contraction significance increased (P<0.001 respectively) when compared with control group animals. Conclusion: The present investigation demonstrates that by virtue of its antioxidant property and presence of the flavanoids content in C. sativus may responsible for wound contraction and elevated rate of epithelization in wound healing in laboratory animals.

Wound healing potential of naringin ointment formulation via regulating the expression of inflammatory, apoptotic and growth mediators in experimental rats

“…Naringin ointment (1, 2, and 4%) for topical application was prepared as described previously (Patil et al, 2011(Patil et al, , 2012d.…”

“…Preliminary evaluation of naringin ointment formulations including pH, viscosity, spreadability, acute skin irritation study, and extrudability at different concentrations was carried out as described by Patil et al (2011Patil et al ( , 2012d). …”

“…It was shown to possess anti-apoptotic potential via modulating the Bax-Bcl-2 pathway thus decreased DNA damage and neuronal apoptosis (Gopinath et al, 2011). It has been documented that free radicals are dynamically involved in the delayed healing process (Patil et al, 2011(Patil et al, , 2012d. The action of naringin in wound healing may be due to antioxidant and radical trapping and scavenging.…”

Hesperidin enhances angiogenesis via modulating expression of growth and inflammatory factor in diabetic foot ulcer in rats

“…8 The process of wound healing is a finely coordinated and controlled process, regulated by numerous factors and is inclusive of-hemostasis, inflammation, granulation, and repair. 9,10 Supporting players in the process of wound healing include blood cellular components, keratinocytes, various growth and proinflammatory mediators, and endothelial cells. The background mechanism of the wound-healing process has been vaguely clarified, but overall anyone of the above factors may modulate or delay the process of wound healing in diabetes.…”

Quantitative Estimation of Cucurbitacin E in Various Extracts of Cucumis Sativus L. By Spectrophotometric Method

“…Cytotoxic, antifungal and antibacterial activity activities have been reported from leaves and stems extracts. [8][9][10][11][12][13][14][15] Colorimetric reactions are widely used in the UV/Vis spectrophotometric method, which is easy to perform, rapid and applicable in routine laboratory use and low cost. Phosphomolybdic acid is widely employed as a reagent in quantitative analysis of several drugs.…”