Pharmacological intervention in acute myocardial ischemia and reperfusion

Vusse, Ger J.; Reneman, Robert S.

doi:10.1016/0165-6147(85)90032-x

Cited by 23 publications

(9 citation statements)

References 13 publications

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“…An excessive intracellular Ca2+ accumulation is considered to be associated with dysfunction and disruption of the ischaemic cardiac cells, leading to leakage of intracellular enzyme from the hearts (Araki & Lefer 1980;Van der Vusse & Reneman 1984;Glover & Sleph 1989). In the present study, reperfusion following ischaemia resulted in a marked release of CPK, and SD-32 I 1 significantly reduced this abnormal reaction.…”

Section: Discussionmentioning

confidence: 99%

ANTI‐ISCHAEMIC AND VASOSPASMOLYTIC EFFECTS OF A NOVEL Ca2+ CHANNEL BLOCKER, SD‐3211, IN VITRO

Miyawaki

Furuta

Shigei

et al. 1991

Clin Exp Pharma Physio

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1. The present study was undertaken to determine the vasospasmolytic activity of a novel non-dihydropyridine type of Ca2+ channel blocker, SD-3211, in isolated canine coronary arteries and its ability to reduce myocardial ischaemic damage in isolated perfused rabbit hearts. 2. The vasospasmolytic effect of SD-3211 was investigated using 3,4-diaminopyridine-induced rhythmic contraction, in comparison with its enantiomer (SD-3212), nicardipine and diltiazem. SD-3211 was shown to reduce the peak tension and increase the contraction frequency. The order of potency for the relaxation of the peak tension was as follows: nicardipine greater than SD-3211 greater than diltiazem greater than SD-3212 and being compatible with that for the relaxant effects of these compounds on KCl-induced contraction in the same specimen. 3. Furthermore, the effect of SD-3211 on myocardial damage due to global ischaemia for 60 min followed by 60 min of reperfusion was examined. SD-3211 at a concentration of 2 X 10(-8) mol/L was given for 40 min before and again for 60 min after the ischaemia. SD-3211 attenuated the increase in leakage of creatine phosphokinase from the hearts and the decrease in pH of perfusate during reperfusion, while concomitantly providing a significant improvement in the post-ischaemic recovery of developed tension. 4. These results suggest that SD-3211 has properties to reduce coronary vasospasm and to provide protection against ischaemic damage, both of which may have beneficial actions in the treatment of ischaemic heart disease.

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Section: Discussionmentioning

confidence: 99%

ANTI‐ISCHAEMIC AND VASOSPASMOLYTIC EFFECTS OF A NOVEL Ca2+ CHANNEL BLOCKER, SD‐3211, IN VITRO

Miyawaki

Furuta

Shigei

et al. 1991

Clin Exp Pharma Physio

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“…The following increase in free cytoplasmic Na + stimulates inversion of ionic flows related with electrogenic Na+/Ca -~*-antiport. Rise of diastolic [Ca2+]oyt leads to the development of arrhythrnias [7]. L-1 inhibits [Na § L rise induced by hypoxia [3].…”

Section: Resultsmentioning

confidence: 99%

Antiarrhythmic properties of a new Suphan-based combined preparation and their possible mechanism of realisation

et al. 1998

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Considerable potentiation of antiarrhythmic and antifibrilation effects of two cardiotonic agents, Suphan and the Na-channel blocker L1, was found in the model experiments in rive with early occlusion and reperfusion arrhythmias. Suphan in combination with L1 inhibited the rise of intraceUular Ca 2+ (by about 70%) in isolated cardiomyocytes incubated under hypoxie conditions. Key words: hypoxia; sodium channel blockers; antiarrhythmic drugs; Ca-exchange; cardiomyocytesCombined action of antiarrhythmic agents in the treatment of cardiac antiarrhythmJa is not adequately investigated. When applied together, some antiarrhythmics, for example, tertiary derivative of Ajmaline (N-propylajmaline-bromide) and local anesthetic Trimecainum in combination with Ethmosine and Ethacisine (all named as Methacisine) potentiate therapeutic effects of each other [1,5]. Combined usage of antiarrhythmic agents with different mechanisms of action allows one to reduce substantially cardiotoxicity and the probability of adverse effects.In this respect, suphan and L-1 (both preparations were synthesised in Russia) seem to be of particular interest. Suphan, di-potassium salt of Nsuccinyl-dl-tryptophan, is a nonglycoside cardiotonic with antianginal and antihypoxia activities. Its antiarrhythmic effect is due predominantly to its action on Ca 2+ exchange in cardiomyocytes [2]. L-l, dimethyl-aminoethyl ether of para-butyl-aminobenzol acid, is a Na-channel blocker by mechanism of action, i.e., it belongs to IB class of antiarrhythmic drugs [8]. MATERIALS AND METHODSAntiarrhythmic activity of the agents was assessed in model experiments with early occlusion and reperfusion arrhythmias on anesthetized eats in rive in complete accordance with the methodical recommendations for experimental (pharmacological) investigation of preparations proposed for clinical trial as agents for cardiac arrhythmia treatment or prevention.The procedures of isolating eardiomyocytes from the left ventricle, then loading with Fura 2-AM, and fluorescence recording were described previously [4]. Intracellular Ca 2 § content was calculated from the following formula:

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“…With blood supply being blocked for the ischaemic testis, the contribution to the ROS pool as such coming from leucocytes may not be necessarily high. Toxic ROS not only leads to cell death (Van der Vusse & Reneman, 1985; Weins & Lucchesi, 1990) but also activates apoptosis (Johnson et al. , 1996).…”

Section: Discussionmentioning

confidence: 99%

Simultaneous increase in germ cell apoptosis and oxidative stress under acute unilateral testicular ischaemia in rats

2003

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Ischaemia induced germ cell apoptosis in rat testis was studied in detail to find out (i) spermatogenic stage or seminiferous epithelium region specific involvement of germ cells in apoptosis, (ii) preferential specificity of a particular germ cell type to become apoptotic and (iii) the ratio of live and dead testicular cells isolated in vitro after various period of ischaemic induction. Cell apoptosis, as observed in histological sections increased from 1 to 24 h of ischaemia. Apoptosis was not restricted to any specific germ cell type but was observed simultaneously in all the cell types in the initial hours (1-6 h) of ischaemia. No spermatogenic stage specific preference in apoptotic induction was also observed. However, as the duration of ischaemia progressed, the cell types observed to be most affected in number and morphology were the spermatids followed by spermatocytes. Centrally located tubules of testis were affected first than those located in the periphery. Overexpression of Bax staining was limited to few germ cell nuclei only. More than 95% of the germ cells in the control testis that earlier showed trypan blue dye exclusion were found stained after 12 h of ischaemia. Starting from early hours (1 h), lipid peroxidation rose proportionally with the duration of ischaemia while superoxide dismutase (SOD) and catalase activities were found decreased. Significant (p < 0.05) increase in the activities of glutathion-s-transferase and levels of hydrogen peroxide were observed after 6 h of ischaemia. These findings indicate that the physiological processes of oxidative stress have a direct linkage to the extent of germ cell apoptosis in the seminiferous epithelium.

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Pharmacological intervention in acute myocardial ischemia and reperfusion

Cited by 23 publications

References 13 publications

ANTI‐ISCHAEMIC AND VASOSPASMOLYTIC EFFECTS OF A NOVEL Ca2+ CHANNEL BLOCKER, SD‐3211, IN VITRO

ANTI‐ISCHAEMIC AND VASOSPASMOLYTIC EFFECTS OF A NOVEL Ca2+ CHANNEL BLOCKER, SD‐3211, IN VITRO

Antiarrhythmic properties of a new Suphan-based combined preparation and their possible mechanism of realisation

Simultaneous increase in germ cell apoptosis and oxidative stress under acute unilateral testicular ischaemia in rats

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