Pharmacological investigation of the nociceptive response and edema induced by venom of the scorpion Tityus serrulatus

Nascimento, Elias B.; Costa, Karina A.; Bertollo, Caryne Margotto; Oliveira, Antônio Carlos Pinheiro de; Rocha, Leonardo Cristian; Souza, Adriano Lopes de; Glória, Maria Beatriz Abreu; Moraes-Santos, Tasso; Coelho, Márcio M.

doi:10.1016/j.toxicon.2004.12.020

Cited by 41 publications

(35 citation statements)

References 39 publications

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“…4) and can be attributed to the presence of 5-HT. These values also correspond to the values of 5-HT reported in Leirus quinquestriatus, Lychas laevifrons (Pock) and Tityus serrulatus, respectively [6][7][8].…”

Section: Discussionsupporting

confidence: 83%

“…Till date, venoms from only three species of scorpions as mentioned above contain 5-HT [6][7][8]. The present study provides the evidence for existence of 5-HT in the venom of the Indian red scorpion.…”

Section: Discussionmentioning

confidence: 52%

“…Some of the above manifestations can be produced by 5-hydroxytryptamine [5]. 5-Hydroxytryptamine (5-HT) has been shown to be present in the venom of Leirus quinquestriatus [6], Lychas laevifrons, Pock [7] and Tityus serrulatus [8] but not in the venom of Indian red scorpion. Therefore, the present study was undertaken to identify and isolate 5-HT in crude MBT venom.…”

Section: Introductionmentioning

confidence: 99%

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Isolation of 5-Hydroxytryptamine from the Venom of the Indian Red Scorpion (Mesobuthus tamulus)

Tiwari¹,

Mandal²,

Kanoo³

et al. 2008

TOTNJ

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The present study was undertaken to isolate 5-hydroxytryptamine (5-HT) from crude Indian red scorpion (Mesobuthus tamulus; MBT) venom as it has not been reported yet. Therefore, with the help of bioassay (using gastric fundus contractions) and fluorimetric assay methods, the 5-HT content of crude MBT venom (CV) was determined. The concentration of 5-HT in CV by either of the methods was different (117 times). Further, a 5-HT antagonist methysergide partially blocked the CV-induced gastric fundus contractions. Hence, for isolation and determination of 5-HT in crude MBT venom, CV was subjected to Sephadex gel (G 75-125) filtration chromatography. The eluates were screened for optical density (OD) at 280 nm, lethality and gastric fundus contractions. Two peaks of enhanced gastric fundus contractions were observed between 26-70 ml and 90 -110 ml of eluate volumes. As the first peak (between 26-70 ml) eluates exhibited high OD and lethality, further analysis was not undertaken. The second peak (between 90-110 ml) eluates exhibited very low OD and were non-lethal. On bioassay, the 5-HT content of pooled eluates of second peak was 0.0398 ± 0.008 g/ml and by fluorimetric assay it was 0.0396 ± 0.007 g/ml. The 5-HT concentration of crude MBT venom per unit mass was 0.0175 ± 0.0035 g/mg by bioassay and 0.0174 ± 0.0031 g/mg by fluorimetric assay. Further, the gastric fundus contractions elicited by second peak eluates were blocked by methysergide (1.0 M). Thus, the second peak eluates of crude MBT venom contain 5-HT and its concentration was about 0.0174 g/mg.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 52%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation of 5-Hydroxytryptamine from the Venom of the Indian Red Scorpion (Mesobuthus tamulus)

Tiwari¹,

Mandal²,

Kanoo³

et al. 2008

TOTNJ

View full text Add to dashboard Cite

show abstract

“…Whereas the observation that the hind paw edema was significantly attenuated only in its later stages of development may indicate that this is the phase in which COX products are acting, it may also simply reflect the kinetics of ketoprofen absorption and the time required for COX inhibition rather than being an indicator of a lack of involvement of COX products in the early phase of the edema. The ability of COX inhibitors to attenuate arthropod venominduced edema has also been reported for experimental envenoming with P. nigriventer (Zanchet et al, 2004) and T. serrulatus (Nascimento Jr. et al, 2005) venoms.…”

Section: Discussionmentioning

confidence: 73%

Pharmacological Characterization of the Edema Caused by Vitalius dubius (Theraphosidae, Mygalomorphae) Spider Venom in Rats

Silva

Linardi

Antunes

et al. 2015

Journal of Pharmacology and Experimental Therapeutics

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Bites by tarantulas (Theraphosidae, Mygalomorphae) in humans can result in mild clinical manifestations such as local pain, erythema, and edema. Vitalius dubius is a medium-sized, nonaggressive theraphosid found in southeastern Brazil. In this work, we investigated the mediators involved in the plasma extravasation caused by V. dubius venom in rats. The venom caused dose-dependent (0.1-100 mg/site) edema in rat dorsal skin. This edema was significantly inhibited by butyl]benzamide) (SR48968, a neurokinin NK 2 receptor antagonist) had no effect on the venom-induced increase in vascular permeability. In rat hind paws, the venom-induced edema was attenuated by ketoprofen (a nonselective COX inhibitor) administered 15 minutes postvenom. Preincubation of venom with commercial antiarachnid antivenom attenuated the venom-induced edema. These results suggest that the enhanced vascular permeability evoked by V. dubius venom involves serotonin, COX products, neurokinin NK 1 receptors, and nitric oxide formation. The attenuation of hind paw edema by ketoprofen suggests that COX inhibitors could be useful in treating the local inflammatory response to bites by these spiders.

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“…Prostaglandina E 2 (PGE 2 ) é o principal PERALES et al, 1992) e pela miotoxina I do veneno de B. asper (GUTIÉRREZ et al, 1986b). Entretanto, outros venenos como o da lagarta Lonomia obliqua (BASTOS et al, 2004), do escorpião Tityus serrulatus (NASCIMENTO Jr. et al, 2005), Bothrops asper em ratos (CHACUR et al, 2001), e Bothrops lanceolatus em ratos ) não contam com a participação desta via na formação do edema.…”

Section: Os Resultados Mostraram Que O Veneno Deunclassified

Alterações locais induzidas pela secreção tóxicas de Philodryas patagoniensis (Girard, 1857) (Serpentes: Colubridae)

Lopes¹

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Pharmacological investigation of the nociceptive response and edema induced by venom of the scorpion Tityus serrulatus

Cited by 41 publications

References 39 publications

Isolation of 5-Hydroxytryptamine from the Venom of the Indian Red Scorpion (Mesobuthus tamulus)

Isolation of 5-Hydroxytryptamine from the Venom of the Indian Red Scorpion (Mesobuthus tamulus)

Pharmacological Characterization of the Edema Caused by Vitalius dubius (Theraphosidae, Mygalomorphae) Spider Venom in Rats

Alterações locais induzidas pela secreção tóxicas de Philodryas patagoniensis (Girard, 1857) (Serpentes: Colubridae)

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