Pharmacological mechanism underlying the antinociceptive activity of vanillic acid

Yrbas, María de los Angeles; Morucci, Florencia; Alonso, Rosario; Gorzalczany, Susana

doi:10.1016/j.pbb.2015.02.016

Cited by 25 publications

(10 citation statements)

References 32 publications

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“…In the phytochemical group tests of leaf extract, some major phytochemicals namely; alkaloids, steroids, glycosides, tannins, and flavonoids were identified whereas glycosides, flavonoids, alkaloids, tannin, saponins and gum were present in the stem extract. Besides, polyphenolic constituents namely (+)-catechin, ellagic acid and vannilin identified in the HPLC analysis, which showed analgesic activity in previous studies [34,35]. Presence of slight increased amount of polyphenols as well as their synergistic action in stem extract may be responsible for more potent as an analgesic agent than other extracts.…”

Section: Discussionmentioning

confidence: 84%

Bioactivities of Bruguiera gymnorrhiza and profiling of its bioactive polyphenols by HPLC-DAD

et al. 2017

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Background: In folk medicine leaves and stem of Bruguiera gymnorrhiza (L.) are commonly used to treat diarrhea, fever, diabetes, pain and a number of ailments. The present study was carried out to explore antioxidant, analgesic and antidiarrhoeal activities of ethanol extract of leaves and stem of B. gymnorrhiza and also to analyze its major bioactive natural polyphenols by HPLC-DAD. Methods: Total polyphenol content was spectrophotometrically determined using Folin Chiocalteu's reagent while the flavonoids by aluminum chloride colorimetric assay. Antioxidant activity was determined by DPPH free radical scavenging, reducing power, nitric oxide and hydrogen peroxide scavenging assays. Identification and quantification of bioactive polyphenols were done by HPLC-DAD method. Antidiarrhoeal activity of the extracts was evaluated using experimentally castor oil induced diarrhea in mice. Acetic acid induced writhing method was used to evaluate the analgesic activity. Acute oral toxicity and brine shrimp lethality assay were performed to check the cytotoxic potential. Results: Both the leave and stem extracts contain significant amount of phenolic and flvonoid content. Extracts showed DPPH radical scavenging, nitric oxide, hydrogen peroxide scavenging and also concentration dependent reducing power activity. HPLC analysis of both extract indicated the presence of significant amount of vanillic acid along with other phenolic constituents. Both extracts showed significant (P < 0.01) analgesic and antidiarrhoeal activity. Furthermore, extracts showed negligible toxic effect. Conclusion: Along with other phenolic compounds, vanillic acid present in the extract may be responsible for antioxidant, analgesic and antidiarrhoeal activities. Altogether these results rationalize the use of this plant in traditional medicine.

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Section: Discussionmentioning

confidence: 84%

Bioactivities of Bruguiera gymnorrhiza and profiling of its bioactive polyphenols by HPLC-DAD

et al. 2017

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“…Vanillic and ferulic acids are phenolic acids, while p ‐coumaric acid belongs to phenylpropanoic acids and 7‐hydroxycoumarin is one of the polyphenolic components (Li et al., ; Yrbas, Morucci, Alonso, & Gorzalczany, ). It has been reported that in wine, these phytochemicals are involved in the interaction between flavor substances and oral sensation (Esteban‐Fernández, Muñoz‐González, Jiménez‐Girón, Pérez‐Jiménez, & Pozo‐Bayón, ).…”

Section: Resultsmentioning

confidence: 99%

Dynamic effects of fermentation on phytochemical composition and antioxidant properties of wampee (Clausena lansium (Lour.) Skeel) leaves

Chang

Guo

et al. 2018

Food Science & Nutrition

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Variations in the phytochemical composition and antioxidant properties were studied in the wine of wampee leaves obtained at different stages of fermentation process. The highest concentrations of total phenolic and flavonoid contents were attained at Day 12 and Day 21 of fermentation, respectively. In addition, five phytochemical compounds including vanillic acid, p‐coumaric acid, rutin, ferulic acid, and 7‐hydroxycoumarin were identified and quantified by HPLC in fermented wampee products. The strongest antioxidant activity in wine was monitored on Day 12. Furthermore, total antioxidant activity was significantly correlated with vanillic acid, p‐coumaric acid, ferulic acid, and 7‐hydroxycoumarin compared with rutin. The obtained results suggested that 12‐day fermentation could be an optimal process for excavation of applying wampee leaves into food and wine industries.

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“…The phloretin stock solution was diluted in distilled H 2 O to 4 μg/mL and 40 μg/mL for the low and high ligand treatments, respectively. Vanillic acid (H36001, Sigma-Aldrich), with a 7 min half-life (Yrbas et al 2015), was prepared as a stock solution at 1,680 μg/mL by dissolving the powder in distilled H 2 O over a hot magnetic spin plate. Since vanillic acid is an acidic compound, 3 M of NaOH was added to neutralize the solution to a pH of 7.0 for fly food.…”

Section: Methodsmentioning

confidence: 99%

Spatial control of gene expression in flies using bacterially derived binary transactivation systems

Gamez¹,

Vesga

Méndez‐Sánchez

et al. 2020

Preprint

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Controlling gene expression is an instrumental tool for biotechnology, as it enables the dissection of gene function, affording precise spatial-temporal resolution. To generate this control, binary transactivational systems have been used employing a modular activator consisting of a DNA binding domain(s) fused to activation domain(s). For fly genetics, many binary transactivational systems have been exploited in vivo; however as the study of complex problems often requires multiple systems that can be used in parallel, there is a need to identify additional bipartite genetic systems. To expand this molecular genetic toolbox, we tested multiple bacterially-derived binary transactivational systems in Drosophila melanogaster including the p-CymR operon from Pseudomonas putida, PipR operon from Streptomyces coelicolor, TtgR operon from Pseudomonas putida, and the VanR operon from Caulobacter crescentus. Our work provides the first characterization of these systems in an animal model in vivo. For each system we demonstrate robust tissue-specific spatial transactivation of reporter gene expression, enabling future studies to exploit these transactivational systems for molecular genetic studies.

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Pharmacological mechanism underlying the antinociceptive activity of vanillic acid

Cited by 25 publications

References 32 publications

Bioactivities of Bruguiera gymnorrhiza and profiling of its bioactive polyphenols by HPLC-DAD

Bioactivities of Bruguiera gymnorrhiza and profiling of its bioactive polyphenols by HPLC-DAD

Dynamic effects of fermentation on phytochemical composition and antioxidant properties of wampee (Clausena lansium (Lour.) Skeel) leaves

Spatial control of gene expression in flies using bacterially derived binary transactivation systems

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