Pharmacological Potential of Marine Microbes

Pandey, Abhishek

doi:10.1007/978-3-030-04675-0_1

Cited by 6 publications

(3 citation statements)

References 92 publications

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“…Besides bacteria, other marine microorganisms, such as endophytic fungi, are known to produce several types of cytotoxic compounds and have been sourced from several different environments. Some unique cytotoxic compounds, like the first methylthio-substituted aspochalasin derivative, active against prostate and colorectal cancer cell lines, was isolated from an Aspergillus strain recovered from the marine isopod Ligia oceanica [64]. Furthermore, the sponge-associated Penicillium chrysogenum produces the alkaloid sorbicillactone A, a potential active compound against leukemia cells.…”

Section: Plos Onementioning

confidence: 99%

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Metabolomic study of marine Streptomyces sp.: Secondary metabolites and the production of potential anticancer compounds

et al. 2020

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Resorting to a One Strain Many Compounds (OSMAC) approach, the marine Streptomyces sp. BRB081 strain was grown in six different media settings over 1, 2, 3 or 7 days. Extractions of mycelium and broth were conducted separately for each media and cultivation period by sonication using methanol/acetone 1:1 and agitation with ethyl acetate, respectively. All methanol/acetone and ethyl acetate crude extracts were analysed by HPLC-MS/MS and data treatment was performed through GNPS platform using MZmine 2 software. In parallel, the genome was sequenced, assembled and mined to search for biosynthetic gene clusters (BGC) of secondary metabolites using the AntiSMASH 5.0 software. Spectral library search tool allowed the annotation of desferrioxamines, fatty acid amides, diketopiperazines, xanthurenic acid and, remarkably, the cyclic octapeptides surugamides. Molecular network analysis allowed the observation of the surugamides cluster, where surugamide A and the protonated molecule corresponding to the B-E isomers, as well as two potentially new analogues, were detected. Data treatment through MZmine 2 software allowed to distinguish that the largest amount of surugamides was obtained by cultivating BRB081 in SCB medium during 7 days and extraction of culture broth. Using the same data treatment, a chemical barcode was created for easy visualization and comparison of the metabolites produced overtime in all media. By genome mining of BRB081 four regions of biosynthetic gene clusters of secondary metabolites were detected supporting the metabolic data. Cytotoxic evaluation of all crude extracts using MTT assay revealed the highest bioactivity was also observed for extracts obtained in the optimal conditions as those for surugamides production, suggesting these to be the main active compounds herein. This method allowed the identification of compounds in the crude extracts and guided the selection of best conditions for production of bioactive compounds.

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Section: Plos Onementioning

confidence: 99%

“…This compound is a cyclic dipeptide analog of halimide, a DKP isolated from Aspergillus ustus found in association with green algae Halimeda sp. [64].…”

Section: Plos Onementioning

confidence: 99%

Metabolomic study of marine Streptomyces sp.: Secondary metabolites and the production of potential anticancer compounds

et al. 2020

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“…Marine microbes, for example, have gained the attention of many researchers due to the chemical and structural diversity of the metabolites they produce and the various modes of action they exhibit [2]. Drug candidates that were purified and characterized from marine microbial symbionts showed potent bioactivities against various pathogens and diseases, including antibiotic-resistant micro-organisms, diabetes, cancer and inflammation [2][3][4][5]. However, the potential use of marine resources in developing new pharmaceuticals is still underexplored and presents a new area for natural product research.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and quorum sensing inhibitory activity of epiphytic bacteria isolated from the red alga Halymenia durvillei

Padayao,

Patalinghug

et al. 2023

Access Microbiology

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Halymenia durvillei is a red alga that is commonly utilized in the Philippines as food and as a source of high-value natural products for industrial applications. However, there are no studies regarding the microbial community associated with H. durvillei and its potential applications. This study aimed to isolate and identify the epiphytic bacteria of H. durvillei and determine their antimicrobial and quorum sensing inhibitory (QSI) effects. The thalli of H. durvillei were collected at the shores of Santa Fe, Bantayan, Cebu, Philippines. Bacterial isolates were identified using 16S rRNA, and their ethyl acetate (EtOAc) extracts were subjected to antimicrobial susceptibility tests against representative species of yeast and Gram-negative and Gram-positive bacteria. Their QSI activity against Chromobacterium violaceum was also determined. Fourteen distinct bacterial colonies belonging to four genera, namely Alteromonas (3), Bacillus (5), Oceanobacillus (1) and Vibrio (5), were successfully isolated and identified. All 14 bacterial isolates exhibited antibacterial effects. EPB9, identified as Bacillus safensis , consistently showed the strongest inhibition against Escherichia coli , Staphylococcus aureus and Staphylococcus epidermidis , with minimum inhibitory concentrations (MICs) ranging from 0.0625 to 1.0 mg ml−1. In contrast, all 14 isolates showed weak antifungal effects. Both B. safensis (EPB9) and Bacillus australimaris (EPB15) exhibited QSI effects at 100 mg ml−1, showing opaque zones of 3.1±0.9 and 3.8±0.4 mm, respectively. This study is the first to isolate and identify the distinct microbial epiphytic bacterial community of H. durvillei and its potential as an abundant resource for new antibacterial and QSI bioactives.

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Marine macroalga-associated heterotroph Bacillus velezensis as prospective therapeutic agent

Francis

Chakraborty

2021

Arch Microbiol

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Pharmacological Potential of Marine Microbes

Cited by 6 publications

References 92 publications

Metabolomic study of marine Streptomyces sp.: Secondary metabolites and the production of potential anticancer compounds

Metabolomic study of marine Streptomyces sp.: Secondary metabolites and the production of potential anticancer compounds

Antimicrobial and quorum sensing inhibitory activity of epiphytic bacteria isolated from the red alga Halymenia durvillei

Marine macroalga-associated heterotroph Bacillus velezensis as prospective therapeutic agent

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