Pharmacological studies on antidiarrheal effects of berberine and Geranii Herba.

Takase, Hideki; Yamamoto, Kazunori; Ito, Keizo; Yumioka, Eizaburou

doi:10.1254/fpj.102.101

Cited by 26 publications

(14 citation statements)

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“…Berberine has a wide range of pharmacological and biochemical effects. It has been reported that berberine can be used as an anti-diarrhea, antiarrhythmia and anti-inflammatory agent (6)(7)(8). Additionally, berberine has also been shown to have antitumor effects on many cancer cell lines, including leucocytes, liver, lung, stomach, colon, skin, oral, esophageal, brain, bone, breast and genital cancer cells (9)(10)(11)(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

Radiosensitization effects of berberine on human breast cancer cells

Wang

Yang

2012

International Journal of Molecular Medicine

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Berberine, an isoquinoline derivative alkaloid, has recently been shown to have antitumor activity. The present study aimed to investigate the effects of the concomitant administration of berberine and radiation on breast cancer. The effects of berberine on the radiosensitivity of MCF-7 and MDA-MB-468 cells were evaluated by using cell clonogenic assays. Cells pre-treated with berberine or dimethyl sulfoxide (DMSO) for 24 h were irradiated using a Faxitron Cabinet X-ray System to deliver the indicated doses (0, 1, 2, 3 and 4 Gy). Changes in cell cycle distribution were determined by flow cytometry. γ-H2AX foci were detected by immunofluorescence staining. The levels of Ku70, Ku86 and RAD51 proteins were evaluated by western blot analysis. We observed that berberine increased the MCF-7 and MDA-MB-468 cell radiosensitivity with cell clonogenic assays. the radiation-induced G2/M cell cycle delay was reduced in the MCF-7 cells pre-teated with berberine. Berberine pre-treatment prolonged the persistence of DNA double-strand breaks in the MCF-7 cell line. In comparison with the control cells, the protein levels of RAD51 were decreased in the MCF-7 and MDA-MB-468 cells treated with berberine, and in the cells pre-treated with 15 µM berberine for 24 h, the level of RAD51 protein decreased significantly at the indicated time-points (0, 2, 6 and 24 h) following X-ray exposure. In conclusion, berberine sensitizes human breast cancer cells to ionizing radiation by inducing cell cycle arrest and the downregulation of the homologous recombination repair protein, RAD51. Berberine may be a promising radiosensitizer for the treatment of breast cancer.

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Section: Introductionmentioning

confidence: 99%

Radiosensitization effects of berberine on human breast cancer cells

Wang

Yang

2012

International Journal of Molecular Medicine

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“…1) isolated from Hydrastis canadensis, Coptis chinensis, Berberis aquifolium, and Berberis vulgaris, has been extensively studied on its multiple biological and pharmacological activities [Creasy, 1997;Schmeller et al, 1997;Kuo et al, 2004]. It has been reported that BBR can be used as an anti-diarrhea, anti-hypertension, anti-arrhythmia, and antiinflammatory agent [Tai et al, 1981;Huang et al, 1989;Takase et al, 1993;Yamamoto et al, 1993;Fukuda et al, 1999;Lau et al, 2001]. Additionally, the natural product was demonstrated to possess an anti-tumor activity [Chi et al, 1994;Kuo et al, 1995;Miura et al, 1997;Chung et al, 2000;Iizuka et al, 2000;Hwang et al, 2006;Lin et al, 2006].…”

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confidence: 99%

The combination of berberine and irradiation enhances anti‐cancer effects via activation of p38 MAPK pathway and ROS generation in human hepatoma cells

Hur

Hyun

Lim

et al. 2009

J of Cellular Biochemistry

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Berberine, an isoquinoline plant alkaloid, has been known to generate a wide variety of biochemical and pharmacological effects. In order to elucidate the molecular mechanism for the berberine-induced enhancement of radio-sensitization, the human hepatoma HepG2 cells were treated with berberine combined with irradiation. The anti-tumor effect of gamma radiation was found to be significantly enhanced by berberine. The evidences of apoptosis, such as apoptotic DNA fragmentation and annexin V staining, were observed in the cells treated with the combination of berberine and irradiation. Additionally, the levels of reactive oxygen species (ROS) and nitric oxide (NO) were apparently elevated in the combination system. The activations of p38, Bax, and caspase-3 were also detected in the irradiated cells pretreated with berberine. The productions of ROS and annexin V staining in the cells treated with the combination of berberine and irradiation were significantly inhibited by the specific inhibitor of p38 MAPK, SB203580. The cell death induced by berberine alone or the combination of berberine and irradiation was suppressed by the anti-oxidant, N-acetyl cysteine (NAC). Taken together, the present results clearly indicate that the combination of berberine and gamma-radiation enhance the anti-cancer effects through the p38 MAPK pathway and ROS generation.

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“…5 cells/well of C6 cells were grown in a 12-well plate in KMHF culture medium for 24 h then treated individually with 0, 50, 100 and 200 μM berberine and cells incubated at 37˚C, 5% CO 2 and 95% air for 48 h. Cells were harvested from each sample for 4,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining, as described previously (9). After staining, the cells were examined and were photographed under a fluorescence microscope (20,21).…”

Section: Dapi Staining For Apoptosis Approximately 2x10mentioning

confidence: 99%

“…Berberine, an isoquinoline alkaloid, is present in the roots, rhizome and stem bark of Berberis aquifolium, Berberis vulgaris and Berberis aristata (7). Berberine has been reported to exhibit a wide range of biological activities such as anti-diarrheal, anti-arrhythmic and anti-tumor activities (7)(8)(9)(10). Berberine also inhibits cyclooxygenase-2 transcriptional activity in human colon cancer cells (11,12), inhibits tumor promoting activity of teleocidin in two-stage chemical carcinogenesis on mouse skin (13), inhibits DNA topoisomerase I and II in a biochemical system (14,15) and induces cell cycle arrest and apoptosis in human epidermoid carcinoma A431 cells (16).…”

Section: Introductionmentioning

confidence: 99%

Involvement of reactive oxygen species and caspase-dependent pathway in berberine-induced cell cycle arrest and apoptosis in C6 rat glioma cells

et al. 2009

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Abstract. The cytotoxicity of berberine on C6 rat glioma cells indicated that berberine induced morphological changes and caused cell death through G2/M arrest and apoptosis. While undergoing apoptosis, there was a remarkable accumulation of G2/M cells with the upregulatoin of Wee1 but it also inhibited cyclin B, CDK1 and Cdc25c that led to G2/M arrest. Along with cytotoxicity in C6 cells, several apoptotic events including mitochondrial cytochrome c release, activation of caspase-9, -3 and -8 and DNA fragmentation were induced. Berberine increased the levels of GADD153 and GRP 78 in C6 cells based on the examination of Western blotting and this is a major hallmark of endoplasmic reticulum (ER) stress. We also found that berberine promoted the production of reactive oxygen species and Ca 2+ in C6 cells. Western blotting assay also showed that berberine inhibited the levels of anti-apoptotic protein Bcl-2 but increased the levels of pro-apoptotic protein Bax before leading to a decrease in the levels of mitochondrial membrane potential (Δae m ) followed by cytochrome c release that caused the activations of capase-9 and -3 for apoptotic occurrence. The caspase-8, -9 and -3 were activated by berberine in C6 cells based on the substrate solution (PhiPhiLux-G 1 D 1 , CaspaLux 8-L 1 D 2 , CaspaLux 9-M 1 D 2 for caspase-3, -8 and -9, respectively) and analyzed by flow cytometer and each inhibitor of caspase-8, -9 and -3 led to increase the percentage of viable C6 cells after exposure to berberine. This finding was also confirmed by Western blot assay which showed that berberine promoted the active form of caspase-8, -9 and -3. These results demonstrate that the cytotoxicity of berberine in C6 rat glioma cells is attributable to apoptosis mainly through induced G2/M-arrested cells, in an ER-dependent manner, via a mitochondria-dependent caspase pathway regulated by Bax and Bcl-2.

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Pharmacological studies on antidiarrheal effects of berberine and Geranii Herba.

Cited by 26 publications

References 0 publications

Radiosensitization effects of berberine on human breast cancer cells

Radiosensitization effects of berberine on human breast cancer cells

The combination of berberine and irradiation enhances anti‐cancer effects via activation of p38 MAPK pathway and ROS generation in human hepatoma cells

Involvement of reactive oxygen species and caspase-dependent pathway in berberine-induced cell cycle arrest and apoptosis in C6 rat glioma cells

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