Pharmacological studies on factors influencing the collecting phase of the cystometrogram in urethane‐anesthetized rats

Abstract

Maggi, C.A., P. Santicioli, and A. Meli: Pharmacological studies on factors influencing the collecting phase of cystometrogram in urethane-anesthetized rats. Drug Dev. Res. The effect of various pharmacological interventions on the collecting phase of cystometrogram (CMG) has been investigated by means of a new cystometric procedure in urethaneanesthetized rats. The method involves the transvesical infusion, through a needle inserted in the bladder dome, of warm (37OC) saline at a rate (2.8 mllh) simulating a maximal hourly diuresis value for this species. In these conditions bladder voiding is produced through the activation of a supraspinal vesico-vesical reflex. Either chemical (6-hydroxydopamine, reserpine) or surgical (section of hypogastric nerves) sympathectomy produced a picture of detrusor hyperreflexia and urine dripping, mimicking cystometric finding of human disease. This new procedure may be useful for screening and development of new drugs for treatment of urinary incontinence.

Functional role of M2 and M3 muscarinic receptors in the urinary bladder of rats in vitro and in vivo

“…Animal studies have shown that the sympathetic input to the bladder is tonically active during filling (Edwardsen, 1968; de Groat & Lalley, 1972; Satchell & Vaughan, 1988). Furthermore, hypo‐gastric nerve section, ganglionic blockade and β‐adrenoceptor blockade produce decreases in bladder compliance, bladder capacity and volume threshold for micturition, consistent with a sympathoinhibitory tone to the bladder during filling (Maggi et al , 1987; Flood et al , 1988; Vaughan & Satchell, 1992). It has recently been proposed that the sympathoinhibitory drive to the bladder partially reduces the level of bladder wall tension transduced by the bladder wall mechanoreceptors, and thus delays the time at which micturition threshold is attained (Vaughan & Satchell, 1995).…”

Bladder Function in the Aged Rat: A Functional and Morphological Study

“…Through a midline inci sion of the abdomen, the urinary bladder was exposed and the vesical dome apex was cannulated. This method permits to record storage and voiding phases of the bladder [8]. The catheter was connected both to an infusion pump and a pressure transducer.…”

Effect of an Atypical Adrenergic .BETA.3-Agonist, GS-332: Sodium (2R)- (3- (3- (2-(3-Chlorophenyl)-2-Hydroxyethylamino) Cyclohexyl) Phenoxy) Acetate, on Urinary Bladder Function in Rats.

“…Effects of GS-332 on micturition intervals and micturition reflex in anesthetized rat cystometory Experiment was performed according to the method of Maggi et al, 1987 (Japan). GS-332 and clenbuterol were dissolved in distilled water, and SR59230A was dissolved in DMSO.…”