Pharmacology of Botulinum Neurotoxins: Exploitation of Their Multifunctional Activities as Transmitter Release Inhibitors and Neuron-Targeted Delivery Vehicles

Engineering of botulinum neurotoxins as novel therapeutic tools

“…In this regard, BoNT/E was evaluated because of its deletion of 26 C-terminal residues from SNAP-25 rather than 9 amino acids cleaved off by /A. Moreover, elevation of intraneuronal [Ca 2+ ] can temporarily overcome /A-but not /E-induced blockade of acetylcholine release/neuromuscular transmission [50] and reviewed in [51]. Surprisingly, /E virtually failed to cleave SNAP-25 and block CGRP release from TGNs triggered by any stimulus (Figure 34.8B).…”