Pharyngeal carriage of group B streptococci: detection by three methods

Ferrieri, Patricia; Blair, Laura L.

doi:10.1128/jcm.6.2.136-139.1977

Cited by 42 publications

(1 citation statement)

References 12 publications

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“…DMPA0115) A variety of fastidious bacteria [ 106 ] Blood agar with nalidixic acid and colistin (DMP/NICD, South Africa; cat no. DMPA0110) Gram-positive bacteria [ 107 ] Chapman’s agar (or mannitol salt agar) (DMP/NICD, South Africa; cat no. DMPA0316) Gram-positive bacteria, specifically staphylococci [ 108 ] Brain–Heart Infusion (BHI) agar (DMP/NICD, South Africa; cat no.…”

Section: Methodsmentioning

confidence: 99%

Microbiota identified from preserved Anopheles

Silva

Matsena-Zingoni

Koekemoer

et al. 2021

Malar J

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Background Mosquito species from the Anopheles gambiae complex and the Anopheles funestus group are dominant African malaria vectors. Mosquito microbiota play vital roles in physiology and vector competence. Recent research has focused on investigating the mosquito microbiota, especially in wild populations. Wild mosquitoes are preserved and transported to a laboratory for analyses. Thus far, microbial characterization post-preservation has been investigated in only Aedes vexans and Culex pipiens. Investigating the efficacy of cost-effective preservatives has also been limited to AllProtect reagent, ethanol and nucleic acid preservation buffer. This study characterized the microbiota of African Anopheles vectors: Anopheles arabiensis (member of the An. gambiae complex) and An. funestus (member of the An. funestus group), preserved on silica desiccant and RNAlater® solution. Methods Microbial composition and diversity were characterized using culture-dependent (midgut dissections, culturomics, MALDI-TOF MS) and culture-independent techniques (abdominal dissections, DNA extraction, next-generation sequencing) from laboratory (colonized) and field-collected mosquitoes. Colonized mosquitoes were either fresh (non-preserved) or preserved for 4 and 12 weeks on silica or in RNAlater®. Microbiota were also characterized from field-collected An. arabiensis preserved on silica for 8, 12 and 16 weeks. Results Elizabethkingia anophelis and Serratia oryzae were common between both vector species, while Enterobacter cloacae and Staphylococcus epidermidis were specific to females and males, respectively. Microbial diversity was not influenced by sex, condition (fresh or preserved), preservative, or preservation time-period; however, the type of bacterial identification technique affected all microbial diversity indices. Conclusions This study broadly characterized the microbiota of An. arabiensis and An. funestus. Silica- and RNAlater®-preservation were appropriate when paired with culture-dependent and culture-independent techniques, respectively. These results broaden the selection of cost-effective methods available for handling vector samples for downstream microbial analyses.

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Section: Methodsmentioning

confidence: 99%

Microbiota identified from preserved Anopheles

Silva

Matsena-Zingoni

Koekemoer

et al. 2021

Malar J

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β-Hemolytic Streptococcus

Breese¹

1978

Am J Dis Child

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The Journal is privileged to publish the following abridged chapter from a forthcoming book on streptococcal infection. Dr Breese has spent a lifetime of study on this subject. His experiences gained during the course ofprivate practice should be ofinterest to all of us. A second abridged chapter will appear in the June issue.\p=m-\Ed To be published in Beta Hemolytic Streptococcal Diseases by Burtis B. Breese, MD, and Caroline B. Hall, MD.

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Association of Group C β-Hemolytic Streptococci With Endemic Pharyngitis Among College Students

Turner¹

1990

JAMA

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Throat cultures were performed throughout 2 school years to determine whether non-group A beta-hemolytic streptococci (NGA BHS) could be isolated more frequently in 232 college students who had symptomatic pharyngitis than from 198 age-matched controls with noninfectious problems. Duplicate throat swabs were inoculated onto plates that contained sheep blood agar, one plate being incubated in a 5% CO2 atmosphere and the other in an anaerobic environment. The BHS were grouped using latex agglutination. Among the NGA BHS, only those from group C were isolated significantly more often among the patients compared with the controls (26% vs 11%). Quantitative colony counts of isolates of group C BHS were generally higher among patients than controls. Patients with group C BHS had fever, exudative tonsillitis, and anterior cervical adenopathy significantly more frequently than did patients who had throat cultures that were negative for group C BHS. Group C BHS were epidemiologically associated with endemic pharyngitis in this college student population.

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Pharyngeal carriage of group B streptococci: detection by three methods

Cited by 42 publications

References 12 publications

Microbiota identified from preserved Anopheles

Microbiota identified from preserved Anopheles

β-Hemolytic Streptococcus

Association of Group C β-Hemolytic Streptococci With Endemic Pharyngitis Among College Students

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