Phase behavior of binary bilayer membrane of dipalmitoylphosphatidylcholine and stigmasterol

Tamai, Naoto; Inazawa, Sanae; Takeuchi, Shin; Goto, Masaki; Matsuki, Hitoshi

doi:10.1007/s10973-018-7597-9

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…It is a well-known fact that there is a link between ∆T 1/2 and both the cooperativity of a transition and the cooperativity of the main transition of DPPC bilayer, i.e., the former is inversely related to the latter. This is a key point considering how fast or how far the results of a trans-gauche conformational change will propagate within the membrane when one constituent phospholipid molecule underwent the conformational change [ 17 ]. Thus, the width of the main peak is related to the cooperativity of the phase transition, and the increasing presence of 1-ANISA-2-PA-PC and 1-PA-2-ANISA-PC significantly decreased the cooperativity.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the Physico-Chemical Properties of Liposomes Assembled from Bioconjugates of Anisic Acid with Phosphatidylcholine

Pruchnik

Gliszczyńska

Włoch

2021

IJMS

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The aim of this work was the evaluation of the physico-chemical properties of a new type of liposomes that are composed of DPPC and bioconjugates of anisic acid with phosphatidylcholine. In particular, the impact of modified anisic acid phospholipids on the thermotropic parameters of liposomes was determined, which is crucial for using them as potential carriers of active substances in cancer therapies. Their properties were determined using three biophysical methods, namely differential scanning calorimetry (DSC), steady-state fluorimetry and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). Moreover, temperature studies of liposomes composed of DPPC and bioconjugates of anisic acid with phosphatidylcholine provided information about the phase transition, fluidity regarding chain order, hydration and dynamics. The DSC results show that the main phase transition peak for conjugates of anisic acid with phosphatidylcholine molecules was broadened and shifted to a lower temperature in a concentration- and structure-dependent manner. The ATR-FTIR results and the results of measurements conducted using fluorescent probes located at different regions in the lipid bilayer are in line with DSC. The results show that the new bioconjugates with phosphatidylcholine have a significant impact on the physico-chemical properties of a membrane and cause a decrease in the temperature of the main phase transition. The consequence of this is greater fluidity of the lipid bilayer.

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Section: Resultsmentioning

confidence: 99%

Evaluation of the Physico-Chemical Properties of Liposomes Assembled from Bioconjugates of Anisic Acid with Phosphatidylcholine

Pruchnik

Gliszczyńska

Włoch

2021

IJMS

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“…1) Sterol effects on the phase transition temperature of DPPC and on the solubilization of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine by Triton X-100 demonstrate that cholesterol, compared with the plant sterols such as β-sitosterol and stigmasterol, interacts more favorably with phospholipids. 28) Thus, cholesterol leads to the stronger phospholipid condensation than the plant sterols, 11) suggesting that the bulky ethyl group at C-24 in plant sterols may disturb a tight packing of sterol side chain with the hydrocarbon chains of phospholipids. 29) Therefore, such a disturbance may contribute to the enhancement of hemolysis under pressure in plant sterol-treated erythrocytes.…”

Section: Discussionmentioning

confidence: 99%

Importance of Cholesterol Side Chain in the Membrane Stability of Human Erythrocytes

Yamaguchi

Manaka

Ogura

et al. 2021

Biological & Pharmaceutical Bulletin

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Cholesterol suppresses the hemolysis and the detachment of cytoskeletal proteins from bilayer in the human erythrocyte membrane under stress conditions. However, there is little information on how cholesterol functions. So, examining the role of a short side chain of cholesterol, we used the plant sterols such as β-sitosterol and stigmasterol. Incorporation of sterols into the membrane using a sterol/methyl-βcyclodextrin complex was confirmed by the mass spectrometry. Hemolysis of human erythrocytes under high hydrostatic pressure (200 MPa) or hypotonic conditions was suppressed by cholesterol, but not by β-sitosterol and stigmasterol. Moreover, the bilayer-cytoskeleton interaction was also strengthened by cholesterol, but not by β-sitosterol and stigmasterol. Taken together, we suggest that the short side chain of cholesterol plays an important role in the membrane stability of human erythrocytes.

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DSC studies on the interaction of lipophilic cytarabine prodrugs with DMPC multilamellar vesicles

Escobar

Fernández

Giordani

et al. 2019

J Therm Anal Calorim

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Phase behavior of binary bilayer membrane of dipalmitoylphosphatidylcholine and stigmasterol

Cited by 4 publications

References 33 publications

Evaluation of the Physico-Chemical Properties of Liposomes Assembled from Bioconjugates of Anisic Acid with Phosphatidylcholine

Evaluation of the Physico-Chemical Properties of Liposomes Assembled from Bioconjugates of Anisic Acid with Phosphatidylcholine

Importance of Cholesterol Side Chain in the Membrane Stability of Human Erythrocytes

DSC studies on the interaction of lipophilic cytarabine prodrugs with DMPC multilamellar vesicles

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