Phase changes of cardiolipin vesicles mediated by divalent cations

Vail, William J.; Stollery, John G.

doi:10.1016/0005-2736(79)90354-7

Cited by 70 publications

(34 citation statements)

References 7 publications

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“…The bilayer-, hexagonal Ht~ phase transition can be readily reversed by dialysis of the Cacardiolipin (l: l) complex against EDTA containing buffers resulting in the formation of large bilayer vesicles [9,10]. Our experiments show that in the absence of EDTA not only the rate of this process is extremely slow but that also several structural changes occur.…”

Section: Discussionmentioning

confidence: 71%

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Further aspects of the Ca2+-dependent polymorphism of bovine heart cardiolipin

Kruijff

Verkleij

Leunissen‐Bijvelt

et al. 1982

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Section: Discussionmentioning

confidence: 71%

“…In excess buffer, the sodium salt of bovine heart cardiolipin is organized in extended bilayers [8][9][10]. Under similar conditions the Ca, Mg, Mn and Ba salts, dependent upon the temperature, can form the lamellar and hexagonal H n phase [8,11,12].…”

Section: Introductionmentioning

confidence: 99%

Further aspects of the Ca2+-dependent polymorphism of bovine heart cardiolipin

Kruijff

Verkleij

Leunissen‐Bijvelt

et al. 1982

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…Such structures appear to occur as intermediaries in the bilayer to hexagonal (HII) phase transitions observed for cardiolipin on addition of Ca :÷ [6,11 ]. We therefore consider that the fusion of vesicular model membrane systems composed of cardiolipin and egg yolk phosphatidylcholine arises from the ability of the endogeneous cardiolipin to facilitate formation of non-bilayer lipid structures in the presence of Ca 2÷.…”

Section: Discussionmentioning

confidence: 99%

Fusion of phospholipid vesicles in association with the appearance of lipidic particles as visualized by freeze fracturing

Verkleij

Mombers

Gerritsen

et al. 1979

Biochimica et Biophysica Acta (BBA) - Biomembranes

166

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SummaryThe addition of Ca 2÷ to small unilamellar vesicles of an equimolar mixture of egg phosphatidylcholine and cardiolipin induces fusion of these vesicles in association with the appearance of lipidic particles on the fusion sites.Membrane fusion clearly requires that participating lipids adopt transitory non-bilayer configurations during the intermediate stages. The nature of the possible intermediate structures (such as micellar [1] or inverted micellar [2,3] ) and their relation to the physical properties of membrane lipids remain, however, a matter of some speculation. Recently, it has been argued that endogenous lipids which preferentially adopt the hexagonal (HII) phase under certain conditions may be directly involved in fusion events [4]. In this regard it has been established that the presence of Ca 2÷ is vital to the fusion process [ 5] and that the addition of Ca 2÷ can trigger formation of the hexagonal (HII) phase in model membrane systems consisting of certain pure [6] or mixed [7] species of naturally occurring phospholipid. It is therefore of interest to first establish that Ca 2÷ can also induce fusion between model systems partly comprised of such phospholipids, and secondly to establish that such fusion events proceed via the non-bilayer structures engendered by the presence of Ca 2.. In this work we present results obtained employing model systems comprised of an equimolar mixture of bovine heart cardiolipin and egg yolk phosphatidylcholine in conjunction with

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“…This transition temperature is strongly dependent on the fatty-acyl chain composition. Saturated species can only form a bilayer, while egg phosphatidylethanolamine adopts the hexagonal HH phase at about 25°C and dioleoylphosphatidylethanolamine at about 5 ° C. Other membrane lipids like cardiolipin [4,6,7] and monoglucosyldiacylglycerol [8,9] also adopt the hexagonal H H phase at physiological temperature. Most interestingly, in mixtures of these lipids with phosphatid choline, lipidic particles of an inverted micellar nature associated with the bilayer have been observed both by freeze-fracture electron microscopy and by 31P-NMR [9,10].…”

Section: Discussionmentioning

confidence: 99%

A possible role of rhodopsin in maintaining bilayer structure in the photoreceptor membrane

Grip

Drenthe

Echteld

et al. 1979

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Summary31p-NMR measurements demonstrate that at 37 ° C, independent of the photolytic state of the photopigment rhodopsin, the lipids in the photoreceptormembrane are almost exclusively organised in a bilayer. In strong contrast, the 31P-NMR spectra of the extracted lipids are characteristic for the hexagonal HII phase and an isotropic phase. The isotropic phase is characterised by freeze-fracture electron microscopy as particles and pits on smooth surfaces, possibly indicating inverted micelles. These results suggest a structural role for rhodopsin in maintaining the photoreceptor membrane lipids in a bilayer configuration.It has recently been demonstrated that the lipid bilayer, while still the basic feature of biological membranes, is not the only conformational state in which the lipids in membranes can occur. 31P-NMR studies have presented strong evidence that in the endoplasmic reticulum of rat, bovine and rabbit liver the phospholipids can undergo isotropic motion, which suggests that inverted micellar structures may be present in conjunction with the bilayer [ 1 ]. Model studies of water-phospholipid mixtures show that the preferred phase depends on the type of lipid [ 2--6 ]. Under most conditions the thermodynamically most stable phase of phosphatidylcholine is the bilayer. Unsaturated phosphatidylethanolamine also prefers the bilayer conformation at lower temperatures, but at increasing temperature it undergoes a phase Abbreviation: Mops, 3-(N-morpholino)propanesulfonic acid.

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Phase changes of cardiolipin vesicles mediated by divalent cations

Cited by 70 publications

References 7 publications

Further aspects of the Ca2+-dependent polymorphism of bovine heart cardiolipin

Further aspects of the Ca2+-dependent polymorphism of bovine heart cardiolipin

Fusion of phospholipid vesicles in association with the appearance of lipidic particles as visualized by freeze fracturing

A possible role of rhodopsin in maintaining bilayer structure in the photoreceptor membrane

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