Phase-dependent Changes of DNA, RNA and Protein Accumulation during the Ontogenesis of Broad Bean Seeds (Vicia faba L., var. minor)

Manteuffel, Renate; Müntz, Klaus; Püchel, Manfred; Scholz, Günter

doi:10.1016/s0015-3796(17)30944-7

Cited by 26 publications

(6 citation statements)

References 26 publications

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“…Figure 3A shows that SUCS m R N A was already present at 15 DAF when embryo cells are still rapidly dividing (see Manteuffel et al 1976). The level increased to a broad maximum around (Chopra et al 1992) and Oryza sativa ) genomic sequences.…”

Section: Levels Of Sucs Mrna Change During Cotyledon Developmentmentioning

confidence: 99%

A sucrose-synthase gene of Vicia faba L.: Expression pattern in developing seeds in relation to starch synthesis and metabolic regulation

Heim

Weber

Bäumlein

et al. 1993

Planta

182

146

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Copy-DNA clones encoding a single class of sucrose-synthase (SUCS; EC 2.4.1.13) subunit have been isolated and sequenced from a Vicia faba L. seed cotyledonary library. Southern analyses indicated the existence of only one gene. Transcript levels determined by Northern blot hybridisation steadily increased until the middle of development [25-35 days after flowering (DAF)] and declined thereafter. Sucrose levels approximately paralleled levels of SUCS mRNA. The activity of SUCS increased with decreasing fructose and glucose concentrations and peaked about 10 d later than mRNA levels. In-vitro culture experiments demonstrated that increasing the sucrose concentration leads to increased levels of SUCS mRNA. The SUCS mRNA was also synthesised in seed-coat tissue, but in lower amounts than in cotyledons and with a different developmental profile. The early peak level of SUCS mRNA (20 DAF) in seed coats coincided with the peak in the amount of sucrose and with a peak of transiently synthesised starch.

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Section: Levels Of Sucs Mrna Change During Cotyledon Developmentmentioning

confidence: 99%

A sucrose-synthase gene of Vicia faba L.: Expression pattern in developing seeds in relation to starch synthesis and metabolic regulation

Heim

Weber

Bäumlein

et al. 1993

Planta

182

146

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“…In cotyledons of ripening (and endopolyploid) Vicia faba (Fabaceae) seeds even the ratio DNA:RNA remains almost constant. (87) Therefore, a simple enhancement of functional activity of a cell by raising the number of DNA templates per cell volume seems not to exist. A change of genomic ploidy (e.g.…”

Section: Ethylenementioning

confidence: 99%

Endopolyploidy in seed plants

Barow¹

2006

Bioessays

169

149

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Two main attempts have been suggested for the biological significance of endopolyploidy: (i) provision of high DNA amounts to support high synthetic demands in certain cells and (ii) compensation for a lack of nuclear DNA in species with small genomes. However, in seed plants, the positive correlation between DNA content and cell volume of endopolyploid cells suggests other possibilities. Cell size paralleled by the endopolyploidy level has an impact on growth and development. Endopolyploidy levels in turn are characteristic for a given species and even families, reflecting the adaptation to certain habitats during phylogeny. Furthermore, endopolyploidy levels vary to some degree between individuals of one species in response to different environmental conditions. In addition, endopolyploidy differs between different tissues suggests that a certain cell size is advantageous for a given cell function. This article reviews these findings and discusses more conclusive possible functions of endopolyploidy.

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“…A and B, after Brandt (1976); C, after Manteuffel et al (1976); and D, after Gayler and Sykes (1981).…”

Section: Cerealsmentioning

confidence: 99%

Seeds

Bewley

Black

1994

1,480

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Phase-dependent Changes of DNA, RNA and Protein Accumulation during the Ontogenesis of Broad Bean Seeds (Vicia faba L., var. minor)

Cited by 26 publications

References 26 publications

A sucrose-synthase gene of Vicia faba L.: Expression pattern in developing seeds in relation to starch synthesis and metabolic regulation

A sucrose-synthase gene of Vicia faba L.: Expression pattern in developing seeds in relation to starch synthesis and metabolic regulation

Endopolyploidy in seed plants

Seeds

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