Phase Diagram of Collective Motion of Bacterial Cells in a Shallow Circular Pool

Wakita, Jun–ichi; Tsukamoto, Shota; Yamamoto, Ken; Katori, Makoto; Yamada, Yasuyuki

doi:10.7566/jpsj.84.124001

Cited by 10 publications

(12 citation statements)

References 34 publications

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“…The net vertical force integrated over the circle is 0.79 mN, which is nearly 300 times larger than the bead's weight. The gel deformation under this capillary force amounts to $2 mm, comparable to what was observed in (37). This deformation also accounts for our observation that glass beads or graphite particles anchor firmly to the surface once the shallow water tents are established.…”

Section: Comparison and Contrast With A Similar Recent Studysupporting

confidence: 86%

“…Wakita et al (37) investigated bacterial motion in a shallow circular pool formed by compression and removal of glass FIGURE 5 Confocal microscopy images illustrating a small but notable compression of a 4.5 mm bead on a 0.5% agar surface. The surface is covered by much smaller beads of 0.5 mm diameter labeled in Dragon Green, which is excited by blue and green lights to emit green and red lights but with green the strongly favored emission.…”

Section: Comparison and Contrast With A Similar Recent Studymentioning

confidence: 99%

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Orbiting of Flagellated Bacteria within a Thin Fluid Film around Micrometer-Sized Particles

Araujo

Chen

Mani

et al. 2019

Biophysical Journal

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Bacterial motility under confinement is relevant to both environmental control and the spread of infection. Here, we report observations on Escherichia coli, Enterobacter sp., Pseudomonas aeruginosa, and Bacillus subtilis when they are confined within a thin layer of water around dispersed micrometer-sized particles sprinkled over a semisolid agar gel. In this setting, E. coli and Enterobacteria orbit around the dispersed particles. The liquid layer is shaped like a shallow tent with its height at the center set by the seeding particle, and the meniscus profile set by the strong surface tension of water. The tentshaped confinement and the left handedness of the flagellar filaments result in exclusively clockwise circular trajectories. The thin fluid layer is resilient because of a balance between evaporation and reinforcement of fluid that permeated out of the agar. The latter is driven by the Laplace pressure caused by the concave meniscus. In short, we explain the physical mechanism of a convenient method to entrap bacteria within localized thin fluid film near a permeable surface.

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Section: Comparison and Contrast With A Similar Recent Studysupporting

confidence: 86%

Section: Comparison and Contrast With A Similar Recent Studymentioning

confidence: 99%

Orbiting of Flagellated Bacteria within a Thin Fluid Film around Micrometer-Sized Particles

Araujo

Chen

Mani

et al. 2019

Biophysical Journal

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“…Bacterial colonies are formed through biological self-organization [1][2][3][4][5][6][7][8][9][10]. For example, pioneering studies on the morphology of Bacillus subtilis colonies demonstrated that colony morphology depends on the solidity and nutrient concentrations of the media [3,11].…”

Section: Introductionmentioning

confidence: 99%

Scattered migrating colony formation in the filamentous cyanobacterium, Pseudanabaena sp. NIES-4403

Yamamoto

Fukasawa

Shoji

et al. 2020

Preprint

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Background Bacteria have been reported to exhibit complicated morphological colony patterns on solid media, depending on intracellular, and extracellular factors such as motility, cell propagation, and cell-cell interaction. We isolated the filamentous cyanobacterium, Pseudanabaena sp. NIES-4403 (Pseudanabaena, hereafter), that forms scattered (discrete) migrating colonies on solid media. While the scattered colony pattern has been observed in some bacterial species, the mechanism underlying such a pattern still remains obscure. Results We studied the morphology of Pseudanabaena migrating collectively and found that this species forms randomly scattered clusters varying in size and further consists of a mixture of comet-like wandering clusters and rotating disks. Quantitative analysis of the formation of these wandering and rotating clusters showed that bacterial filaments tend to follow trajectories of previously migrating filaments at velocities that are dependent on filament length. Collisions between filaments occurred without crossing paths, which enhanced their nematic alignments, giving rise to bundle-like colonies. As cells increased and bundles aggregated, comet-like wandering clusters developed. The direction and velocity of the movement of cells in comet-like wandering clusters were highly coordinated. When the wandering clusters entered into a circular orbit, they turned into rotating disks, maintaining a more stable location. Disks may rotate for days, and the speed of cells within a rotating disk increases from the center to the outmost part of the disk. Using a minimal agent-based model, we reproduced some features of Pseudanabaena migrating clusters. Conclusion Based on these observations, we propose that Pseudanabaena forms scattered migrating colonies that undergo a series of transitions involving several morphological patterns. A minimal agent-based model is able to reconstruct some features of the observed migrating clusters.

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“…In a recent paper [16], we have reported the physical aspects of the collective motion of bacterial cells observed in shallow circular pools prepared on the surface of an agar plate. The diameters of the pools are arranged so as not to be much larger than the length of the bacterial cells swimming in the pools.…”

Section: Introductionmentioning

confidence: 99%

Self-Elongation with Sequential Folding of a Filament of Bacterial Cells

2015

Self Cite

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Under hard-agar and nutrient-rich conditions, a cell of Bacillus subtilis grows as a single filament owing to the failure of cell separation after each growth and division cycle. The self-elongating filament of cells shows sequential folding processes, and multifold structures extend over an agar plate. We report that the growth process from the exponential phase to the stationary phase is well described by the time evolution of fractal dimensions of the filament configuration. We propose a method of characterizing filament configurations using a set of lengths of multifold parts of a filament. Systems of differential equations are introduced to describe the folding processes that create multifold structures in the early stage of the growth process. We show that the fitting of experimental data to the solutions of equations is excellent, and the parameters involved in our model systems are determined.

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Phase Diagram of Collective Motion of Bacterial Cells in a Shallow Circular Pool

Cited by 10 publications

References 34 publications

Orbiting of Flagellated Bacteria within a Thin Fluid Film around Micrometer-Sized Particles

Orbiting of Flagellated Bacteria within a Thin Fluid Film around Micrometer-Sized Particles

Scattered migrating colony formation in the filamentous cyanobacterium, Pseudanabaena sp. NIES-4403

Self-Elongation with Sequential Folding of a Filament of Bacterial Cells

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