Phase diagrams of confined solutions of dimyristoylphosphatidylcholine (DMPC) lipid and cholesterol in nanotubes

Arai, Noriyoshi; Yasuoka, Kenji; Zeng, Xiao Cheng

doi:10.1007/s10404-012-1107-3

Cited by 11 publications

(10 citation statements)

References 45 publications

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“…Dissipative particle dynamics simulations of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) confined in hydrophilic pores suggest that cylindrical micelles can form in pores as small as 80% of the bilayer thickness of a lipid. [43] The simulations predict that isolated micelles can form in smaller pores and at smaller concentrations. The procedures used here may not have provided enough driving force to cause DPPC to infiltrate into the 3 nm pores.…”

Section: Resultsmentioning

confidence: 89%

“…[9] In our study, differential scanning calorimetry (DSC) was used to confirm the bulk phase transition temperature of all lipid structures within pores and on the outside of particles to be between 41 and 43 °C ( Figure S5, Supporting Information), which agrees with the phase transition temperature of pure DPPC bilayer vesicles. [43] The simulations predict that isolated micelles can form in smaller pores and at smaller concentrations. [41] Additionally, the fluorescent hydrocarbon diphenylhexatriene, nearly nonfluorescent in water but exhibiting extreme fluorescence in nonpolar bilayer cores, was used to confirm bilayer formation on particle surfaces using optical fluorescence microscopy (results not shown).…”

Section: Resultsmentioning

confidence: 97%

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Effects of Pore Size and Tethering on the Diffusivity of Lipids Confined in Mesoporous Silica

Schlipf

Zhou

Khan

et al. 2017

Adv Materials Inter

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Incorporation of lipid assemblies on the surface and within pores of mesoporous silica particles provides for biomimetic approaches to analyte sensing and separations using high surface area platforms. This work investigates the effect of pore confinement on the location and the diffusivity of lipid assemblies in mesoporous silica spherical particles (SBAS) as a function of nanopore diameters (nonporous, 3.0, 5.4, and 9.1 nm), which span the range of the thickness of the 1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphocholine lipid bilayer (≈4 nm). Large‐diameter SBAS are imaged with sufficient spatial resolution to distinguish lipids at the exterior surface and in the center of the particles. Lipids incorporated on the silica by evaporation deposition exist as exterior lipid bilayers on all particles and lipid assemblies in the pores of 5.4 and 9.1 nm pore diameter materials. Lipid diffusivity increases with pore size and decreases in the presence of bilayer tethering functional groups. Lipid diffusivity in the core of the particles is similar to the surface diffusivity, consistent with long‐range mobility in accessible, ordered (but randomly oriented) mesopores of SBAS materials. This work presents a framework for interpreting high density loading of lipid bilayers and their function within mesoporous materials.

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Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 97%

Effects of Pore Size and Tethering on the Diffusivity of Lipids Confined in Mesoporous Silica

Schlipf

Zhou

Khan

et al. 2017

Adv Materials Inter

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“…They also reported that the lipid diffusivity remained consistent regardless of lipid location (at the core, midway between the core and the surface, or surface) in the particle, suggesting the uniform distribution of lipid assemblies inside the pores. Similarly, dissipative particle dynamics simulations of DMPC confined in hydrophilic pores revealed that the morphology of pore-confined lipids is dependent on the pore radius ( R ) relative to the lipid length ( L ) and lipid concentration in the pores . As the lipid concentration increases, the lipid first forms micelles of distinct morphologies and then cylindrical bilayers (tubes). , The larger the ratio of pore radius to lipid length, the lower the concentration required to form cylindrical bilayers .…”

Section: Resultsmentioning

confidence: 99%

Lipid Pore-Filled Silica Thin-Film Membranes for Biomimetic Recovery of Dilute Carbohydrates

et al. 2017

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Selectively permeable biological membranes containing lipophilic barriers inspire the design of biomimetic carrier-mediated membranes for aqueous solute separation. The recovery of glucose, which can reversibly bind to boronic acid (BA) carriers, is examined in lipid pore-filled silica thin-film composite membranes with accessible mesopores. The successful incorporation of lipids (1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DPPC) and BA carriers (4-((N-Boc-amino)methyl)phenylboronic acid, BAMP-BA) in the pores of mesoporous silica (∼10 nm pore diameter) through evaporation deposition is verified by confocal microscopy and differential scanning calorimetry. In the absence of BA carriers, lipids confined inside the pores of silica thin films (∼200 nm thick) provide a factor of 14 increase in diffusive transport resistance to glucose, relative to traditional supported lipid bilayers formed by vesicle fusion on the porous surface. The addition of lipid-immobilized BAMP-BA (59 mol % in DPPC) facilitates the transport of glucose through the membrane; glucose flux increases from 45 × 10 to 225 × 10 mol/m/s in the presence of BAMP-BA. Furthermore, the transport can be improved by environmental factors including pH gradient (to control the binding and release of glucose) and temperature (to adjust lipid bilayer fluidity). The successful development of biomimetic nanocomposite membranes demonstrated here is an important step toward the efficient dilute aqueous solute upgrading or separations, such as the processing of carbohydrates from lignocellulose hydrolysates, using engineered carrier/catalyst/support systems.

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“…Dissipative Particle Dynamics (DPD) is a coarsegrained molecular simulation method to take into account hydrodynamic interactions [18][19][20]. The DPD method is applied to complex fluids such as amphiphilic molecules [15,[21][22][23][24][25][26][27][28][29][30] and polymers [20,[31][32][33]. In this method, several atoms are coarse-grained into one DPD particle, so long-term simulation can be performed compared to atomic-scale molecular dynamics.…”

Section: A Dissipative Particle Dynamicsmentioning

confidence: 99%

Morphological changes of amphiphilic molecular assemblies induced by chemical reactions

Nakagawa

Noguchi

2015

Soft Matter

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Shape transformations of amphiphilic molecular assemblies induced by chemical reactions are studied using coarse-grained molecular simulations. A binding reaction between hydrophilic and hydrophobic molecules is considered. It is found that the reaction induces transformation of an oil droplet to a tubular vesicle via bicelles and vesicles with discoidal arms. The discoidal arms close into vesicles, which are subsequently fused into the tubular vesicle. Under the chemical reaction, the bicelle-to-vesicle transition occurs at smaller sizes than in the absence of the hydrophobic molecules. It is revealed that the enhancement of this transition is due to embedded hydrophobic particles that reduce the membrane bending rigidity.

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Phase diagrams of confined solutions of dimyristoylphosphatidylcholine (DMPC) lipid and cholesterol in nanotubes

Cited by 11 publications

References 45 publications

Effects of Pore Size and Tethering on the Diffusivity of Lipids Confined in Mesoporous Silica

Effects of Pore Size and Tethering on the Diffusivity of Lipids Confined in Mesoporous Silica

Lipid Pore-Filled Silica Thin-Film Membranes for Biomimetic Recovery of Dilute Carbohydrates

Morphological changes of amphiphilic molecular assemblies induced by chemical reactions

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