Phase Mask-Based Multimodal Superresolution Microscopy

Abstract

We demonstrate a multimodal superresolution microscopy technique based on a phase masked excitation beam in combination with spatially filtered detection. The theoretical foundation for calculating the focus from a non-paraxial beam with an arbitrary azimuthally symmetric phase mask is presented for linear and two-photon excitation processes as well as the theoretical resolution limitations. Experimentally this technique is demonstrated using two-photon luminescence from 80 nm gold particle as well as two-photon fluorescence lifetime imaging of fluorescent polystyrene beads. Finally to illustrate the versatility of this technique we acquire two-photon fluorescence lifetime, two-photon luminescence, and second harmonic images of a mixture of fluorescent molecules and 80 nm gold particles with > 120 nm resolution (λ/7). Since this approach exclusively relies on engineering the excitation and collection volumes, it is suitable for a wide range of scanning-based microscopies.

Fluorescently Labeled Cellulose Nanofibers for Environmental Health and Safety Studies

“…Microscopy images of CNF. The fluorescence lifetime imaging microscopy (FLIM) of CNF samples were determined using a time-resolved single photon counting module (SPCM) set up [ 26 ]. CNF samples were drop-cast onto ultraviolet ozone (UVO)-treated glass slides, dried at room temperature, and imaged using two-photon fluorescence.…”

Development and Testing of Zinc Oxide Embedded Sulfonated Poly (Vinyl Alcohol) Nanocomposite Membranes for Fuel Cells

“…The films were nonconductive in nature; hence, gold sputtering (thickness of 15 nm) was performed on the samples. Figure 5(A) shows the pristine PVA membrane, it is observed uniform and Figure 5(B) depicts a hybrid membrane of PVA-ZnO nanoparticles, and it was discovered that the ZnO nanoparticles were dispersed across the membrane segments [33,34].…”