Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study

Brazhe, Alexey; Brazhe, Nadezda A.; Максимов, Г. В.; Ignatyev, Pavel S.; Rubin, Andrey B.; Mosekilde, Erik; Sosnovtseva, Olga

doi:10.1117/1.2937213

Cited by 20 publications

(14 citation statements)

References 27 publications

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“…4). This shape is related firstly to the cell discoid shape and secondly to uniform distribution of hemoglobin and therefore the refractive index [22]. Value of the average phase height is 191 ± 24.5 nm.…”

Section: Resultsmentioning

confidence: 99%

“…Another possible explanation is that a gradual transition of discocyte into echinocyte occurs [20,22].…”

Section: Discussionmentioning

confidence: 98%

“…was evaluated by recording the average phase height (average OPD-optical path difference), surface area and density [16][17][18][19][20][21][22][23][24][25][26].…”

Section: Methodsmentioning

confidence: 99%

“…The phase height of erythrocytes with an equal refractive index throughout the cell volume is proportional to the cell geometric height (thickness) [24,25]. From the erythrocytes phase height and refractive index values, the erythrocytes physical height was additionally calculated [22,26].…”

Section: Methodsmentioning

confidence: 99%

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Study of correlation between state and composition of lipid phase and change in erythrocytes structure under induction of oxidative processes

et al. 2015

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We investigated human blood erythrocytes under oxidative stress in vitro and established a correlation between composition and state of lipids and changes in erythrocytes structure under induced oxidative stress. These changes may serve as an indicator of not only the erythrocyte state but of systemic processes that occur at the level of the whole organism, including various pathologies as well. We found that a pyrimidine derivative xymedon used in the present study is an effective inhibitor of oxidative processes. Xymedon may be useful as an antioxidant for preserving the structural and functional characteristics of the erythrocytes in the treatment of organisms exposed to physical and toxic factors causing oxidative stress.

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Section: Resultsmentioning

confidence: 99%

“…Another possible explanation is that a gradual transition of discocyte into echinocyte occurs [20,22].…”

Section: Discussionmentioning

confidence: 98%

“…was evaluated by recording the average phase height (average OPD-optical path difference), surface area and density [16][17][18][19][20][21][22][23][24][25][26].…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Study of correlation between state and composition of lipid phase and change in erythrocytes structure under induction of oxidative processes

et al. 2015

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“…In the experiment, only samples with a 100% passage. After that, grinding efficiency also was determined sequentially using a number of techniques: laser interference microscopy (LIM) [16] with a MII-4 M at a wavelength of 473 nm and laser diffraction analysis using a SALD-3101 (Shimadzu, Japan) [17]. The biochemical composition of the raw materials was determined according to the procedure described by Yanagisawa et al [18].…”

Section: Analysis Methodsmentioning

confidence: 99%

Enzymatic hydrolysis and fermentation of ultradispersed wood particles after ultrasonic pretreatment

Ревин

Atykyan

Zakharkin

2016

Electronic Journal of Biotechnology

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a b s t r a c tBackground: A study of the correlation between the particle size of lignocellulosic substrates and ultrasound pretreatment on the efficiency of further enzymatic hydrolysis and fermentation to ethanol. Results: The maximum concentrations of glucose and, to a lesser extent, di-and trisaccharides were obtained in a series of experiments with 48-h enzymatic hydrolysis of pine raw materials ground at 380-400 rpm for 30 min. The highest glucose yield was observed at the end of the hydrolysis with a cellulase dosage of 10 mg of protein (204 ± 21 units CMCase per g of sawdust). The greatest enzymatic hydrolysis efficiency was observed in a sample that combined two-stage grinding at 400 rpm with ultrasonic treatment for 5-10 min at a power of 10 W per kg of sawdust. The glucose yield in this case (35.5 g glucose l −1 ) increased twofold compared to ground substrate without further preparation. Conclusions: Using a mechanical two-stage grinding of lignocellulosic raw materials with ultrasonication increases the efficiency of subsequent enzymatic hydrolysis and fermentation.

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Methods of Study of Neuron Structural Heterogeneity: Flow Cytometry vs. Laser Interferometry

Kopeikina

Dukhinova

Ponomarev

2018

Cellular Heterogeneity

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Neuronal cells are probably the less studied cells regarding their heterogeneity on a single cell or population levels. One of the main problems of studying of individual neurons is the presence of long processes (axons) on differentiated adult neurons that hamper their isolation without significant damage to the cells. Therefore, the most common method to study neuronal cells is immunofluorescent microscopy of sections of the brain, which remains poorly quantitative and allows analyzing a small number of fixed cells. Also, immunofluorescent microscopy has a number of staining artifacts since histology section has high level of autofluorescence and non-specific binding of fluorescent probes. Alternative methods that could overcome disadvantages of immunofluorescent histology include flow cytometry, scanning cytometry, and laser interferometry. Flow cytometry and, to some extent of degree, scanning cytometry allow performing analysis of multiple markers with a low level of non-specific background and very robust statistics. Laser interferometry allows studies intact, alive neurons without staining. Limitations and advantages of these methods are discussed in this chapter.

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Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study

Cited by 20 publications

References 27 publications

Study of correlation between state and composition of lipid phase and change in erythrocytes structure under induction of oxidative processes

Study of correlation between state and composition of lipid phase and change in erythrocytes structure under induction of oxidative processes

Enzymatic hydrolysis and fermentation of ultradispersed wood particles after ultrasonic pretreatment

Methods of Study of Neuron Structural Heterogeneity: Flow Cytometry vs. Laser Interferometry

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