Phase Partitioning in Space and on Earth

Alstine, James M. Van; Karr, Laurel J.; Harris, J. Milton; Snyder, Robert S.; Bamberger, Stephan; Matsos, Helen C.; Curreri, Peter A.; Boyce, J. F.; Brooks, Donald E.

doi:10.1007/978-1-4684-5442-0_27

Cited by 9 publications

(7 citation statements)

References 49 publications

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“…The regional expression pattern of mouse CRISP-1 (and of rat DE/AEG) in the epididymis parallels the appearance of motility and of fertilizing potential of spermatozoa (Van Alstine et al, 1987). Based on these observations, it has been proposed that this protein plays a direct role in sperm maturation, possibly in the acquisition of motility.…”

Section: Discussionmentioning

confidence: 93%

Mouse androgen‐dependent epididymal glycoprotein crisp‐1 (DE/AEG): Isolation, biochemical characterization, and expression in recombinant form

Eberspaecher¹,

Roosterman²,

Krätzschmar³

et al. 1995

Molecular Reproduction Devel

100

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In the rat, the secretory glycoprotein DE/AEG is one of the main constituents of the epididymal fluid. We have recently reported the cloning of the cDNA for the related cysteine-rich secretory protein-1 (CRISP-1) from murine epididymis (Haendler et al., 1993; Endocrinology 133:192-198). The protein has now been isolated from the same organ and its N-terminal amino acid sequence has been determined. CRISP-1 exhibited an isoelectric point of approximately 6.8. High levels of CRISP-1 antigen were detected in the corpus and cauda of the epididymis, vas deferens, seminal vesicle, prostate, and in the salivary gland by immunohistochemistry. A quantitative analysis of the cauda epididymal fluid by sandwich ELISA revealed that CRISP-1 represented approximately 15% of the total protein. For heterologous expression, the CRISP-1 coding sequence was introduced into the pMPSV/CMV vector before transfection of baby hamster kidney (BHK) cells and selection with puromycin and neomycin. Expression in insect cells was achieved by co-transfection of Sf9 cells with a transfer vector and baculovirus DNA. Recombinant CRISP-1 was isolated in quantities sufficient for structural analysis. Ethyl maleimide treatment showed that all 16 cysteines were engaged in disulfide bonds. Proteolytic digestion demonstrated that the six cysteines localized in the N-terminal moiety formed three bonds with each other, suggesting the existence of two discrete domains in the protein.

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Section: Discussionmentioning

confidence: 93%

Mouse androgen‐dependent epididymal glycoprotein crisp‐1 (DE/AEG): Isolation, biochemical characterization, and expression in recombinant form

Eberspaecher¹,

Roosterman²,

Krätzschmar³

et al. 1995

Molecular Reproduction Devel

100

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“…As a consequence of the latter, these droplets move in the continuous phase in the presence of an electric field [18][19][20].…”

Section: Theoretical Aspectsmentioning

confidence: 99%

Electrokinetic demixing of aqueous two-phase polymer/salt systems

N¹,

Chethana²,

Raghavarao³

2005

Electrophoresis

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Electrokinetic demixing of aqueous two-phase polymer/salt systems is demonstrated, resulting in significant enhancement in demixing rates by about 1-4-fold. The effect of field polarity, field strength, volume ratio, and phase composition on phase demixing has been studied. Further the influence of these parameters on phase demixing could be explained based on the hydrodynamic flow-electroosmotic flow (HEF) model.

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“…149 ' 156 -165 ' 166 In hydrophilic vessels dextran forms a "shell" around the PEG phase, and in hydrophobic vessels PEG forms a shell around the dextran phase. 151 Thus, surface forces, presumably interfacial tension and gradients thereof and wall wetting, can drive phase separation in the absence of buoyancy. This supposition was confirmed in laboratory experiments in which added polymers were used to make the densities of the two phases as nearly equal as possible 151 and in which vessel surfaces were deliberately coated with hydrophilic polymers.…”

Section: Phase Separation In Biphasic Aqueous Systems In Low Gravitymentioning

confidence: 99%

“…151 Thus, surface forces, presumably interfacial tension and gradients thereof and wall wetting, can drive phase separation in the absence of buoyancy. This supposition was confirmed in laboratory experiments in which added polymers were used to make the densities of the two phases as nearly equal as possible 151 and in which vessel surfaces were deliberately coated with hydrophilic polymers. 167 Modifying the relative volumes of the two phases affected the rate of phase demixing in low gravity.…”

Section: Phase Separation In Biphasic Aqueous Systems In Low Gravitymentioning

confidence: 99%

“…These findings were the result of a search for a method for driving phase separation in low-gravity experiments. 151 It should be possible to control dimixing rates and possibly enhance demixing of phases with small density differences by the application of an electric field to two-phase polymer emulsions in practical applications. 152 To test this hypothesis, a vertical electrophoresis column of the type shown in Fig.…”

Section: Phase Separationmentioning

confidence: 99%

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