Phase retrieval of microscope objects using the Wavelet-Gabor transform method from holographic filters

Abstract

An analysis of an optical-digital system based on the architecture of the Mach-Zehnder interferometer for recording holographic filters is presented. The holographic recording system makes use of one microscope objective in each interferometer arm. Moreover, the Gabor Wavelet Transform is implemented for the holographic reconstruction stage. The samples studied of this research are selected in order to test the retrieval algorithm and to characterize the resolution of the holographic recording system. In this last step, some sections of an USAF1951 resolution chart are used. These samples allow us to study the features of lighting in the recorded system. Additionally, some organic samples are used to proven the capabilities of the method because biological samples have much complex morphological composition than others. With this in mind, we can verify the frequencies recovered with each of the settings set in the retrieval method. Experimental results are presented.