Phase separations in membranes of Anacystis nidulans grown at different temperatures

Furtado, D.; Williams, W.P.; Brain, Anthony P.R.; Quinn, Peter J.

doi:10.1016/0005-2736(79)90174-3

Cited by 53 publications

(8 citation statements)

References 11 publications

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“…1, A and B) particle-free regions were seen in almost all of the PF of the cytoplasmic membrane. This is in agreement with previous reports by other investigators (1,2,4,16). At 13°C, the particle-free regions were small (Fig.…”

supporting

confidence: 94%

“…In the temperature range between 13 and 19°C, the cytoplasmic membrane undergoes the phase change from the liquid-crystalline to the phase-separation state. A net-like distribution of particles, as reported by Furtado et al (4), was not found at any temperature of fixation.…”

supporting

confidence: 61%

“…Midpoint values for critical temperatures of the inactivation of photosynthesis were 4 and 12°C in cells grown at 28 and 38°C, respectively (11). Potassium ions and free amino acids leaked to the outer medium below these temperatures (12).…”

mentioning

confidence: 99%

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Chilling-Susceptibility of the Blue-Green Alga Anacystis nidulans

Ono¹,

Murata²

1982

Plant Physiol.

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The lipid phase of cytoplasmic membrane was studied by freeze-fracture electron microscopy in the chilling-susceptible blue-green alga, Anacystis nidulans. At growth temperatures, intramembrane particles were distributed at random in the fracture faces of cytoplasmic membrane, whereas, at chilling temperatures, the fracture faces were composed of particle-free and particle-containing regions. These findings indicate that lipids of the cytoplasmic membrane were in the liquid-crystalline state at the growth temperatures and in the phase-separation state at the chilling temperatures.

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confidence: 94%

supporting

confidence: 61%

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Chilling-Susceptibility of the Blue-Green Alga Anacystis nidulans

Ono¹,

Murata²

1982

Plant Physiol.

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“…This may be inferred from results obtained on microbial systems (Sinenski, 1974;Martin et al, 1981;Janoff et al, 1979;Dickens and Thompson, 1981), plants (Chapman et al, 1979;Furtado et al, 1979;Vigh et al, 1979Vigh et al, , 1986, Tetrahymena (Umeki et al, 1983), and some poikilothermic animals (Kallapur et al, 1982;Cossins, 1977;Farkas, 1984). Thus we may speculate that the seasonal occurrence or geographical distribution of different species is related to their ability to manipulate cell membranes in order to survive at a given temperature .…”

Section: Introductionmentioning

confidence: 87%

The fatty acid constitution and ordering state of membranes in dominant temperature-sensitive lethal mutation and wild-typeDrosophila melanogaster larvae

1990

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The ordering state and changes in fatty acid composition of microsomal (MS) and mitochondrial (MC) membranes of two dominant temperature-sensitive (DTS) lethal mutations and the wild-type Oregon-R strain larvae of Drosophila melanogaster have been studied at 18 and 29 degrees C and after temperature-shift experiments. The membranes of wild-type larvae have a stable ordering state, with "S" values between 0.6 (18 degrees C) and 0.5 (29 degrees C) in both membranes which remained unchanged in shift experiments, although the ratios of saturated/unsaturated fatty acids were changed as expected. The strongly DTS mutation 1(2) 10DTS forms very rigid membranes at the restrictive temperature (29 degrees C) which cannot be normalized after shift down, while shift up or development at the permissive temperature results in normal ordering state. This mutant is less able to adjust MS and MC fatty acid composition in response to the growth temperature than the wild type. The less temperature-sensitive 1(2)2DTS allele occupies an intermediate state between Oregon-R and 1(2)10DTS in both respects. We assume and the genetical data suggest that the DTS mutant gene product is in competition with the wild-type product, resulting in a membrane structure which is not able to accommodate to the restrictive temperature.

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“…Changes in lipid phase with temperature have been detected within the membranes of Anacystis using spin probe (10), x-ray diffraction (22) methods, or freeze-fracture EM (5). These physical techniques however, yield conflicting results.…”

mentioning

confidence: 99%

Primary Role of the Cytoplasmic Membrane in Thermal Acclimation Evidenced in Nitrate-Starved Cells of the Blue-Green Alga, Anacystis nidulans

Gombos

Vı́gh²

1986

Plant Physiol.

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The lipid phase transition of the cytoplasmic membrane and the chilling susceptibility were studied in nitrate-starved Anacystis nidulans cells. Nitrate starvation resulted in the disappearance of the thylakoid membrane system, without any effect on chilling susceptibility. The chilling susceptibility of the algal cells depended on the growth temperature. Temperatures of lipid phase transitions of the cytoplasmic membranes were detected by chilling-induced spectral changes in the carotenoid region, in vivo. These values were identical to those of cultures containing intact thylakoid systems. Our results suggest that cytoplasmic membrane plays a determinative role in the thermal acclimation of the alga cells.The blue-green alga, Anacystis nidulans, offers an effective experimental system for the study of the relationship between growth temperature, cold sensitivity, and thermotropic changes in membranes. Although this alga is a prokaryotic organism, it can be regarded as a model system for the study of the temperature effects on higher plants ( 18). As reported by Murata et al. (9,10), photosynthetic evolution, Hill activity to benzoquinone, and the bleaching of membrane pigments in Anacystis cells show identical temperature dependences. The optimum temperatures associated with these phenomena are a function of the growth temperature of the cultures (13). These optimum temperatures, however, were found to be considerably lower than the phase transitions detected in the thylakoid membranes, but they were in very good agreement with those for release of potassium ions and ninhydrin-reacting substances (14). These data suggest that it is a phase separation occurring in the cytoplasmic membrane which leads to an enhanced permeability of the cells.Changes in lipid phase with temperature have been detected within the membranes of Anacystis using spin probe (10), x-ray diffraction (22) methods, or freeze-fracture EM (5). These physical techniques however, yield conflicting results. Even using freeze-fracture technique, which allows a direct visualization of the segregation of smooth regions of gel-phase lipids within fractured faces, the onset of phase separation of the cytoplasmic membrane is found to be considerably higher in a study of Armond and Staehelin (2) than observed by Ono and Murata (15) using Anacystis nidulans cells grown at identical temperatures (38°C).To prove the validity of the mechanism attributing primary role ofcytoplasmic membrane in thermal adaptation and chilling susceptibility, we used nitrate-starved Anacystis nidulans cell system. As described elsewhere (6), growing the algal cells in nitrogen-free culturing medium results in a rapid degradation, or even a complete breakdown, of the photosynthetic apparatus. During this process, the lipids of the alga cells are catabolized, and concurrent changes in the composition of carotenoids can also be detected (6,17). By adapting the method of nitrate starvation, we were able to obtain Anacystis cells, grown at various temperatures, which had ...

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Phase separations in membranes of Anacystis nidulans grown at different temperatures

Cited by 53 publications

References 11 publications

Chilling-Susceptibility of the Blue-Green Alga Anacystis nidulans

Chilling-Susceptibility of the Blue-Green Alga Anacystis nidulans

The fatty acid constitution and ordering state of membranes in dominant temperature-sensitive lethal mutation and wild-typeDrosophila melanogaster larvae

Primary Role of the Cytoplasmic Membrane in Thermal Acclimation Evidenced in Nitrate-Starved Cells of the Blue-Green Alga, Anacystis nidulans

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