Phase transition characteristics of diphosphatidylglycerol (cardiolipin) and stereoisomeric phosphatidyldiacylglycerol bilayers

Rainier, Shirley; Jain, Mahendra Kumar; Ramírez, Fausto; Ioannou, Panayiotis V.; Marecek, James F.; Wagner, Richard

doi:10.1016/0005-2736(79)90059-2

Cited by 59 publications

(22 citation statements)

References 25 publications

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“…This transition in shape could be explained by crossing the temperature of the main gel-to-liquid crystal-phase transition. Our observations correspond to the previous results 6,7 where the temperature of Na salt of cardiolipin was determined to be 39.7°C…”

Section: Discussionsupporting

confidence: 92%

Shape and Size of Giant Unilamellar Phospholipid Vesicles Containing Cardiolipin

Tomšiè

Babnik

Lombardo

et al. 2005

J. Chem. Inf. Model.

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The effect of cardiolipin content on the shape and size of giant palmitoyloleylphosphatidylcholine/cardiolipin vesicles was studied. Unilamellar vesicles were prepared in sugar solution by the method of electroformation, from mixtures containing up to 50% weight ratio of cardiolipin. At room temperature the vesicles containing cardiolipin exhibited abrupt changes in the curvature of the vesicle contour indicating regions of phase separation. The deviations from the spherical shape were larger if vesicles were made from mixtures with a higher content of cardiolipin. Numerous vesicles with soft fluctuating walls were observed. The estimated size of the vesicles containing cardiolipin was found to be smaller than the size of pure palmitoyloleylphosphatidylcholine vesicles.

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Section: Discussionsupporting

confidence: 92%

Shape and Size of Giant Unilamellar Phospholipid Vesicles Containing Cardiolipin

Tomšiè

Babnik

Lombardo

et al. 2005

J. Chem. Inf. Model.

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“…ted as phosphatidyldiacylglycerol or 4R-bis-PA, R = acyl chain, bis-PA ='bis-phosphatidic acid', were sythesized as previously described [8,9]. The purity of the phospholipid samples was established by thin-layer chromatography [6][7][8][9] and elemental analysis.…”

Section: Sn-glycerol-3-o-phosphoryl)-sn-glycerol Abbrevia-mentioning

confidence: 99%

“…The purity of the phospholipid samples was established by thin-layer chromatography [6][7][8][9] and elemental analysis. The 1,2-diacyl-sn-glycerols employed in the synthesis of phosphatidylcholesterol and phosphatidyldiacylglycerol were prepared from freshly distilled acyl chlorides shown to be 99.9% and 99.0% pure, respectively, by gas chromatographic analysis of the corresponding methyl esters [7].…”

Section: Sn-glycerol-3-o-phosphoryl)-sn-glycerol Abbrevia-mentioning

confidence: 99%

Bilayers of phosphatidyldiacylglycerol and phosphatidylcholesterol give 31P-NMR spectra characteristic for hexagonal and isotropic phases

Noggle

Marecek

Mandal

et al. 1982

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Aqueous dispersions of phosphatidyidiacylglyceroi and phosphatidyicholesteroi are shown to form bilayers by differential scanning calorimetry, diphenylhexatriene fluorescence polarization, and electron microscopy; however, 3~ P-nuclear magnetic resonance spectra of these dispersions are characteristic of the 'hexagonal' and 'isotropic' phases, respectively. The theoretical relationship between the conformation of phospholipid molecules in bilayers and the shape of the 31 P-NMR line is examined. It is shown that differences in the various characteristic spectral line shapes can result from differences in the phospholipid headgroup conformation without significant changes in the organization of the acyi chains in the bilayer phase.

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“…3, the increase in unsaturation in CL acyl chains from hormonally- CL is known for its tendency to adopt an organization in structures with concave waterlipid interfaces in response to the presence of divalent cations such as Ca 2+. However, the bilayer-nonbilayer transition has been shown to be dependent on the fatty acid composition of CL since the bacterial CL, which has a more saturated fatty acid composition than the bovine heart CL, prefers a bilayer organization (21). Since hCG has been shown to increase cytosolic free calcium in rat Leydig cells (22) and as a higher degree of unsaturation leads to a greater tendency to form aggregate structures with concave interfaces such as the non-bilayer inverted hexagonal phase (23,24), it is apparent from our results that an increase in CL unsaturation on hCG treatment might favor the non-bilayer arrangement.…”

Section: Resultsmentioning

confidence: 99%

Hormone‐induced changes in cardiolipin from leydog cells: Possible involvement in intramitochondrial cholesterol translocation

et al. 1998

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SUMMARY : The rate-limiting and hormonally regulated step in steroid hormone biosynthesis is the delivery of cholesterol from the outer to the inner mitochondrial membrane where cytochrome P450scc resides. Although the exact mechanism of intramitochondrial cholesterol translocation remains unknown, the formation of contact sites between outer and inner mitochondrial membranes appears as a necessary component for cholesterol transfer. Several pieces of evidence suggest that local formation of intermembrane contact is a consequence of a non-bilayer arrangement of polymorphic lipids which are enriched in the junctions. As a step toward clarifying mitochondrial contact sites formation and thus cholesterol translocation in steroidogenic cells, we have undertaken studies to identify the factors which might result in non-bilayer structure to be adopted by mitochondrial phospholipids on stimulation of MA-10 Leydig cells. Our results demonstrate that an increase in the unsaturation of the cardiolipin acyl groups on hormonal stimulation might favor the formation of non-bilayer adhesion points.

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Phase transition characteristics of diphosphatidylglycerol (cardiolipin) and stereoisomeric phosphatidyldiacylglycerol bilayers

Cited by 59 publications

References 25 publications

Shape and Size of Giant Unilamellar Phospholipid Vesicles Containing Cardiolipin

Shape and Size of Giant Unilamellar Phospholipid Vesicles Containing Cardiolipin

Bilayers of phosphatidyldiacylglycerol and phosphatidylcholesterol give 31P-NMR spectra characteristic for hexagonal and isotropic phases

Hormone‐induced changes in cardiolipin from leydog cells: Possible involvement in intramitochondrial cholesterol translocation

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