Phaseollidin stored in vacuoles and the phytoalexin response in bean

Soriano‐Richards, E.; Uribe-Salas, Dolores; Ibarra‐Barrera, G.

doi:10.1046/j.1365-3059.1998.00264.x

Cited by 7 publications

(5 citation statements)

References 30 publications

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“…The antibacterial activity of the tissue extracts correlated with their levels of phaseollin. Previous studies reported that all of the phytoalexins isolated from bean had antibacterial and antifungal properties (Gnanamanickam and Patil 1977a;Bailey et al 1980;Soriano-Richards et al 1998). Close association between plant cell death and the level of phaseollin accumulation was also revealed in this study.…”

Section: Discussionsupporting

confidence: 79%

Determination of responses of different bean cultivars against races of Pseudomonas syringae pv phaseolicola, causal agent of halo blight of bean

Bozkurt

Soylu

2011

Euphytica

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Use of resistant plant varieties combined with other disease management practices is regarded as the most practical approach to control of seedborne bacterial disease agents. In this study, responses of different bean cultivars to nine different races of Pseudomonas syringae pv phaseolicola, the causal agent of bacterial halo blight of common bean (Phaseolus vulgaris L.), were determined. During compatible interaction in susceptible cultivars, virulent bacterial races caused water soaked lesion at sites of inoculation. Similar lesions developed in moderately resistant cultivars but symptoms were later associated with more tissue browning around the sites of inoculation. In contrast, the resistant response, produced the characteristic hypersensitive reaction (HR), was characterized as a small discrete browning and tissue collapse at site of inoculation. No local cultivars showed complete resistance to all races tested. Bean cultivars Sehirali-90 and Göynük-98 were found to be resistant or moderately resistant to five different bacterial races. Bean cultivar, Karacaşehir-90, on the other hand, was found to be resistant or moderately resistant to six different bacterial races. Analysis of bacterial growth and the accumulation of isoflavonoid bean phytoalexin, phaseollin in planta were carried out for tissues expressing compatible and incompatible interactions to enable a link to be made between reaction phenotypes and restriction of bacterial growth and phytoalexin accumulations. Development of the HR was clearly associated with the restricted multiplication of bacteria during incompatible interactions. A time-course accumulation analysis on pods treated with different races of bacterial agent showed that a strong correlation was observed between the timing and extent of cell death and accumulation of phaseollin, being rapid and extensive in incompatible interactions compared to compatible interaction.

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Section: Discussionsupporting

confidence: 79%

Determination of responses of different bean cultivars against races of Pseudomonas syringae pv phaseolicola, causal agent of halo blight of bean

Bozkurt

Soylu

2011

Euphytica

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“…1996; Hutzler et al . 1998; Soriano‐Richards, Uribe‐Salas & Ibarra‐Barrera 1998; Markham et al . 2000).…”

Section: Discussionunclassified

Section: Discussionmentioning

confidence: 99%

“…On the other hand, intracellular localization of flavonoids remains rather elusive, and various compartments have been indicated (Schnitzler et al 1996;Hutzler et al 1998;Soriano-Richards, Uribe-Salas & Ibarra-Barrera 1998;Markham et al 2000). It seems that the major difficulty is related to technical problems.…”

Section: Discussionmentioning

confidence: 99%

“…It seems that the major difficulty is related to technical problems. Various approaches were used to determine subcellular location of phenolic compounds, including isolation of vacuoles (Soriano-Richards et al 1998), confocal laser scanning microscopy (Hutzler et al 1998), and isolation of protoplasts followed by cell wall digestion (Markham et al 2000). Although in the latter two cases flavonoids have been located in cell walls, respective β-glucosidases were not colocalized, and both approaches presented some important limitations.…”

Section: Discussionmentioning

confidence: 99%

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Cell wall‐associated isoflavonoids andβ‐glucosidase activity inLupinus albusplants responding to environmental stimuli

Piślewska

Bednarek

Stobiecki

et al. 2002

Plant Cell & Environment

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Plants respond to various biotic or abiotic stimuli with different mechanisms and the mobilization of phenolic compounds is one of them. In white lupin ( Lupinus albus L.) plants, isoflavones and their glucosides were localized in cell walls where the high constitutive activity of β β β β -glucosidase (EC 3·2·1·21) was also identified. The enzyme was partially purified from root cell walls. Its polymeric active form has 180 or 200 kDa as determined by non-denaturing electrophoresis and gel filtration, respectively, and the isoelectric point is at pH 6·9. The enzyme is an exoglucosidase, preferentially hydrolysing conjugates of phenolic compounds with β β β β anomers of glucose. It is not active against purified lupin cell walls. The specific β β β β -glucosidase activity varies in different tissues with the highest one in roots, and always higher in cell walls than in protoplast. The cell wall location of the enzyme was confirmed biochemically by its activity in intercellular washing fluids. Both aglycones and glycosides were also present in these fluids. The specific β β β β -glucosidase activity correlated well with the isoflavonoid aglycone/glycoside ratios in various tissues: the higher β β β β -glucosidase activity, the higher relative aglycone content. β β β β -glucosidase activity was found to be inducible under conditions of yeast elicitor treatment. Induction of the enzyme was accompanied by changes in the isoflavone secretion and accumulation, suggesting the regulatory role of β β β β -glucosidase activity in root exudation of isoflavonoids. No correlation however, was found between changes in β β β β -glucosidase activity and the presence of isoflavonoids in root exudates of plants grown under various nitrogen nutrition regimes.

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The Chemistry of The Genus Cicer L.

Stevenson

Aslam

2006

Studies in Natural Products Chemistry

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Phaseollidin stored in vacuoles and the phytoalexin response in bean

Cited by 7 publications

References 30 publications

Determination of responses of different bean cultivars against races of Pseudomonas syringae pv phaseolicola, causal agent of halo blight of bean

Determination of responses of different bean cultivars against races of Pseudomonas syringae pv phaseolicola, causal agent of halo blight of bean

Cell wall‐associated isoflavonoids andβ‐glucosidase activity inLupinus albusplants responding to environmental stimuli

The Chemistry of The Genus Cicer L.

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