Phaseolus vulgaris cultivar and Rhizobium strain variation in acid-pH tolerance and nodulation under acid conditions

Vargas, A A T; Graham, Peter

doi:10.1016/0378-4290(88)90047-0

Cited by 80 publications

(34 citation statements)

References 21 publications

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“…Strains tolerant to acid soil conditions will often, but not always, nodulate better than acid-sensitive strains at low soil pH (Graham et ai., 1982;Howieson and Ewing, 1986). Thus, while R. et/i inoculant strains are generally more competitive in nodule formation for P. vulgaris than are strains of R. tropici (Martinez and Rosenbleuth, 1991;Chaverra and Graham, 1992), the latter organisms will generally dominate under acid soil conditions (Frey and Blum, 1994;Streit et al, 1992;Vargas and Graham, 1988). Similarly Buendia-Claveria et al, (1994) found S. fredii generally more competitive than B. japonicum in more neutral Spanish soils.…”

Section: Va Soil Aciditymentioning

confidence: 86%

Soil Biology of the Rhizobiaceae

Sadowsky¹,

Graham²

1998

The Rhizobiaceae

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Section: Va Soil Aciditymentioning

confidence: 86%

Soil Biology of the Rhizobiaceae

Sadowsky¹,

Graham²

1998

The Rhizobiaceae

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“…Therefore, the population of beannodulating rhizobia in soils of Arcos de Valdevez was shown to be diverse, which allowed the selection of representative isolates for further analysis. Fingerprint pattern C was also obtained with the R. tropici type IIA strain CFN299 (CENA 183), which was isolated from an acid soil in Brazil (28).…”

Section: Resultsmentioning

confidence: 99%

Evidence of an American Origin for Symbiosis-Related Genes in Rhizobium lusitanum

Valverde

Velázquez

Cervantés

et al. 2011

Appl Environ Microbiol

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Randomly amplified polymorphic DNA (RAPD) analysis was used to investigate the diversity of 179 bean isolates recovered from six field sites in the Arcos de Valdevez region of northwestern Portugal. The isolates were divided into 6 groups based on the fingerprint patterns that were obtained. Representatives for each group were selected for sequence analysis of 4 chromosomal DNA regions. Five of the groups were placed within Rhizobium lusitanum, and the other group was placed within R. tropici type IIA. Therefore, the collection of Portuguese bean isolates was shown to include the two species R. lusitanum and R. tropici. In plant tests, the strains P1-7, P1-1, P1-2, and P1-16 of R. lusitanum nodulated and formed nitrogen-fixing symbioses both with Phaseolus vulgaris and Leucaena leucocephala. A methyltransferase-encoding nodS gene identical with the R. tropici locus that confers wide host range was detected in the strain P1-7 as well as 24 others identified as R. lusitanum. A methyltransferase-encoding nodS gene also was detected in the remaining isolates of R. lusitanum, but in this case the locus was that identified with the narrow-host-range R. etli. Representatives of isolates with the nodS of R. etli formed effective nitrogen-fixing symbioses with P. vulgaris and did not nodulate L. leucocephala. From sequence data of nodS, the R. lusitanum genes for symbiosis were placed within those of either R. tropici or R. etli. These results would support the suggestion that R. lusitanum was the recipient of the genes for symbiosis with beans from both R. tropici and R. etli. Common bean (Phaseolus vulgaris)is an important agricultural leguminous crop that has the ability to nodulate with rhizobia from at least 20 different host plant genera (summarized in reference 1). Among bean-nodulating rhizobia, the host range varies from narrow, as is the case for Rhizobium etli strain CFN42 (13), to wide, as for example the Rhizobium tropici IIA and IIB strains CFN299 and CIAT899 (14). Wide host range in bean-nodulating rhizobia was defined as the ability to form a symbiosis with the tropical tree Leucaena leucocephala (14) requiring the R. tropici nodS gene downstream of nodC (32). In complementation analysis, the R. tropici nodS gene was shown to extend the host range of R. etli to include L. leucocephala (32).According to Graham and Ranalli (6), common bean was introduced as a curiosity from the Americas into Europe via the Iberian Peninsula during the 16th century (34), but today it is a widely cultivated crop in Spain (21) and Portugal (19). Descriptions of bean rhizobia from the Iberian Peninsula have been made with isolates that originated from soils of Spain (8,20) and Portugal (25). The majority of the isolates originating from Spain were characterized as R. etli, R. gallicum, and R. giardinii (8,20), probably imported from the Americas (4, 14, 23, 35) along with the seeds (16). However, the isolates of Portuguese origin were proposed to represent the new species R. lusitanum, having characters that are different from ...

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“…Isto pode acontecer porque existem respostas peculiares entre os genótipos resultantes da expressão genética, influenciando a distribuição dos elementos ou a sensibilidade de sistemas metabólicos. Além disso, para a utilização da tecnologia de inoculação, são necessárias pesquisas para determinar as melhores estirpes a serem utilizadas como inoculantes numa determinada região, pois estas sofrem interferências das condições edafo-climáticas como acidez, disponibilidade de nutrientes, temperatura e umidade do solo (Vargas & Graham, 1988;Kahindi et al, 1997, Hungria & Vargas, 2000, Andrade et al, 2002, Hara & Oliveira, 2005Moreira & Siqueira, 2006). Pelo caráter promíscuo da nodulação do feijão-caupi, espécies nativas presentes no solo podem colonizar as raízes, competindo com a cepa inoculante pelos sítios de infecção (Thies et al, 1991).…”

Section: Introductionunclassified

Produtividade do feijão-caupi cv BR 17 Gurguéia inoculado com bactérias diazotróficas simbióticas no Piauí

Almeida

Alcantara

Nóbrega

et al. 2010

Agraria

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Produtividade do feijão-caupi cv BR 17 Gurguéia inoculado com bactérias diazotróficas simbióticas no Piauí RESUMOO feijão-caupi é de grande importância para as regiões Norte e Nordeste do Brasil pela sua adaptabilidade a condições edafo-climáticas e por ser uma das principais fontes de proteína para as populações dessas regiões. Assim, neste trabalho objetivou-se selecionar cepas de bactérias diazotróficas simbióticas para serem utilizadas como inoculantes para a cultura do feijão-caupi (Vigna unguiculata (L.) Walp), na região Meio-Norte do Estado do Piauí. O experimento foi instalado na Estação Experimental da Embrapa Meio-Norte, localizada em Teresina, PI. Os tratamentos foram constituídos por quatro inoculações de cepas recomendadas pelo Ministério da Agricultura Pecuária e Abastecimento (MAPA) para a cultura do feijão-caupi INPA 03-11b (BR 3301), UFLA 03-84 (BR 3302), BR 3267 e BR 3262, e a cepa a ser também testada BR 3299, duas testemunhas com adição de adubação nitrogenada (50 kg ha -1 no plantio e 80 kg ha -1 , sendo 40 kg no plantio e de 40 kg em cobertura aos 30 dias após o plantio) e uma testemunha sem adubação nitrogenada e sem inoculação. As cepas recomendadas INPA 03-11b (BR 3301), BR 3267, BR 3262 e BR 3267 e a cepa testada BR 3299 responderam satisfatoriamente aos testes de eficiência agronômica, apresentando alto potencial para também serem recomendadas como inoculantes para o feijão-caupi na região Meio-Norte do Estado do Piauí. Palavras-chave: Bradyrhizobium, fixação biológica de N 2 , Vigna unguiculataCowpea cv BR17 Gurguéia yield inoculated with symbiotic diazotrophic bacteria in Piauí ABSTRACT Cowpea bean is an important culture for the North and Northeast regions in Brazil for its adaptability to soilclimatic changes and for being one of the main protein sources for these regions population. Therefore, this work aimed to select symbiotic diazotrophic bacteria strains to be used as inoculants for Cowpea bean (Vigna unguiculata (L.) Walp) cultivation, in the Middle North region of the Piauí State, Brazil. The experiment was installed into Middle North EMBRAPA Experimental Station, located in the municipality of Teresina, PI, Brazil. Treatments consisted of four strains inoculations recommended by the Ministry of Agriculture, Livestock and Food Supply (MAPA) for cultivation of the cowpea bean INPA 03-11b (BR 3301), UFLA 03-84 (BR 3302), BR 3267 and BR 3262, and also the strain BR 3299 to be tested, two witness with nitrogen fertilization addition (50 kg ha -1 during plantation and; 80 kg ha -1 , 40 kg during plantation and 40 kg in covering layer 30 days after plantation) and one witness without nitrogen adubation and without inoculation. Recommended strains INPA 03-11b (BR 3301), BR 3267, BR 3262 and BR 3267 and the tested strain BR 3299 responded satisfactorily to agronomic efficiency tests and presented high potential for use as inoculants for cowpea bean in the Middle North region of Piauí.

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Phaseolus vulgaris cultivar and Rhizobium strain variation in acid-pH tolerance and nodulation under acid conditions

Cited by 80 publications

References 21 publications

Soil Biology of the Rhizobiaceae

Soil Biology of the Rhizobiaceae

Evidence of an American Origin for Symbiosis-Related Genes in Rhizobium lusitanum

Produtividade do feijão-caupi cv BR 17 Gurguéia inoculado com bactérias diazotróficas simbióticas no Piauí

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