Phaseolus vulgaris is a non-selective host for nodulation

Michiels, Jan; Dombrecht, Bruno; Vermeiren, Nicolas; Xi, Chuanwu; Luyten, Ellen; Vanderleyden, Jos

doi:10.1111/j.1574-6941.1998.tb00505.x

Cited by 126 publications

(53 citation statements)

References 28 publications

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“…Apart from the four strains mentioned above, which did not nodulate any plant, all bradyrhizobia induced whitish and irregularly shaped nodule-like structures on common bean, as previously observed (49). The plants showed clear symptoms of N shortage, and no N 2 fixation could be detected by ARA (see Fig.…”

Section: Isolation Of Root Nodule Rhizobia From the Okavango Regionmentioning

confidence: 62%

Rhizobia Indigenous to the Okavango Region in Sub-Saharan Africa: Diversity, Adaptations, and Host Specificity

Grönemeyer

Kulkarni

Hölscher

et al. 2014

Appl Environ Microbiol

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The rhizobial community indigenous to the Okavango region has not yet been characterized. The isolation of indigenous rhizobia can provide a basis for the formulation of a rhizobial inoculant. Moreover, their identification and characterization contribute to the general understanding of species distribution and ecology. Isolates were obtained from nodules of local varieties of the pulses cowpea, Bambara groundnut, peanut, hyacinth bean, and common bean. Ninety-one of them were identified by BOX repetitive element PCR (BOX-PCR) and sequence analyses of the 16S-23S rRNA internally transcribed spacer (ITS) and the recA, glnII, rpoB, and nifH genes. A striking geographical distribution was observed. Bradyrhizobium pachyrhizi dominated at sampling sites in Angola which were characterized by acid soils and a semihumid climate. Isolates from the semiarid sampling sites in Namibia were more diverse, with most of them being related to Bradyrhizobium yuanmingense and Bradyrhizobium daqingense. Host plant specificity was observed only for hyacinth bean, which was nodulated by rhizobia presumably representing yet-undescribed species. Furthermore, the isolates were characterized with respect to their adaptation to high temperatures, drought, and local host plants. The adaptation experiments revealed that the Namibian isolates shared an exceptionally high temperature tolerance, but none of the isolates showed considerable adaptation to drought. Moreover, the isolates' performance on different local hosts showed variable results, with most Namibian isolates inducing better nodulation on peanut and hyacinth bean than the Angolan strains. The local predominance of distinct genotypes implies that indigenous strains may exhibit a better performance in inoculant formulations.T he catchment area of the Okavango River extends across parts of the three sub-Saharan African countries of Angola, Namibia, and Botswana and is mainly characterized by smallholder and subsistence farming. Since cultivation techniques remain underdeveloped, e.g., in terms of irrigation and the application of agrochemicals or rhizobial inoculants, local farmers are confronted with low yields and decreasing soil fertilities (1). Pulses like cowpea (Vigna unguiculata), Bambara groundnut (Vigna subterranea), peanut (Arachis hypogaea), hyacinth bean (Lablab purpureus), and common bean (Phaseolus vulgaris) are cultivated and occasionally intercropped, but natural nodulation rates are often low (2), presumably due to a seasonal reduction of the rhizobial population caused by drought and heat. Thus, nitrogen input via symbiotic N 2 fixation might be very limited.Field-grown cowpea plants in Ghana have been reported to fix more than 200 kg N/ha (89% of plant N) (3). In contrast, values of only 4 to 29 kg N-fixed/ha (15 to 56% of plant N) were observed in semiarid southwestern Zimbabwe (4). In such areas, the application of a rhizobial inoculant may thus hold the potential to increase plant nutrition and soil fertility. Many effective rhizobial strains have been identif...

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Section: Isolation Of Root Nodule Rhizobia From the Okavango Regionmentioning

confidence: 62%

Rhizobia Indigenous to the Okavango Region in Sub-Saharan Africa: Diversity, Adaptations, and Host Specificity

Grönemeyer

Kulkarni

Hölscher

et al. 2014

Appl Environ Microbiol

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“…Subcultures in MM were performed as described previously (17). Antibiotics (in micrograms per milliliter) were added as follows: nalidixic acid, 20; streptomycin, 200; rifampin, 100; neomycin, 60; tetracycline, 10; fosfomycin, 200; cycloheximide, 10.…”

Section: Methodsmentioning

confidence: 99%

“…Phaseolus spp. are promiscuous plants that can be effectively nodulated by several rhizobial species, albeit with diverse nitrogen fixation efficiencies (1,(10)(11)(12)(13). Conversely, rhizobial bacteria have also been isolated from the tissues of nonleguminous plants, such as wheat (14) and rice (15) roots, maize stems (16), and Arabidopsis seeds (4).…”

mentioning

confidence: 99%

Nitrogen-Fixing Rhizobial Strains Isolated from Common Bean Seeds: Phylogeny, Physiology, and Genome Analysis

Mora

Dı́az

Vargas-Lagunas

et al. 2014

Appl Environ Microbiol

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Rhizobial bacteria are commonly found in soil but also establish symbiotic relationships with legumes, inhabiting the root nodules, where they fix nitrogen. Endophytic rhizobia have also been reported in the roots and stems of legumes and other plants. We isolated several rhizobial strains from the nodules of noninoculated bean plants and looked for their provenance in the interiors of the seeds. Nine isolates were obtained, covering most known bean symbiont species, which belong to the Rhizobium and Sinorhizobium groups. The strains showed several large plasmids, except for a Sinorhizobium americanum isolate. Two strains, one Rhizobium phaseoli and one S. americanum strain, were thoroughly characterized. Optimal symbiotic performance was observed for both of these strains. The S. americanum strain showed biotin prototrophy when subcultured, as well as high pyruvate dehydrogenase (PDH) activity, both of which are key factors in maintaining optimal growth. The R. phaseoli strain was a biotin auxotroph, did not grow when subcultured, accumulated a large amount of poly-␤-hydroxybutyrate, and exhibited low PDH activity. The physiology and genomes of these strains showed features that may have resulted from their lifestyle inside the seeds: stress sensitivity, a ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) complex, a homocitrate synthase (usually present only in free-living diazotrophs), a hydrogenase uptake cluster, and the presence of prophages. We propose that colonization by rhizobia and their presence in Phaseolus seeds may be part of a persistence mechanism that helps to retain and disperse rhizobial strains. Bacteria can populate diverse environments, from soil to water, even in the most extreme sites on the earth. These organisms can also be found on and inside other organisms, such as fungi, animals, and plants. The microbiome is a recently developed concept used to define the prokaryotic populations in close relationship with more-complex organisms (1, 2). Bacteria can flourish as endophytes inside plant roots, stems, leaves, and seeds (3) in crops (e.g., wheat, rice, maize, sorghum, and sugarcane), legumes (e.g., clover, common bean, and alfalfa), trees (e.g., Populus), grasses (e.g., switchgrass), and model plants (e.g., Arabidopsis) (4). Most common endophytic bacterial species have been described as members of the phyla Firmicutes and Proteobacteria (5).Legumes are economically valuable plants in agriculture and establish symbiotic relationships with rhizobial bacteria (6). These bacteria contribute to the formation of plant root nodules, colonizing them and fixing atmospheric nitrogen. This phenomenon has been studied intensively (7). Endophytic rhizobia, in tissues other than nodules, have also been isolated from clover and pea (e.g., Rhizobium phaseoli and Rhizobium leguminosarum bv. trifolii) (8). Several nonrhizobial bacterial species have been isolated from Phaseolus tissues (roots, stems, and seeds); these include Acinetobacter, Bacillus, Enterococcus, Nocardioides, Paracoccus, Phyll...

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“…It may be seen between species, as described for the nodulation of soybean by either Bradyrhizobium japonicum or B. elkani (e.g. Caldwell and Vest 1968) or between strains belonging to different genera, such as for the more promiscuous Phaseolus vulgaris (common bean) which may be nodulated by at least twenty-five species of nodule bacteria including seven species of Ensifer, four of Mesorhizobium, nine of Rhizobium, four of Bradyrhizobium and one species of Burkholderia (Michiels et al 1998;MartinezRomero 2003;Lindstrom et al 2010;Talbi et al 2010). However, in agricultural settings, the 'rhizobial competition problem' is most commonly at the interstrain level.…”

Section: "Competition" Between Rhizobia For Nodulationmentioning

confidence: 98%

A re-appraisal of the biology and terminology describing rhizobial strain success in nodule occupancy of legumes in agriculture

et al. 2011

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Aim To ensure that the majority of nodules on a legume are occupied by the inoculant strain poses a significant challenge to maximising nitrogen fixation in agricultural settings. The aim of this review is to establish a set of common terms with which to classify and interrogate experiments that investigate nodule occupancy in legumes. Results At least six different terminologies have been developed to discuss experimental outcomes where legumes have access to more than one rhizobial strain. In this review we nominate a set of preferred terms: promiscuous, exclusive, selective and non-selective nodulation in an attempt to provide conformity to the general scientific pursuit of understanding the circumstances leading to nodule occupancy in legumes when more than one strain has the opportunity to form the nodule. Discussion To maximise nitrogen fixation from legumes, a primary challenge is to prevent rhizobia resident in the soil from dominating nodule occupation, since whilst they may be numerically dominant, they are often not optimally matched for nitrogen fixation with the introduced legume. It is apparent, however, that at times the legume host is able to preferentially enter into symbiosis with a fully effective strain, even if an ineffective strain is present and numerically dominant. Conclusion This manuscript reviews the terminology currently applied to nodulation outcomes in introduced legume symbioses where more than one strain type is available to infect the plant. We suggest adoption of a more stringent terminology as the research discipline matures to exploit genome sequence information. We have suggested the term "selective nodulation" to describe certain nodulation outcomes that are not based upon numerical supremacy.

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Phaseolus vulgaris is a non-selective host for nodulation

Cited by 126 publications

References 28 publications

Rhizobia Indigenous to the Okavango Region in Sub-Saharan Africa: Diversity, Adaptations, and Host Specificity

Rhizobia Indigenous to the Okavango Region in Sub-Saharan Africa: Diversity, Adaptations, and Host Specificity

Nitrogen-Fixing Rhizobial Strains Isolated from Common Bean Seeds: Phylogeny, Physiology, and Genome Analysis

A re-appraisal of the biology and terminology describing rhizobial strain success in nodule occupancy of legumes in agriculture

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