Phaseolus vulgaris — Recalcitrant potential

Hnatuszko-Konka, Katarzyna; Kowalczyk, Tomasz; Gerszberg, Aneta; Wiktorek-Smagur, Aneta; Kononowicz, Andrzej K.

doi:10.1016/j.biotechadv.2014.06.001

Cited by 45 publications

(33 citation statements)

References 75 publications

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“…Unfortunately, the application of technologies based on plant transformation has been very slow in common bean; partly due to its relatively low efficiency for Agrobacterium tumefaciens-mediated transformation, which can be remedied by careful choice of A. tumefaciens strain (Mukeshimana et al, 2013), but most critically due to the recalcitrance of this plant to in vitro regeneration (Hnatuszko-Konka et al, 2014). The only successful study of which we are aware in which a transgenic approach has been used in common bean to generate resistance to a virus is for the Begomovirus Bean golden mosaic virus (BGMV) (Bonfim et al, 2007).…”

Section: Genetic Resistance and Future Potential For Its Exploitationmentioning

confidence: 99%

Bean Common Mosaic Virus and Bean Common Mosaic Necrosis Virus

Worrall

Wamonje

Mukeshimana

et al. 2015

Advances in Virus Research

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Section: Genetic Resistance and Future Potential For Its Exploitationmentioning

confidence: 99%

Bean Common Mosaic Virus and Bean Common Mosaic Necrosis Virus

Worrall

Wamonje

Mukeshimana

et al. 2015

Advances in Virus Research

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“…The common bean (Phaseolus vulgaris L.) is the most important legume for human consumption in several nations of Latin America, Africa, and Asia (Hnatuszko-Konka et al, 2014). It is an excellent source of protein, carbohydrates, and minerals such as iron and zinc, in addition to other nutrients (Gepts et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Diallel analysis to choose parents for black bean (Phaseolus vulgaris L.) breeding

Moura¹,

Carneiro²,

Vale

et al. 2016

Genet. Mol. Res.

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ABSTRACT. In this study, conducted in two different seasons, we aimed to choose parents to obtain promising segregating populations for the extraction of black bean (Phaseolus vulgaris L.) lines that are superior in terms of disease resistance, plant architecture, and grain yield. Twelve parents were arranged in two groups to compose a partial diallel in a 5 x 7 scheme. Group 1 was composed of parents with black grains and erect plant architecture, while group 2 was composed of parents that had carioca grains and were resistant to the main fungal diseases that occur in the common bean. The following traits were evaluated: severity of angular leaf spot (ALS), plant architecture (PAG), and grain yield (YIELD). The data were analyzed according to a partial diallel model using parents and F 1 hybrids. In the genetic control of ALS and PAG, additive effects were predominant, while for YIELD, additive effects were predominant in one season and dominance effects were in another season, because it is a more complex trait than ALS and PAG. For YIELD, we observed an interaction between general combining ability and specific combining ability between seasons. The genes that control ALS, PAG, and YIELD were in eight of the 12 parents evaluated in the diallel. The cultivar 'BRS Estilo' is suitable to use as a parent in common bean breeding in terms of ALS, PAG and YIELD. Recurrent selection is the most recommended option for simultaneously breeding for PAG, YIELD, and resistance to angular leaf spot in bean culture.

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“…As it is known from biotechnological research data, it is not the only species that displays troublesome recalcitrance towards genetic manipulations (e.g. Phaseolus vulgaris ; Brassica napus ) (Hnatuszko-Konka et al 2014; Liu et al 2015). As in the case of other important crops, the establishment of a reliable procedure of transformation would provide the space for their improvement or biopharming.…”

Section: Discussionmentioning

confidence: 99%

High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes

et al. 2018

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Brassica oleracea var. botrytis, a very popular crop grown for its edible inflorescence, is bred only as a mutated annual cultivar and does not naturally occur in environment. Since cauliflower is still described as the most troublesome of all the B. oleracea vegetables regarding transformation processes, it is fully justified to focus on the improvement of tools for its genetic modifications. Here, we present a successful protocol for genetic transformation of cauliflower employing the process of agroinfection. The primary analysis of in vitro response of five cultivars allowed us to have chosen Pionier as the most promising cultivar; in consequence the Pionier was transformed via Rhizobium-mediated techniques in order to evaluate both, R. radiobacter (EHA 105, LBA 4404) and R. rhizogenes (ATCC 18534, A4) species. However, the latter system turned out to be more effective and, the A4 strain, in particular (72% transformation efficiency, 55% confirmed by GUS assay). That shows a promising technical advance especially when compared to the results of previous literature reports (e.g. 8.7% reported efficiency using R. rhizogenes). The transgenic cauliflower was obtained from hairy roots via organogenic callus induction. The potential transformants were analysed at the genomic and proteomic levels and their transgenic character was fully confirmed.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0656-6) contains supplementary material, which is available to authorized users.

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Phaseolus vulgaris — Recalcitrant potential

Cited by 45 publications

References 75 publications

Bean Common Mosaic Virus and Bean Common Mosaic Necrosis Virus

Bean Common Mosaic Virus and Bean Common Mosaic Necrosis Virus

Diallel analysis to choose parents for black bean (Phaseolus vulgaris L.) breeding

High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes

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