In this study, eco-friendly superhydrophobic (SH) and superoleophilic (SO) poly (3-hydroxybuthyrate) (PHB)-SiO 2 bio-nanofiber membrane has been successfully fabricated for oil-water separation. Firstly, silica nanoparticles (SiNPs) have been synthesized via green laser ablation technique. It was placed into PHB matrix in different contents by controlling the time of ablation and then PHB-SiO 2 nanofibrous mats were obtained on commercial stainless-steel mesh with various mesh