PHB production from food waste hydrolysates by Halomonas bluephagenesis Harboring PHB operon linked with an essential gene

Ji, Mengke; Zheng, Thomas Fang; Wang, Ziyu; Lai, Weijian; Zhang, Lizhan; Yang, Hairui; Meng, Shaoping; Xu, Wen-Tao; Zhao, Cuihuan; Wu, Qiong; Chen, Guo‐Qiang

doi:10.1016/j.ymben.2023.03.003

Cited by 19 publications

(2 citation statements)

References 31 publications

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“…For example, Pseudomonas halophila has been used to produce biodegradable plastics such as PHA and PHB under nonsterile environments in the presence of seawater and high osmotic pressure. This not only reduces the cost of the water used in the fermentation system but also protects the limited freshwater resources on the planet. − Similarly, acidophilic bacteria show strong tolerance to the DCAs produced in the fermentation system. Further, their use is also conducive to the simplification of downstream separation and extraction processes and reduces sewage treatment under low pH conditions.…”

Section: Prospecting the Synthesis Of Dicarboxylic Acids From The Per...mentioning

confidence: 99%

Mid–Long Chain Dicarboxylic Acid Production via Systems Metabolic Engineering: Progress and Prospects

Gu,

Zhu,

Zhang

et al. 2024

J. Agric. Food Chem.

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Mid-to-long-chain dicarboxylic acids (DCA i , i ≥ 6) are organic compounds in which two carboxylic acid functional groups are present at the terminal position of the carbon chain. These acids find important applications as structural components and intermediates across various industrial sectors, including organic compound synthesis, food production, pharmaceutical development, and agricultural manufacturing. However, conventional petroleum-based DCA production methods cause environmental pollution, making sustainable development challenging. Hence, the demand for eco-friendly processes and renewable raw materials for DCA production is rising. Owing to advances in systems metabolic engineering, new tools from systems biology, synthetic biology, and evolutionary engineering can now be used for the sustainable production of energy-dense biofuels. Here, we explore systems metabolic engineering strategies for DCA synthesis in various chassis via the conversion of different raw materials into mid-to-long-chain DCAs. Subsequently, we discuss the future challenges in this field and propose synthetic biology approaches for the efficient production and successful commercialization of these acids.

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Section: Prospecting the Synthesis Of Dicarboxylic Acids From The Per...mentioning

confidence: 99%

Mid–Long Chain Dicarboxylic Acid Production via Systems Metabolic Engineering: Progress and Prospects

Gu,

Zhu,

Zhang

et al. 2024

J. Agric. Food Chem.

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“…Based on the development of next generation industrial biotechnology (Chen et al., 2020 ), H. bluephagenesis uses non-sterile culture to reduce the production cost of PHA. To further reduce the cost of producing PHA for H. bluephagenesis , researchers have explored low-cost substrate fermentation models utilizing substrates such as xylose (Tan et al., 2022 ), starch (Lin et al., 2021 ; Liu et al., 2024 ), and food waste (Ji et al., 2023 ), with notable success. Inexpensive corn steep powder (CSP), a by-product rich in amino acids, microbes, and mineral elements from starch processing, has been demonstrated to serve effectively in culturing H. bluephagenesis , substituting for costlier nitrogen sources such as yeast extract and peptone (Ye et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Unlocking growth potential in Halomonas bluephagenesis for enhanced PHA production with sulfate ions

Yao,

Yuan,

Zhou

et al. 2024

Journal of Industrial Microbiology and Biotechnology

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The mutant strain H. bluephagenesis (TDH4A1B5P) was found to produce PHA under low-salt, non-sterile conditions, but the yield was low. To improve the yield, different nitrogen sources were tested. It was discovered that urea was the most effective nitrogen source for promoting growth during the stable stage, while ammonium sulfate was used during the logarithmic stage. The growth time of H. bluephagenesis (TDH4A1B5P) and its PHA content were significantly prolonged by the presence of sulfate ions. After 64 hours in a 5-liter bioreactor supplemented with sulfate ions, the dry cell weight of H. bluephagenesis weighed 132 g/l and had a PHA content of 82%. To promote the growth and PHA accumulation of H. bluephagenesis (TDH4A1B5P), a feeding regimen supplemented with nitrogen sources and sulfate ions with ammonium sodium sulfate was established in this study. The dry cell weight was 124 g/L, and the PHA content accounted for 82.3% (w/w) of the dry cell weight, resulting in a PHA yield of 101 g/L in a 30-liter bioreactor using the optimized culture strategy. In conclusion, stimulating H. bluephagenesis (TDH4A1B5P) to produce PHA is a feasible and suitable strategy for all H. bluephagenesis.

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Metabolic modeling of Halomonas campaniensis improves polyhydroxybutyrate production under nitrogen limitation

Deantas-Jahn,

Mendoza,

Licona-Cassani

et al. 2024

Appl Microbiol Biotechnol

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Poly-hydroxybutyrate (PHB) is an environmentally friendly alternative for conventional fossil fuel-based plastics that is produced by various microorganisms. Large-scale PHB production is challenging due to the comparatively higher biomanufacturing costs. A PHB overproducer is the haloalkaliphilic bacterium Halomonas campaniensis, which has low nutritional requirements and can grow in cultures with high salt concentrations, rendering it resistant to contamination. Despite its virtues, the metabolic capabilities of H. campaniensis as well as the limitations hindering higher PHB production remain poorly studied. To address this limitation, we present HaloGEM, the first high-quality genome-scale metabolic network reconstruction, which encompasses 888 genes, 1528 reactions (1257 gene-associated), and 1274 metabolites. HaloGEM not only displays excellent agreement with previous growth data and experiments from this study, but it also revealed nitrogen as a limiting nutrient when growing aerobically under high salt concentrations using glucose as carbon source. Among different nitrogen source mixtures for optimal growth, HaloGEM predicted glutamate and arginine as a promising mixture producing increases of 54.2% and 153.4% in the biomass yield and PHB titer, respectively. Furthermore, the model was used to predict genetic interventions for increasing PHB yield, which were consistent with the rationale of previously reported strategies. Overall, the presented reconstruction advances our understanding of the metabolic capabilities of H. campaniensis for rationally engineering this next-generation industrial biotechnology platform. Key points A comprehensive genome-scale metabolic reconstruction of H. campaniensis was developed. Experiments and simulations predict N limitation in minimal media under aerobiosis. In silico media design increased experimental biomass yield and PHB titer.

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PHB production from food waste hydrolysates by Halomonas bluephagenesis Harboring PHB operon linked with an essential gene

Cited by 19 publications

References 31 publications

Mid–Long Chain Dicarboxylic Acid Production via Systems Metabolic Engineering: Progress and Prospects

Mid–Long Chain Dicarboxylic Acid Production via Systems Metabolic Engineering: Progress and Prospects

Unlocking growth potential in Halomonas bluephagenesis for enhanced PHA production with sulfate ions

Metabolic modeling of Halomonas campaniensis improves polyhydroxybutyrate production under nitrogen limitation

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