PHEMA/PPy cytocompatible conductive cryogels: One-pot synthesis, characterization, and electrical properties

Şarkaya, Koray; Çadırcı, Musa; Çetin, Kemal; Mutlu, Doğukan; Arslan, Şevki

doi:10.1016/j.mtcomm.2023.105791

Cited by 5 publications

References 87 publications

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Synthesis and characterization of polysaccharide-cryogel and its application to the electrochemical detection of DNA

Tunca,

Maral,

Yildiz

et al. 2024

Microchim Acta

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The main goal of our study is to demonstrate the applicability of the PPy-cryogel-modified electrodes for electrochemical detection of DNA. First, a polysaccharide-based cryogel was synthesized. This cryogel was then used as a template for chemical polypyrrole synthesis. This prepared polysaccharide-based conductive cryogel was used for electrochemical biosensing on DNA. Carrageenan (CG) and sodium alginate (SA) polysaccharides, which stand out as biocompatible materials, were used in cryogel synthesis. Electron transfer was accelerated by polypyrrole (PPy) synthesized in cryogel networks. A 2B pencil graphite electrode with a diameter of 2.00 mm was used as a working electrode. The prepared polysaccharide solution was dropped onto a working electrode as a support material to improve the immobilization capacity of biomolecules and frozen to complete the cryogelation step. PPy synthesis was performed on the electrodes whose cryogelation process was completed. In addition, the structures of cryogels synthesized on the electrode surface were characterized by thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). Surface characterization of the modified electrodes was performed by energy-dispersive X-ray spectroscopy (EDX) analysis. Electrochemical determination of fish sperm DNA (fsDNA) was performed using a PPy-cryogel-modified electrode. The use of a porous 3D cryogel intermediate material enhanced the signal by providing a large surface area for the synthesis of PPy and increasing the biomolecule immobilization capacity. The detection limit was 0.98 µg mL−1 in the fsDNA concentration range 2.5–20 µg mL−1. The sensitivity of the DNA biosensor was estimated to 14.8 µA mM−1 cm−2. The stability of the biosensor under certain storage conditions was examined and observed to remain 66.95% up to 45 days. Graphical Abstract

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Synthesis and characterization of polysaccharide-cryogel and its application to the electrochemical detection of DNA

Tunca,

Maral,

Yildiz

et al. 2024

Microchim Acta

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Electrically Conductive Polypyrrole@keratin Nanofiber Membranes with Potential for Tissue Restoration

Ma,

Lei,

Yang

et al. 2024

Fibers Polym

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Development and characterization of 3D-printed electroconductive pHEMA-co-MAA NP-laden hydrogels for tissue engineering

De Nitto,

Serafin,

Karadimou

et al. 2024

Bio-des. Manuf.

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Tissue engineering (TE) continues to be widely explored as a potential solution to meet critical clinical needs for diseased tissue replacement and tissue regeneration. In this study, we developed a poly(2-hydroxyethyl methacrylate-co-methacrylic acid) (pHEMA-co-MAA) based hydrogel loaded with newly synthesized conductive poly(3,4-ethylene-dioxythiophene) (PEDOT) and polypyrrole (PPy) nanoparticles (NPs), and subsequently processed these hydrogels into tissue engineered constructs via three-dimensional (3D) printing. The presence of the NPs was critical as they altered the rheological properties during printing. However, all samples exhibited suitable shear thinning properties, allowing for the development of an optimized processing window for 3D printing. Samples were 3D printed into pre-determined disk-shaped configurations of 2 and 10 mm in height and diameter, respectively. We observed that the NPs disrupted the gel crosslinking efficiencies, leading to shorter degradation times and compressive mechanical properties ranging between 450 and 550 kPa. The conductivity of the printed hydrogels increased along with the NP concentration to (5.10±0.37)×10−7 S/cm. In vitro studies with cortical astrocyte cell cultures demonstrated that exposure to the pHEMA-co-MAA NP hydrogels yielded high cellular viability and proliferation rates. Finally, hydrogel antimicrobial studies with staphylococcus epidermidis bacteria revealed that the developed hydrogels affected bacterial growth. Taken together, these materials show promise for various TE strategies. Graphic abstract

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PHEMA/PPy cytocompatible conductive cryogels: One-pot synthesis, characterization, and electrical properties

Cited by 5 publications

References 87 publications

Synthesis and characterization of polysaccharide-cryogel and its application to the electrochemical detection of DNA

Synthesis and characterization of polysaccharide-cryogel and its application to the electrochemical detection of DNA

Electrically Conductive Polypyrrole@keratin Nanofiber Membranes with Potential for Tissue Restoration

Development and characterization of 3D-printed electroconductive pHEMA-co-MAA NP-laden hydrogels for tissue engineering

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