Phenanthrene desorption from soil in the presence of bacterial extracellular polymer: observations and model predictions of dynamic beheavior

Liu, Anbo; Ahn, Ik Sung; Mansfield, Christopher R.; Lion, Leonard W.; Shuler, Michael L.; Ghiorse, William C.

doi:10.1016/s0043-1354(00)00324-9

Cited by 45 publications

(19 citation statements)

References 44 publications

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“…The data were expected to show that the EPS biopolymer has many charged groups and polar groups that readily uptake organic materials, such as humic acids, dye, and organic pollutants [49][50][51]. These findings demonstrate the removal of chromophoric organic materials through the microgel phase resulting in a decrease of fluorescence intensity.…”

Section: Sequestering Of Organic Materials and Heavy Metalsmentioning

confidence: 94%

Role of microgel formation in scavenging of chromophoric dissolved organic matter and heavy metals in a river-sea system

Shiu

Lee

2017

Journal of Hazardous Materials

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We use riverine and marine dissolved organic carbon (DOC) polymers to examine their aggregation behavior, and to evaluate the roles of microgel formation in scavenging of chromophoric dissolved organic matter (CDOM) and heavy metals in a river-sea system. Our results indicate that riverine and marine microgels did not exhibit very much difference in size and self-assembly curve; however, the assembly effectiveness ([microgel]/DOC) of marine samples was much higher than riverine. Instead of concentration of DOC, other factors such as types and sources of DOC polymers may control the microgel abundance in aquatic environments. After filtering water samples (microgels removed), the CDOM and selected metals (Cu, Ni, Mn) in the filtrate were quantified. CDOM and metals were concurrently removed to an extent via DOC polymer re-aggregation, which also suggested that the microgels had sequestering capability in CDOM and metals. This finding provides an alternative route for CDOM and heavy metals removal from the water column. As such the process of re-aggregation into microgels should then be considered besides traditional phase partitioning in the assessment of the ecological risk and fate of hazardous materials.

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Section: Sequestering Of Organic Materials and Heavy Metalsmentioning

confidence: 94%

Role of microgel formation in scavenging of chromophoric dissolved organic matter and heavy metals in a river-sea system

Shiu

Lee

2017

Journal of Hazardous Materials

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“…As part of a long-term project to model the interaction of toxic trace metals with biofilm exopolymers in natural and engineered systems (Lion et al, 1988;Hsieh et al, 1994;Nelson et al, 1996), we have focused on attempting to predict the interactions of metals with the biofilm components. We have been especially interested in the biogenic exopolymer components of biofilms produced by Proteobacteria (Chen et al, 1995a, b;Nelson et al, 1995;Liu et al, 2001). Chen et al (1995b) tested the effects of several Proteobacterial exopolymers on lead and cadmium distribution coefficients during adsorption isotherm experiments on sand.…”

Section: Pseudomonas Putida G7 Eps As a Model For Metalbinding Mechanmentioning

confidence: 99%

Structure and carbohydrate analysis of the exopolysaccharide capsule of Pseudomonas putida G7

Kachlany

Levery

Kim

et al. 2001

Environmental Microbiology

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113

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The aromatic hydrocarbon-degrading bacterium, Pseudomonas putida G7, produces exopolymers of potential interest in biotechnological applications. These exopolymers have been shown to have significant metal-binding ability. To initiate the study of the metal-polymer interactions, we explored the physical and chemical nature of the P. putida G7 exopolysaccharide, a major component of the exopolymer. A capsular structure was observed by light microscopy surrounding both planktonic and attached cells in biofilms after immunofluorescence staining with polyclonal antiserum raised against planktonic cells. Further work with planktonic cells showed that the immunostained capsule remained associated with young (log phase) cells, whereas older (stationary phase) cells lost their capsular material to the external milieu. Visualization of frozen, hydrated stationary phase cells by cryo-field emission scanning electron microscopy (cryoFESEM) revealed highly preserved extracellular material. In contrast, conventional scanning electron microscopy (SEM) of stationary phase cells showed rope-like material that most probably results from dehydrated and collapsed exopolymer. Both capsular and released exopolymers were separated from cells, and the released extracellular polysaccharide (EPS) was purified. Deoxycholate-polyacrylamide gel electrophoresis (PAGE) and silver/alcian blue staining of the partially purified material showed that it contained both EPS and lipopolysaccharide (LPS). Further purification of the EPS using a differential solubilization technique to remove LPS yielded highly purified EPS. Gas chromatography-mass spectrometry revealed that the purified EPS contained the monosaccharides, glucose, rhamnose, ribose, N-acetylgalactosamine and glucuronic acid. The structural and chemical properties of the P. putida EPS described here increase our understanding of the mechanisms of toxic metal binding by this well-known Proteobacterium.

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“…), which induce changes in the interactions with the substances (pollutants) present in water. For instance, EPS determine the nature of the aggregates during the flocculation process when used multivalent metals, as Al 3+ [ 32 ], and enhance the adsorption capacity of species in solution as heavy metals [ 33 ] or organic compounds [ 34 , 35 ].…”

Section: Resultsmentioning

confidence: 99%

Supported Biofilms on Carbon–Oxide Composites for Nitrate Reduction in Agricultural Waste Water

et al. 2021

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Escherichia coli colonies were grown on different supports for the removal of nitrates from water. A carbon material and different commercial metal oxides, such as SiO2, TiO2 and Al2O3, and their corresponding carbon–metal oxide composites were studied. The physicochemical properties were analyzed by different techniques and the results were correlated with their performance in the denitrification process. Developed biofilms effectively adhere to the supports and always reach the complete reduction of nitrates to gaseous products. Nevertheless, faster processes occur when the biofilm is supported on mesoporous and non-acid materials (carbon and silica).

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Phenanthrene desorption from soil in the presence of bacterial extracellular polymer: observations and model predictions of dynamic beheavior

Cited by 45 publications

References 44 publications

Role of microgel formation in scavenging of chromophoric dissolved organic matter and heavy metals in a river-sea system

Role of microgel formation in scavenging of chromophoric dissolved organic matter and heavy metals in a river-sea system

Structure and carbohydrate analysis of the exopolysaccharide capsule of Pseudomonas putida G7

Supported Biofilms on Carbon–Oxide Composites for Nitrate Reduction in Agricultural Waste Water

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