Phenantroindolizidine Alkaloids and Their Cytotoxicity from the Leaves of Ficus septica.

Wu, Pei‐Lin; Rao, Koppaka V.; Su, Chia‐Hao; Kuoh, Cheng‐Sheng; Wu, Tong

doi:10.1002/chin.200313190

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“…Perceiving alkaloid profile of F. septica leaves is advantageous in chemotherapy agent, and anticancer drug discovery for it may serve as a leading compound. To date, various studies in regard to cytotoxic effect of F. septica leaves have not investigated the profile of its alkaloid compounds, despite its cytotoxic effect has been recognized (Wu et al, 2002). Based on previous positive results on ethyl acetate soluble fraction, thus the present study investigates the profile of alkaloids in the ethyl acetate fraction with water-soluble components (fraction A) and ethyl acetate fraction with ethanol-soluble components (fraction B) from ethanolic extract of F. septica leaves and their cytotoxic effect on human T47D breast cancer cell line.…”

Section: Research Articlementioning

confidence: 99%

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Cytotoxic Effect and Constituent Profile of Alkaloid Fractions from Ethanolic Extract of Ficus septica Burm. f. Leaves on T47D Breast Cancer Cells

Nugroho¹,

Akbar²,

Wiyani³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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The study aimed to investigate the profile of alkaloids in two ethyl acetate soluble fractions, namely fractions A and B from an ethanolic extract of Ficus septica leaves and cytotoxic effect on T47D breast cancer cells. Preparation of both fractions involved maceration of leaves with 70% (v/v) ethanol, filtration with Al 2 O 3 , precipitation with 0.1 N HCl, Mayer reagent, and 0.1 N NaOH, and also partition with ethyl acetate. Qualitative thin layer chromatography (TLC) was conducted to determine the profile of alkaloids in the two fractions, using alkaloid specific reagents such as Dragendorff, sodium nitrite, and Van Urk-Salkowski. Cytotoxic effects of both fractions on T47D cells were evaluated using MTT assay with a concentration series of 1.56; 3.12; 6.25; 12.5; 25 and 50 μg/mL. The TLC test showed that fractions A and B contained alkaloids with Rx values of 0.74 and 0.80 for fraction A and 0.74, 0.84, 0.92 for fraction B with regard to yohimbine using the mobile phase of n-buthanol:glacial acetic acid:distilled water (3:1:1 v/v/v). Moreover, an indole alkaloid was detected with Rx values of 0.80 and 0.84, respectively. Fractions A and B exhibited high cytotoxic effects on T47D cells with IC50 values of 2.57 and 2.73 µg/mL, respectively. In conclusion, overall the results of this study showed that fractions of Ficus septica contain alkaloids including indole alkaloid or its derivatives and possess a cytotoxic effect on T47D cells. This research supports the idea that alkaloids in F. septica have anticancer activity.

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Section: Research Articlementioning

confidence: 99%

“…Previous study reported that alkaloid compounds in Awar-awar (Ficus septica) are potential as a cytotoxic agent (Wu et al, 2002;Lansky et al, 2008). Ethanolic extract of Ficus septica Burm.…”

Section: Introductionmentioning

confidence: 99%