Phenetic, genetic diversity and symbiotic compatibility of rhizobial strains nodulating pigeon pea in Northern India

Abstract

Pigeon pea () is one of the most important legumes grown in the northern province of Uttar Pradesh, India. However, its productively in Uttar Pradesh is lower than the average yield of adjoining states. During the course of the present study, a survey of pigeon pea growing agricultural fields was carried out and it was found that 80% of plants were inadequately nodulated. The study was aimed to evaluate the pigeon pea symbiotic compatibility and nodulation efficiency of root nodulating bacteria isolated from various legumes, and to explore the phenetic and genetic diversity of rhizobial population nodulating pigeon pea growing in fields of Uttar Pradesh. Amongst all the 96 isolates, 40 isolates showed nodulation in pigeon pea. These 40 isolates were further characterized by phenotypic, biochemical and physiological tests. Intrinsic antibiotic resistance pattern was taken to generate similarity matrix revealing 10 phenons. The study shows that most of the isolates nodulating pigeon pea in this region were rapid growers. The dendrogram generated using the NTSYSpc software grouped RAPD patterns into 19 clusters. The high degree of phenetic and genetic diversity encountered is probably because of a history of mixed cropping of legumes. The assessment of diversity is a very important tool and can be used to improve the nodulation and quality of pigeon pea crop. It is also concluded that difference between phenetic and RAPD clustering pattern is an indication that rhizobial diversity of pigeon pea is not as yet completely understood and settled.

Symbiotic efficiency and genetic characterization of rhizobia and non rhizobial endophytes associated with cowpea grown in semi-arid tropics of Kenya

“…Lack of phenotypic and genotypic correlation in rhizobia isolates nodulating cowpeas in Indian soils has been reported before by Arora et al. [ 37 ]. According to Gratten et al.…”

“…However, phylogenetically not all the rhizobia strains were closely related. This suggests that rhizobia strains, from the study area, with the potential to nodulate cowpea are not restricted to a phylogenetic group [ 37 ]. Therefore, these strains might have divergently evolved to colonize root nodules of different cowpea genotypes but retaining their critical genes that code for the nodulation of cowpea.…”

Medicinal plants with phytotoxic activity harbour endophytic bacteria with plant growth inhibitory properties

“…The potential of secondary metabolite production by endophytic bacteria, and their biological activity especially those exclusive to their host plants was reported in earlier studies (Mehanni and Safwat 2010;Kusari et al 2012). Endophytic bacteria that colonize internal plant tissue and able to synthesize plant beneficial metabolites which improve nutrient mobilization and uptake are expected to play a significant role in lowinput sustainable agriculture (Arora et al 2018). …”

Genetic Profiling and Diversity of Some Promising Efficient Rhizobial Isolates on Faba Bean Plants

“…1). The ISSR primers achieved a rate of polymorphism ranged between 25% to 80% (Table 3) which successfully explains that ISSR primers are efficient in determining the genetic fingerprinting and discrimination of rhizobial isolates (Arora et al, 2018) due to their high ability to produce polymorphic loci.…”