Phenolic bioactives from developmental stages of highbush blueberry (Vaccinium corymbosum) for hyperglycemia management using in vitro models

Abstract

K. 2015. Phenolic bioactives from developmental stages of highbush blueberry (Vaccinium corymbosum) for hyperglycemia management using in vitro models. Can. J. Plant Sci. 95: 653Á662. Blueberry is a rich source of soluble phenolics as well as human health relevant antioxidants. Phenolic-linked bioactive functionality of blueberry for type 2 diabetes management was screened during fruit maturation, especially from green to ripening stages using in vitro assays. Green fruit showed the highest total soluble phenolic content, whereas all three developmental stages of blueberry fruit exhibited high total antioxidant activity. Overall, ripe fruit had higher a-amylase and a-glucosidase inhibitory activity than green or green/pink fruit, and showed significant potential to improve glucose metabolism through in vitro assays. High phenolic-linked antioxidant activity along with moderate to high a-amylase and a-glucosidase inhibitory activity in ripe blueberry indicated its potential relevance as part of diet-based prevention and management of early stages of hyperglycemia associated with development of type 2 diabetes. This in vitro screening study provides a biochemical rationale and dietary strategy to develop the right blueberry cultivar and stage of fruit development for further validation in animal and clinical studies.Key words: Antioxidant, bioactive, functionality, highbush blueberry, hyperglycemia, phenolic Cheplick, S., Sarkar, D., Bhowmik, P. et Shetty, K. 2015. De´termination des compose´s phe´noliques bioactifs issus des stades de de´veloppement du bleuet en corymbe (Vaccinium corymbosum) pour la prise en charge de l'hyperglyce´mie au moyen de mode`les in vitro. Can. J. Plant Sci. 95: 653Á662. Le bleuet est une riche source de compose´s phe´noliques solubles ainsi que d'antioxydants pre´sentant un inte´reˆt pour la sante´humaine. On a examine´la bioactivite´des compose´s phe´noliques du bleuet pour le diabe`te de type 2 pendant la maturation du fruit, particulie`rement du fruit vert au fruit muˆr en recourant ad es tests in vitro. Le fruit vert est celui qui pre´sente la plus forte concentration totale de compose´s phe´noliques solubles, alors que les trois stades de de´veloppement illustrent une forte activite´totale en tant qu'antioxydant. Dans l'ensemble, le fruit muˆr inhibe plus la a-amylase et la a-glucosidase que le fruit vert ou rose, et pourrait ame´liorer de fac¸on significative le me´tabolisme du glucose, comme le de´montrent les essais in vitro. La forte activite´anti-oxydation lie´e aux compose´s phe´noliques ainsi que l'inhibition moyenne a`e´leve´e de la a-amylase et de la a-glucosidase des bleuets muˆrs indiquent que ce fruit pourrait pre´senter de l'inte´reˆt à titre pre´ventif dans l'alimentation, mais aussi pour ge´rer les premiers stades de l'hyperglyce´mie associe´e au de´veloppement du diabe`te de type 2. Cette e´tude de controˆle in vitro fournit un motif biochimique et une strate´gie die´te´tique en vue de la cre´ation d'un cultivar approprie´de bleuet et de l'e´tablissement du stade ...

Changes in phenolic profiles and antioxidant activity in rabbiteye blueberries during ripening

“…(2015). [2,3,10] These differences are mainly related to the bioactive components in fruits. [2,10,21]…”

“…Several studies have previously reported that phenolic acid contributed significantly to the total antioxidant capacity. [2,3,10] The total phenolic content was positively correlated with ferulic acid, p-coumaric acid, and epicatechin, weaker correlation with catechin. The total flavonoid contents were positively correlated with catechin, ferulic acid, quercetin, rutin, and ellagic acid.…”

“…[16,21,32] Other studies have also reported a high correlation between phenolic acid concentration and the total antioxidant capacity. [2,3,10]…”

“…Blueberry (Vaccinium spp) is a widely consumed fruit worldwide owing in part to its richness in bioactive compounds and excellent antioxidant activity. [1][2][3] New blueberry cultivars had been bred and selected based on their bioactive compounds and antioxidant activity. [4] Anthocyanins, phenolic acids, and flavonoids are the main bioactive compounds of blueberry.…”

“…The high total antioxidant activity in mature fruits could be explained by the elevated amounts of anthocyanin. [1,3,5,11] The phenolic compound and flavonoid contents showed evident antioxidant capacity. All phenolic contents were highly correlated with total antioxidant capacity and reducing power.…”