Noncentrifugal sugar (NCS) is a type of unrefined sugar made by evaporating the juice of sugar cane without separating the molasses, thus it contains a variety bioactive components. This study compared the chemical properties and antioxidant activities of NCS extracts with different solvents, and the antioxidant and anti-inflammatory activities of NCS were evaluated. The ethyl acetate extract showed higher efficiency in active ingredients with the highest content of total phenols (28.80 mg/g) and flavonoids (92.21 mg/g). Caffeic acid, chlorogenic acid and protocatechuic acid were the main components of nine phenolic substances in ethyl acetate extract. Results showed that 100 μg/mL NCS phenolic extracts significantly reduced ROS levels by 78% in t-BHP-induced Caco-2 cell models. The levels of SOD and GSH-Px were significantly increased, and MDA content was noticeably decreased, which could almost eliminate the effect of t-BHP induced oxidative. The secretion of interleukin IL-6 was significantly decreased by 72%, with NO content and iNOS expression declining.