Phenolic Composition and Bioactivity of Lavandula pedunculata (Mill.) Cav. Samples from Different Geographical Origin

Abstract

The aim of this study was to characterize the phenolic composition and evaluate the bioactivity of several samples of Lavandula pedunculata (Mill.) Cav, and to compare aqueous and hydroethanolic extracts. Plant materials were obtained by growing some accessions (seed samples) of various wild populations from different regions of Portugal conserved at the Portuguese Genebank in Braga. Phenolic compounds were analised by HPLC-DAD-ESI/MSn, antioxidant potential through in vitro assays (DPPH radical scavenging activity, reducing power and inhibition of lipid peroxidation), cytotoxicity on tumor cells (MCF-7, NCI-H460, HeLa and HepG2) and non-tumor (PLP2) cells, anti-inflammatory activity in rat RAW 264.7 macrophages, by the ability to inhibit NO production and antimicrobial potential by the microdilution method with INT dye (iodonitrotetrazolium chloride). Thirteen compounds were identified, being salvianolic acid B, rosmarinic acid and luteolin-7-O-glucuronide, the main compounds present, with values ranging between 44.3–582, 50.9–550, and 24.36–101.5 mg/g extract, respectively. L. pedunculata aqueous extract revealed a higher antioxidant potential (EC50 values between 14 to 530 μg/mL), which could be related to its higher concentration in phenolic compounds; however, the hydroethanolic extract showed a higher anti-inflammatory (lower EC50 values than 124 μg/mL) potential and antiproliferative capacity (lower GI50 values than 34 μg/mL). Thus, this study highlights the bioactive effects of this species and opens up possibilities of uses in food and pharmaceutical formulations. However, there are potential differences in such properties according to geographical origin of plant material, as in general, the samples from Alentejo presented higher results in all the bioactivities, compared with Trás-os-Montes samples.

Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview

“…samples from various different geographical origins in Portugal, and compared their bioactive activities in aqueous and hydroethanolic extracts. The obtained results pointed out that the L. pedunculata hydroethanolic extract from Alentejo area exhibited highest anti-inflammatory activity in rat RAW 264.7 macrophages by inhibiting nitric oxide production [ 70 ].…”

“…For over four million years, flavonoids and other phenolic substances from medicinal herbs have been used or consumed by humans so as to live healthy and fight against undesirable diseases [ 1 , 22 , 51 ]. As a plant secondary metabolites, flavonoids and the other phenolics are found in several plant species, type and amount of the chemical components are vary depending on species and affecting by environmental factors i.e., mineral at the growth locality and geographic origin [ 70 ]. Nowadays, there is a significant increase in the number of research on potential of medicinal plant species for pharmaceutical and medical purposes focusing on natural phenolic compounds and flavonoids [ 1 , 4 , 9 , 16 , 18 , 51 , 63 , 71 , 73 , 104 ].…”

Phenolic compounds and bioactivity evaluation of aqueous and methanol extracts ofAllium mongolicumRegel

“…The usage of natural phytochemicals as dietary intervention has received a great deal of attention as a potentially important concept in therapeutic intervention and public health (Hwang et al, 2018). Natural phenolic compounds have been suggested to be one of the interesting secondary metabolites due to their outstanding effects on clinical disease (Lopes et al, 2018). It was indicated that the extracts that contains high amount of polyphenolic obtained from herbs had effects in modulating the activity of selected digestive enzymes, such as lipase, a-glucosidase, and angiotensin-converting enzyme (ACE) (Sakulnarmrat & Konczak, 2012).…”

Antioxidant, Anti‐inflammatory and Cytotoxic Activities of Polyphenols Extracted from Chroogomphus rutilus

“…Absorbance at 490 nm was measured by a microplate reader. The results were expressed as the extract concentration providing 50 % cytotoxicity (EC 50 ) . Estimated EC 50 values are presented as the mean values of three independent experiments.…”

“…Besides the polysaccharides, polyphenols are one of the most important secondary metabolites of edible mushrooms. Most of the available reports describe the polyphenols of edible mushrooms, which were highlighted as having antioxidant, antitumor and anti‐inflammatory activity . Nevertheless, to our knowledge, the polyphenols of Chroogomphus rutilus are less studied than the other edible mushrooms.…”