Phenolic composition and biological activities of the in vitro cultured endangered Eryngium viviparum J. Gay

Abstract

Eryngium viviparum is an endangered species that requires management efforts based on complementary ex situ conservation strategies, such as in vitro culture. This study was carried out to evaluate the phenolic composition and the antioxidant, cytotoxic, and antimicrobial properties of E. viviparum aerial and root parts obtained by this micropropagation technique. The HPLC-DAD-ESI/MS n analysis showed that phenolic compounds were more abundant in the root (102 ± 4 mg g −1 extract) than in the aerial part (40.6 ± 0.8 mg g −1 extract) of the plant. The major compound was trans rosmarinic acid, followed by trans 3-O-caffeoylquinic acid. The root extract also showed higher antioxidant activity, with a result close to that of trolox in the thiobarbituric acid reactive substances (TBARS) formation inhibition assay, and a moderate toxicity to lung (NCI-H460), breast (MCF-7) and liver (HepG2) tumour cells. It was also more effective than ketoconazole against Penicillium ochrochloron. In turn, the aerial part extract inhibited Salmonella typhimurium more effectively than ampicillin. This study highlights E. viviparum as an unexplored source of bioactive compounds with potential application in the food, pharmaceutical, and other industrial sectors. Consequently, it promotes the interest of conserving this endangered species.

Promising Preserving Agents from Sage and Basil: A Case Study with Yogurts

“…Therefore, it appears that the antioxidant capacity of the plant extracts is possibly related to the phenolic composition, as shown in Section 3.1.3 . It is important to note that Trolox is a pure compound, while plant extracts are a mixture of compounds, with or without bioactive properties, which could explain the lowest antioxidant activity of the extracts when compared to Trolox [ 33 ].…”

Hypoglycemic Effect of Two Mexican Medicinal Plants

“…Rosmarinic acid (4) was identified by comparing it with a commercial standard and it was noted as the most abundant in the extract. This important antioxidant has also been identified as the most abundant compound in a hydroalcoholic extract of E. viviparum [32] and the second one in an aqueous extract of E. cymosum [33]. On the other hand, flavonol glycosides have been isolated and identified in several species of Eryngium [31], while isoflavones are less common.…”

“…On the other hand, flavonol glycosides have been isolated and identified in several species of Eryngium [31], while isoflavones are less common. Ayuso et al reported, in 2020, the presence of three tectorigenin glycosides for the first time in the genus [32]. However, more chemical analysis is needed to identify this kind of flavonoids in E. longifolium.…”

Phytochemical profiling of spiny coriander (Eryngium foetidum L.) – A potential perennial spicing-culinary herb of eastern India

“…Due to bioactive compounds, several Eryngium spp. have been used to treat human physiological disorders [9,10,11]. Although reports on pharmaceutical applications of E. foetidum are available, phytochemical investigation of leaves and the branches of E. foetidum, in particular, are scanty [12,13,14].…”