Phenolic Composition of Grape Stems

Souquet, Jean‐Marc; Labarbe, Benoît; Guernevé, Christine Le; Cheynier, Véronique; Moutounet, Michel

doi:10.1021/jf991171u

Cited by 253 publications

(193 citation statements)

References 16 publications

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“…The polyphenols were fractionated on a Fractogel column by a method adapted from Souquet et al (14). Procyanidins were characterized and quantified by thiolysis coupled with reverse-phase HPLC and diode array UV-visible detection (15).…”

Section: Methodsmentioning

confidence: 99%

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Potentiation of apple procyanidin-triggered apoptosis by the polyamine oxidase inactivator MDL 72527 in human colon cancer-derived metastatic cells

Gossé

Roussi

Guyot³

et al. 2006

Int J Oncol

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Abstract. Apple procyanidins have chemopreventive properties in a model of colon cancer, they affect intracellular signalling pathways, and trigger apoptosis in a human adenocarcinoma-derived metastatic cell line (SW620). In the present study we investigated relationships between procyanidin-induced alterations in polyamine metabolism and apoptotic effects. Apple procyanidins diminish the activities of ornithine decarboxylase and S-adenosyl-Lmethionine decarboxylase, key enzymes of polyamine biosynthesis, and they induce spermidine/spermine N 1 -acetyltransferase, which initiates retroconversion of polyamines. As a consequence of the enzymatic changes polyamine concentrations are diminished, and N 1 -acetyl-polyamines accumulate in SW620 cells. In contrast with expectations MDL 72527, an inactivator of polyamine oxidase (PAO), improved the anti-proliferative effect of procyanidins, and caused an increase of the proportion of apoptotic cells, although it prevented the formation of hydrogen peroxide and 3-acetamidopropanal, the cytotoxic products of PAOcatalysed degradation of N 1 -acetylspermidine and N 1 -acetylspermine. Addition of 500 μM N 1 -acetylspermidine to the culture medium in the presence of procyanidins mimicked the effect of MDL 72527. Therefore we presume that the enhanced procyanidin-triggered apoptosis by MDL 72527 is mediated by the accumulation of N 1 -acetyl-polyamines. The observation that apple procyanidins enhance polyamine catabolism and reduce polyamine biosynthesis activity similar to known inducers of SSAT, without sharing their toxicity, and the potentiation of these effects by low concentrations of MDL 72527 suggests apple procyanidins for chemopreventive and therapeutic interventions.

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Section: Methodsmentioning

confidence: 99%

“…After centrifugation at 33,000 x g for 25 min at 4˚C, the supernatants were collected and ODC and AdoMetDC assays were performed. ODC activity was evaluated by measuring 14 Polyamine determinations. Cells were homogenized in perchloric acid (200 mM), and the homogenates were centrifuged at 1500 x g for 10 min after standing for 16 h at 2˚C.…”

Section: Methodsmentioning

confidence: 99%

Potentiation of apple procyanidin-triggered apoptosis by the polyamine oxidase inactivator MDL 72527 in human colon cancer-derived metastatic cells

Gossé

Roussi

Guyot³

et al. 2006

Int J Oncol

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“…Consistent with their role as important UV light screens, flavonols accumulate mainly in flowers and skins of grape berries, but they have also been reported in leaves and stems. No detectable amounts of flavonols have been identified in pulp and seeds of cv Chardonnay and Shiraz berries, and expression of VvFLS1 was also not detected in these tissues (Souquet et al, 2000;Downey et al, 2003b).…”

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confidence: 95%

The Grapevine R2R3-MYB Transcription Factor VvMYBF1 Regulates Flavonol Synthesis in Developing Grape Berries

et al. 2009

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Flavonols are important ultraviolet light protectants in many plants and contribute substantially to the quality and healthpromoting effects of fruits and derived plant products. To study the regulation of flavonol synthesis in fruit, we isolated and characterized the grapevine (Vitis vinifera 'Shiraz') R2R3-MYB transcription factor VvMYBF1. Transient reporter assays established VvMYBF1 to be a specific activator of flavonol synthase1 (VvFLS1) and several other promoters of grapevine and Arabidopsis (Arabidopsis thaliana) genes involved in flavonol synthesis. Expression of VvMYBF1 in the Arabidopsis mutant myb12 resulted in complementation of its flavonol-deficient phenotype and confirmed the function of VvMYBF1 as a transcriptional regulator of flavonol synthesis. Transcript analysis of VvMYBF1 throughout grape berry development revealed its expression during flowering and in skins of ripening berries, which correlates with the accumulation of flavonols and expression of VvFLS1. In addition to its developmental regulation, VvMYBF1 expression was light inducible, implicating VvMYBF1 in the control of VvFLS1 transcription. Sequence analysis of VvMYBF1 and VvFLS1 indicated conserved putative light regulatory units in promoters of both genes from different cultivars. By analysis of the VvMYBF1 amino acid sequence, we identified the previously described SG7 domain and an additional sequence motif conserved in several plant MYB factors. The described motifs have been used to identify MYB transcription factors from other plant species putatively involved in the regulation of flavonol biosynthesis. To our knowledge, this is the first functional characterization of a light-inducible MYB transcription factor controlling flavonol synthesis in fruit.

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“…(ALONSO et al, 2002;LOULI;RAGOUSSIS;MAGOULAS, 2004;KAMMERER et al, 2005;PINELO et al, 2005). Outras partes do conjunto da uva são rejeitadas durante o processo de vinificação, como os engaços, e recebem muito menos atenção, embora contenham uma quantidade importante de polifenóis (SOUQUET et al, 2000).…”

Section: Introductionunclassified

Influência do solvente no conteúdo total de polifenóis, antocianinas e atividade antioxidante de extratos de bagaço de uva (Vitis vinifera) variedades Tannat e Ancelota

Rockenbach¹,

Silva²,

Rodrigues³

et al. 2008

Ciênc. Tecnol. Aliment.

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É de conhecimento científico o potencial antioxidante dos compostos fenólicos, atuando como redutores de oxigênio singleto, nas reações de oxidação lipídica e na quelação de metais. Apresentam uma ampla gama de propriedades farmacológicas, como antialergênicas, antiarteriogênicas, antiinflamatórias, antimicrobianas, antitrombóticas e também efeitos cardioprotetores e vasodilatadores (PUUPPONEN-PIMIÄ et al., 2001;MANACH;MAZUR;SCALBERT, 2005).A uva é fonte de diversos compostos fenólicos em elevadas concentrações e os subprodutos da vinificação, em sua maioria, podem manter quantidades apreciáveis, principalmente de fenó-licos, que pertencem ao grupo dos flavonóides. Os glicosídeos de flavonóis e as antocianinas estão entre os compostos fenólicos mais determinados e estudados nas uvas, por sua destacada atividade antioxidante e por suas propriedades antiinflamatórias e anticancerígenas (NEGRO; TOMMASI; MICELI, 2003;AMICO et al., 2004;SILVA;MATIAS;NUNES, 2005).O processo de fabricação do vinho gera uma quantidade estimada de resíduo sólido de 20% do peso inicial ( GÓMEZ-PLAZA; MIÑANO; LÓPEZ-ROCA, 2006). Alguns estudos a respeito dos subprodutos da vinificação focalizam principalmente a composição de polifenóis das sementes, que são muito ricas em flavonóis (YILMAZ; TOLEDO, 2004; GUENDEZ et al., 2005b). As sementes representam em torno AbstractDifferent solvent systems were applied in order to determine the extraction efficiency of compounds with antioxidant capacity from grape bagasse, an important by-product from wine production. The quantification of total phenolics, anthocyanins, and antioxidant activity was carried out in grape bagasse extracts of Tannat and Ancelota varieties from the region of Videira, state of Santa Catarina. The antioxidant activity was determined by ABTS, FRAP, and β-carotene/linoleic acid system methods. Extracts from acidified aqueous solutions of acetone and ethanol in different concentrations (v/v) showed higher yield in aqueous solutions of acetone 50 and 70% for Tannat variety and acetone/ethanol 50% for Ancelota variety, respectively. The total phenolic contents in acetone 50 and 70% were higher in both varieties while the anthocyanin contents in these varieties were higher in ethanol 50 and 70%. The antioxidant activity was higher in acetone 50 and 70% for Tannat variety and acetone/ethanol 50 and 70% for Ancelota variety by the ABTS method. Regarding the reduction power performed by the FRAP method, it was higher in acetone 70% in both varieties. During the oxidation inhibition power assay, the addition of 100 and 200 µL of ethanolic extracts 50% of Tannat and Ancelota varieties showed higher efficiency since it was almost two times higher than acetonic extracts. Keywords: byproducts; extraction; oxidation; reduction power. ResumoDiferentes sistemas solventes foram aplicados para determinar a eficiência de extração de compostos com capacidade antioxidante em bagaço de uva, importante subproduto do processo de vinificação. Realizou-se a quantificação de compostos fenólicos totais,...

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Phenolic Composition of Grape Stems

Cited by 253 publications

References 16 publications

Potentiation of apple procyanidin-triggered apoptosis by the polyamine oxidase inactivator MDL 72527 in human colon cancer-derived metastatic cells

Potentiation of apple procyanidin-triggered apoptosis by the polyamine oxidase inactivator MDL 72527 in human colon cancer-derived metastatic cells

The Grapevine R2R3-MYB Transcription Factor VvMYBF1 Regulates Flavonol Synthesis in Developing Grape Berries

Influência do solvente no conteúdo total de polifenóis, antocianinas e atividade antioxidante de extratos de bagaço de uva (Vitis vinifera) variedades Tannat e Ancelota

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