Phenolic composition, organic acids, sugars, vitamin C and antioxidant activity in the juice of two new triploid late-season mandarins

Comparative Study of Bioactive Components and Quality from Juices of Different Mandarins: Discriminant Multivariate Analysis of Their Primary and Secondary Metabolites

“…The amounts of hesperidin varied between 25.75 and 12.94 mg•100 mL ). For didymin levels, the values shown in all the studied varieties agree with previous studies into tangor and mandarin fruits [18] [21] [29].…”

“…Total vitamin C was extracted according to the method described elsewhere [9], with some modifications to adapt the method to a microliter format [18], using DL-dithiothreitol (DTT) as reducing reagent of dehydroascorbic acid to ascorbic acid (ASC). Briefly, 1 mL of filtered juice was mixed with 1 mL of 5% metaphosphoric acid solution, and then the sample was centrifuged (Eppendorf 5810R centrifuge; Eppendorf Iberica, Madrid, Spain) at 4˚C for 15 min at 8000 ×g.…”

“…One mL of supernatant was mixed with 200 µL of DTT (20 mg•mL −1 ) and left to react for 2 h in the dark, then filtered through 0.45 µm filter. It was analyzed by HPLC-DAD in an Alliance liquid chromatographic system (Waters, Barcelona, Spain) equipped with a 2695 separation module coupled to a 2996 photodiode array detector, and a reverse-phase C 18 column with an isocratic mobile phase of methanol: 0.6% acetic acid (5:95, v/v), as previously described [18]. The Empower 2 software (Waters, Spain) was used for data processing, and quantification was performed at 245 nm by external standard calibration.…”

“…Then the sample was centrifuged (Eppendorf 5810R centrifuge; Eppendorf Iberica, Madrid, Spain) at 4˚C for 15 min at 8000 ×g. The supernatant was filtered through a 0.45 µm nylon filter and analyzed by HPLC-DAD and HPLC-MS in a reverse-phase column C 18 . An analysis was done using an Alliance liquid chromatographic system (Waters, Barcelona, Spain) equipped with a 2695 separation module, coupled to a 2996 photodiode array detector and a ZQ2000 mass detector.…”

Genetic Structure of Diploid Gametes for the Production of Triploid Citrus Hybrids

“…Citrus fruits have a multitude of health promoting properties and research has made significant contributions in connecting specific health benefits to a group of secondary metabolites (Butelli et al, 2012;2017). Otherwise, it´s important to obtain cultivars producing fruits during the whole harvesting season to avoid the need for conservation and in order to fulfill the demand in optimal conditions Sdiri et al, 2012;.…”