Phenolic compounds as marker compounds for botanical origin determination of German propolis samples based on TLC and TLC-MS

Bertrams, Julia; Kunz, Nadine; Müller, Melanie; Stintzing, Florian C.

doi:10.5073/jabfq.2013.086.020

Cited by 17 publications

(10 citation statements)

References 34 publications

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“…The HPTLC technique was previously used to characterize propolis samples by other authors. Polyphenol profiles of samples of different origins were compared to determine the type of propolis (O, B, and G-type) originating from Germany [ 11 ]. This technique was also used to determine the authenticity of European propolis samples, based on the characteristic marker compounds of the flavonoid group, e.g., naringenin, chrysin, galangin [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

“…Flavonoids, such as pinobanksin, pinocembrin, galangin, chrysin, kaempferol, and quercetin, are common constituents of poplar-type propolis [ 9 ]. The preliminary bioactivity screening of natural compounds in propolis could be achieved with using high-performance thin-layer chromatography (HPTLC), and high-performance liquid chromatography (HPLC) [ 10 , 11 , 12 , 13 ], which are simple and rapid techniques for the identification of bioactive compounds present in extracts.…”

Section: Introductionmentioning

confidence: 99%

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The Study of Chemical Profile and Antioxidant Properties of Poplar-Type Polish Propolis Considering Local Flora Diversity in Relation to Antibacterial and Anticancer Activities in Human Breast Cancer Cells

et al. 2022

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Nine samples of ethanolic extracts of poplar-type propolis (EEP) originated from South-Eastern Poland were analyzed in terms of the diversity of the flora around the apiary. The mineral composition, antioxidant properties, polyphenolic profile (HPTLC), and main polyphenolic constituents (HPLC-DAD) were determined. Only minor differences in chemical composition and antioxidant capacity between tested EEPs were found regardless of their botanical origin. However, the biological activity of the EEPs was more diversified. The tested EEPs showed stronger antibacterial activity against Gram-negative bacteria (Escherichia coli) compared to Gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis). Staphylococci biofilm inhibition occurred as a result of exposure to the action of four out of nine EEPs (P1–P4). Due to the various compositions of individual EEPs, a different MCF-7 cellular response was observed according to inhibition of cells migration and proliferation. Almost every sample inhibited the migration of breast cancer cells at a low concentration (0.04 µg/mL) of propolis. Even at the lowest concentration (0.02 µg/mL), each EEP inhibited the proliferation of MCF-7 cells, however, the level of inhibition varied between samples.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Study of Chemical Profile and Antioxidant Properties of Poplar-Type Polish Propolis Considering Local Flora Diversity in Relation to Antibacterial and Anticancer Activities in Human Breast Cancer Cells

et al. 2022

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“…All four markers of P. tremula were found to be phenolic acid glycerols, and (except for 2-acetyl-1,3-di-feruloyl glycerol ( 24 )) they have previously been reported in P. tremula leaf buds [ 5 ]. Marker 22 was identified as 2-acetyl-1,3-di- p -coumaroyl glycerol, and its MS/MS spectrum included fragment ions characteristic of coumaric acid at m/z 117, 119, 145, and 163.…”

Section: Resultsmentioning

confidence: 94%

“…The chemical diversity of plants and the special chemical features of different plant families, genera, and species provide exciting possibilities for their discrimination and chemotaxonomic classification. Such discrimination is useful for not only educational [ 1 ] and species identification purposes [ 2 ] but also quality control [ 3 , 4 , 5 ] as different types of plant-based natural products increase in their popularity. Depending on the need, know-how, and available equipment, different types and levels of tools are needed to execute these discriminative or chemotaxonomic actions.…”

Section: Introductionmentioning

confidence: 99%

“…For common Finnish deciduous trees, there is a wide range of structurally different compounds described in the scientific literature that could serve as chemotaxonomic markers. Populus tremula is a good example, with phenolic acid glycerols characteristic for its leaf buds [ 5 ], whereas anthocyanins have been suggested as chemotaxonomic markers for the anthers of different Populus species [ 9 ]. In addition, Abreu et al and Julkunen-Tiitto found that salicylate-like simple phenolic glucosides could serve as chemotaxonomic markers at the genus level for both Populus and Salix species [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Chemotaxonomic Markers for the Leaf Buds of Common Finnish Trees and Shrubs: A Rapid UHPLC MS Fingerprinting Tool for Species Identification

2022

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In this study, a chemotaxonomic tool was created on the basis of ultra-high-performance liquid chromatography–mass spectrometry (UHPLC–MS) for the identification of 13 common Finnish deciduous trees and shrubs from their leaf bud metabolites. The bud extracts were screened with UHPLC–ESI–QqQ–MS and UHPLC–ESI–Q–Orbitrap–MS to discover suitable markers for each species. Two approaches were tested in the marker selection: (1) unique species-specific markers to obtain selective fingerprints per species and (2) major markers to maximise the sensitivity of the fingerprints. The markers were used to create two selected ion-recording-based fingerprinting tools with UHPLC–ESI–QqQ–MS. The methods were evaluated for their selectivity, repeatability, and robustness in plant species identification by analysing leaf buds from several replicates of each species. The created chemotaxonomic tools were shown to provide unique chromatographic profiles for the studied species in less than 6 min. A variety of plant metabolites, such as flavonoids, triterpenoids, and hydroxycinnamic acid derivatives, were found to serve as good chemotaxonomic markers for the studied species. In 10 out of 13 cases, species-specific markers were superior in creating selective and repeatable fingerprints.

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Application High-Performance Thin-Layer Chromatography with Controlled Eluent Velocity to Determine Fingerprints of Various Poplar Species Buds Extracts: Optimization of Operating Variables

Hałka-Grysińska,

Polak,

Warianek

et al. 2024

Chromatographia

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The study used the prototype device enabling the development of isocratic and gradient thin-layer chromatograms with controlled eluent velocity to obtain fingerprints of extracts from buds of various poplar species. This equipment can deliver an eluent to virtually any place on the adsorbent layer of the chromatographic plate. Moreover, the composition of a mobile phase can be easily modified even when the chromatogram is developing. This paper shows the effect of various operating variables of the prototype in a normal phase system, e.g., eluent velocity and composition, on the chromatographic behavior of the mixture. The prototype’s results were compared to conventional TLC development in horizontal DS chambers. The UV light (366 nm) was used to detect the solute zones and document both systems. The results prove that the prototype device has great potential in plant material standardization. The mobile phase gradient improves the separation of the bands of strongly retained solutes. Additionally, this fact does not enhance the blurring of low-retained bands.

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Phenolic compounds as marker compounds for botanical origin determination of German propolis samples based on TLC and TLC-MS

Cited by 17 publications

References 34 publications

The Study of Chemical Profile and Antioxidant Properties of Poplar-Type Polish Propolis Considering Local Flora Diversity in Relation to Antibacterial and Anticancer Activities in Human Breast Cancer Cells

The Study of Chemical Profile and Antioxidant Properties of Poplar-Type Polish Propolis Considering Local Flora Diversity in Relation to Antibacterial and Anticancer Activities in Human Breast Cancer Cells

Chemotaxonomic Markers for the Leaf Buds of Common Finnish Trees and Shrubs: A Rapid UHPLC MS Fingerprinting Tool for Species Identification

Application High-Performance Thin-Layer Chromatography with Controlled Eluent Velocity to Determine Fingerprints of Various Poplar Species Buds Extracts: Optimization of Operating Variables

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