Phenolic constituents isolated from Senna tora sprouts and their neuroprotective effects against glutamate-induced oxidative stress in HT22 and R28 cells

Kwon, Jae‐Young; Hwang, Hoseong; Selvaraj, Baskar; Lee, Jung Hwan; Park, Woongbi; Ryu, Seung Mok; Lee, Dong‐Ho; Park, Jin-Soo; Kim, Hyoung Seok; Lee, Jae Woo; Jang, Dae Sik; Kwon, Hak Cheol

doi:10.1016/j.bioorg.2021.105112

Cited by 13 publications

(6 citation statements)

References 38 publications

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“…In our study, we found that NGR1 alone is not neurocytotoxic even at high concentrations. However, glutamate alone inhibited HT22 activity, promoted apoptosis, increased levels of LDH, and activated oxidative stress; this is consistent with the results in previous studies [ 26 ]. In addition, NGR1 pretreatment significantly protected HT22 cells against glutamate-induced neurocytotoxic by decreasing levels of LDH, inhibiting apoptosis, and oxidative stress.…”

Section: Discussionsupporting

confidence: 93%

Protective Effect of NGR1 against Glutamate-Induced Cytotoxicity in HT22 Hippocampal Neuronal Cells by Upregulating the SIRT1/Wnt/β-Catenin Pathway

Wang¹,

Gao

Yang³

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Notoginsenoside R1 (NGR1) is an active compound isolated from Panax notoginseng. Despite the NGR1 having been used as a traditional medicine, little is known about the neuroprotective effects. In this study, we investigate the protective effects of NGR1 against glutamate-induced cytotoxicity in HT22 cells and its possible molecular mechanism. We assessed the toxicity of NGR1 and the protective activity by MTT assay. The levels of oxidative stress indices superoxide dismutase (SOD), glutathione (GSH), and mitochondrial membrane potential (MMP) were measured by the kits. The levels of reactive oxygen species (ROS) and Ca2+ concentration were measured by flow cytometry. Furthermore, we determined the expression of mitochondrial dysfunction related protein PINK1, Parkin, silent mating type information regulation 2 homolog-1 (sirtuin 1; SIRT1), and Wnt/β-catenin by Western blotting. Here, we discovered that glutamate treatment led to cell viability loss, apoptosis facilitation, Ca2+ upregulation, MMP fluorescence intensity downregulation, and ROS generation of HT22 cells. In parallel, expression of Parkin was declined by glutamate. While, NGR1 treatment alleviated all the above phenomena. We further clarified that NGR1 alleviated glutamate-induced oxidative stress, apoptosis, and mitochondrial dysfunction by upregulating SIRT1 to activate Wnt/β-catenin pathways. These findings demonstrate that NGR1 alleviated glutamate-induced cell damage, and NGR1 may play a protective role in neurological complications.

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Section: Discussionsupporting

confidence: 93%

Protective Effect of NGR1 against Glutamate-Induced Cytotoxicity in HT22 Hippocampal Neuronal Cells by Upregulating the SIRT1/Wnt/β-Catenin Pathway

Wang¹,

Gao

Yang³

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…Computational analysis was performed with reference to the literature . Conformational analysis was conducted using Spartan′14 software (Wavefunction, Inc., Irvine, CA, USA) under the molecular mechanics force field.…”

Section: Methodsmentioning

confidence: 99%

Ecdysteroids from the Korean Endemic Species Ajuga spectabilis with Activities against Glucocorticoid Receptors and 11β-Hydroxysteroid Dehydrogenase Type 1

Park

Lee

et al. 2023

ACS Omega

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Two new ecdysteroids, spectasterone A (1) and spectasterone B (2), together with four known ecdysteroids, breviflorasterone (3), ajugalactone (4), 20-hydroxyecdysone (5), and polypodine B (6) were isolated from the Korean endemic plant Ajuga spectabilis using feature-based molecular networking analysis. The chemical structures of 1 and 2 were determined based on the interpretation of NMR and mass spectrometric data. Their absolute configurations were established using 3 J H, H coupling constants, NOESY interactions, Mosher’s method, and ECD and DP4+ calculations. To identify their biological target, a machine learning-based prediction system was applied, and the results indicated that ecdysteroids may have 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1)-related activity, which was further supported by molecular docking results of ecdysteroids with 11β-HSD1. Following this result, all the isolated ecdysteroids were tested for their ability to affect the expression of 11β-hydroxysteroid dehydrogenase type 1 and glucocorticoid receptors (GRs) in HaCaT cells irradiated with UVB. Compounds 2–5 exhibited inhibition of 11β-HSD1 expression and increases in GR activity.

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“…To identify these antioxidative compounds, various chromatographic and spectroscopic methods were carried out for the isolation and structural characterization of the compounds. A new flavonoid (1) and a new natural compound (3) were obtained from the ethyl acetate layer of the ethanol extract, together with 25 previously reported compounds, namely kaempferol-3-O-β-2"-coumaroylglucoside (2) [16], (+)-catechin (4) [17], hyperoside (5) [17], isoquercitrin (6) [18], quercetin-3-O-β-2"-galloylgalactoside (7) [19], quercetin-3-O-β-2"-galloylglucoside (8) [20], trifolin (9) [18], astragalin (10) [18], kaempferol-3-O-β-2"-galloylgalactoside (11) [21], kaempferol-3-O-α-arabinoside (12) [22], kaempferol-3-O-β-2"-galloylglucoside (13) [23], quercetin-3-O-β-2"-coumaroylglucoside ( 14) [24], quercetin (15) [15], kaempferol (16) [25], (6S,9S)-roseoside (17) [26], scopoletin (18) [27], umbelliferone (19) [28], 1-(2,4-dihydroxy-6-methylphenyl)ethanone (20) [29], barbinervic acid (21) [30], diospyric acid B (22) [7], rotungenic acid (23) [31], pomolic acid (24) [32], siaresinolic acid (25) [33], oleanolic acid…”

Section: Phytochemical Investigationmentioning

confidence: 99%

“…The contents of flavonoids were approximately 5.4%, and of these, those of active compounds were 3.2% (Table 2). In particular, the contents of kaempferol-3-O-β-2 -galloylgalactoside (9), kaempferol-3-O-β-2"-galloylglucoside (10), isoquercitrin (13), and quercetin-3-O-β-2"galloylglucoside (15) were more than 0.3%. The analytical method was verified using a simple validation procedure to ensure the relevance of the method, which showed adequate specificity, linearity, accuracy, and precision.…”

Section: Quantitative Analysis Of Isolated Compoundsmentioning

confidence: 99%

“…As part of our continuous project to find biologically active compounds [15], the antioxidative effect of the ethanol (EtOH) extract and solvent partitions from the persimmon leaves was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and online highperformance liquid chromatography-2,2 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (HPLC-ABTS) analysis. A phytochemical study on the persimmon leaves led to the isolation of one new flavonoid (1) and one new natural compound (3), along with 25 previously known compounds.…”

Section: Introductionmentioning

confidence: 99%

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Chemical Constituents of the Leaves of Diospyros kaki (Persimmon)

Kwon

Park

Lee

et al. 2021

Plants

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Diospyros kaki (persimmon) leaves have long been utilized as traditional medicine for the treatment of ischemic stroke, angina, and hypertension and as a healthy beverage and cosmetic for anti-aging. This study aimed to isolate as many compounds as possible from an ethanol extract of the persimmon leaves to identify the biologically active compounds. The antioxidative effect of the ethyl acetate layer from the ethanol extract of the persimmon leaves was demonstrated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and online high-performance liquid chromatography-2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (HPLC-ABTS) analysis. A new flavonoid, kaempferol-3-O-β-d-2″-coumaroylgalactoside (1), and a new natural compound, kaempferol-3-O-β-d-2″-feruloylglucoside (3) were isolated from the ethyl acetate layer, along with 25 previously known compounds, including fourteen flavonoids, one ionone, two coumarins, seven triterpenoids, and one acetophenone. Their structures were determined by the interpretation of spectrometric and spectroscopic data. All isolated compounds were rapidly evaluated using an online HPLC-ABTS assay, and of these, compounds 4–8, 11, 13, 15, and 16 clearly showed antioxidative effects. The amount of these compounds was 0.3–0.65% of the extract.

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Phenolic constituents isolated from Senna tora sprouts and their neuroprotective effects against glutamate-induced oxidative stress in HT22 and R28 cells

Cited by 13 publications

References 38 publications

Protective Effect of NGR1 against Glutamate-Induced Cytotoxicity in HT22 Hippocampal Neuronal Cells by Upregulating the SIRT1/Wnt/β-Catenin Pathway

Protective Effect of NGR1 against Glutamate-Induced Cytotoxicity in HT22 Hippocampal Neuronal Cells by Upregulating the SIRT1/Wnt/β-Catenin Pathway

Ecdysteroids from the Korean Endemic Species Ajuga spectabilis with Activities against Glucocorticoid Receptors and 11β-Hydroxysteroid Dehydrogenase Type 1

Chemical Constituents of the Leaves of Diospyros kaki (Persimmon)

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