Phenolic contents-based assessment of therapeutic potential of Syzygium cumini leaves extract

Abstract

Syzygium cumini (S . cumini) is an evergreen tropical plant that is well recognized for its therapeutic potential of common diseases. In this study, the therapeutic potential and biomedical application of S . cumini are assessed in vitro and in vivo to find its effectiveness for different complications. The methanolic crude extract of S . cumini leaves were screened for total phenolic and flavonoid content. In vitro , the DPPH scavenging assay, XTT assay, prothrombin and activated partial thromboplastin time were used to assess antioxidant, cytoprotective and thrombolytic activity of the S . cumini extract, respectively. The anti-inflammatory potential and the analgesic activity of the S . cumini extract were analyzed in rabbits by the Carrageenan induced paw edema method and the writhing method, respectively. Phytochemical analysis showed the presence of considerable amounts of total phenolic (369.75 ± 17.9 mg GAE/g) and flavonoid (75.8 ± 5.3 mgRE/g) content in the S . cumini extract. The DPPH assay demonstrated a higher antioxidant potential (IC-50 value of 133 μg/ml), which was comparable to the IC-50 of ascorbic acid (122.4 μg/ml). Moreover, the S . cumini extract showed a dose dependent cytoprotective effect against H 2 O 2 treated bone marrow mesenchymal stem cells (BM-MSCs). S . cumini also possesses significant anticoagulant activity with a prothrombin time of 28.3 ± 1.8 seconds vs 15.8 ± 0.2 seconds of control, p<0.05. The leaf extract also demonstrated an analgesic effect in rabbits as indicated by the decrease in writhing (12.2 ± 1.7 control vs. 3.7 ± 0.6 treated) and anti-inflammatory activity in rabbits paw with a protection against inflammation of 64.1 ± 2.4%. Our findings suggest that the methanolic extract of S . cumini leaves has antioxidant, cytoprotective, anticoagulant, analgesic and anti-inflammatory properties, and therefore, can be applied for treating cardiovascular diseases and cancers.

Formulation and organoleptic evaluation of Poly Herbal Cream of Punica, Neem, Carrot & Jamun as Active Ingredients

“…The pulp and seeds are used for traditional medicine against diabetes, diarrhea, and ringworm infection also protect against radiation-induced sickness 32,[34][35][36][37] . It is very beneficial as it has anti-diabetic, cytoprotective, anticoagulant, analgesic and anti-inflammatory, anti-cancerous, anti-microbial, anti-oxidant, hypo-lipidemic, hepato-protective properties 35,38,39 .…”

Exploration of the Chemical Potential and Antioxidant Activity of Some Plants Used in the Treatment of Male Infertility in Southern Benin

“…The total polyphenol content in the various extracts was calculated from a linear calibration curve (y = ax + b), established with precise gallic acid concentrations as a reference standard (0-200 µg/mL). The total phenolic content was determined as mg of gallic acid equivalent/g of extract (mg GAE/g) by using formula Ahmed et al [14]:…”

“…The quantities of flavonoids in the extracts were calculated from the calibration curve of a standard flavonoid (Rutin) as a reference standard (0-1 mg/mL). The total flavonoids content was determined as µg of rutin equivalent/g of extract (µgRuE/g) by using the formula Ahmed et al [14] TFC = (X x V)/m; where TFC is total flavonoids content, X is the rutin concentration in mg/ml; V is the extract volume used in ml and m is the weight of extract in grams.…”

Physicochemical, ADMET and Druggable properties of Myricetin: A Key Flavonoid in Syzygium cumini that regulates metabolic inflammations

“…[67] CODEN (USA): JDDTAO have been identified to date, including 8000 different types of polyphenols for their therapeutic potential 3 .…”

“…S. cumini has been reported to contain Ellagic acid, Triterpenoids, acetyl Oleanolic acid, Quercetin, Isoquercitin, Myricetin and Kaempferol 20 . S. cumini possesses enormous phytochemicals, of all, Myricetin has been widely reported for hypoglycemic, antimicrobial, hypolipidemic, anti-allergic, antiinflammatory, cardio-protective, hepatoprotective and antineoplastic properties 3,6,20,22 .…”