Phenolic Extract of Seagrass, <i>Halophila ovalis</i> Activates Intrinsic Pathway of Apoptosis in Human Breast Cancer (MCF-7) Cells

Sharma, Harshita; Stephen, Nimish Mol; Gopal, Sowmya Shree; Rudresh, Deepika Udayawara; Kavalappa, Yogendra Prasad; Shivarudrappa, Arpitha Haranahalli; Hithamani, Gavirangappa; Ponesakki, Ganesan

doi:10.1080/01635581.2020.1743874

Cited by 11 publications

(5 citation statements)

References 26 publications

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“…In our study, HUE induced intrinsic apoptosis, as indicated by the activation of caspase-3, the downregulation of the anti-apoptotic Bcl-2 protein, and the upregulation of the pro-apoptotic Bax protein ( Figure 5 ). This is in line with other studies reporting the pro-apoptotic effects of different seagrasses, Syringodium isoetifolium and Halophila ovalis , on the MCF-7 breast cancer cell line [ 61 , 63 ]. This is also similar to results obtained with other plant extracts, including Ziziphus nummularia and Origanum syriacum , on TNBC [ 25 , 77 ].…”

Section: Discussionsupporting

confidence: 92%

“…Previous studies have reported the anti-proliferative effects of H. uninervis extracts on different cancer cell lines [ 18 , 20 ]. It is worth noting that the anti-proliferative effects of different seagrasses, including Syringodium isoetifolium , Cymodocea serrulata , Halophila ovalis , and Halodule pinifolia (a different species of H. uninervis ), on the ER-positive breast cancer subtype are well documented [ 61 , 62 , 63 , 64 ]. However, this is the first study to assess the effects of H. uninervis ethanolic extract (HUE) on the triple-negative subtype of breast cancer.…”

Section: Discussionmentioning

confidence: 99%

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The Antioxidant Potential and Anticancer Activity of Halodule uninervis Ethanolic Extract against Triple-Negative Breast Cancer Cells

Wehbe,

Badran,

Baydoun

et al. 2024

Antioxidants

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Natural remedies have been indispensable to traditional medicine practices for generations, offering therapeutic solutions for various ailments. In modern times, these natural products continue to play a pivotal role in the discovery of new drugs, especially for cancer treatment. The marine ecosystem offers a wide range of plants with potential anticancer activities due to their distinct biochemical diversity and adaptation to extreme situations. The seagrass Halodule uninervis is rich in diverse bioactive metabolites that bestow the plant with various pharmacological properties. However, its anticancer activity against invasive triple-negative breast cancer (TNBC) is still poorly investigated. In the present study, the phytochemical composition of an ethanolic extract of H. uninervis (HUE) was screened, and its antioxidant potential was evaluated. Moreover, the anticancer potential of HUE against MDA-MB-231 cells was investigated along with the possible underlying mechanisms of action. Our results showed that HUE is rich in diverse phytochemicals that are known for their antioxidant and anticancer effects. In MDA-MB-231 cells, HUE targeted the hallmarks of cancer, including cell proliferation, adhesion, migration, invasion, and angiogenesis. The HUE-mediated anti-proliferative and anti-metastatic effects were associated with the downregulation of the proto-oncogenic STAT3 signaling pathway. Taken together, H. uninervis could serve as a valuable source for developing novel drugs targeting TNBC.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

The Antioxidant Potential and Anticancer Activity of Halodule uninervis Ethanolic Extract against Triple-Negative Breast Cancer Cells

Wehbe,

Badran,

Baydoun

et al. 2024

Antioxidants

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“…Doxorubicin was used as a positive control with IC 50 value of 1.2 µg/ml. Natural products rich in kaempferol and caffeic acid induce cytotoxic effect against breast cancer MCF-7 via induction of apoptosis [ 42 , 43 ].…”

Section: Resultsmentioning

confidence: 99%

In-vitro study of cytotoxic and apoptotic potential of Thalassia hemprichii (Ehren.) Asch. And Enhalus acoroides (L.f.) Royle against human breast cancer cell line (MCF-7) with correlation to their chemical profile

Mohamed,

Elsayed,

El Shaffai

et al. 2024

BMC Complement Med Ther

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Background Breast cancer is the most common type of cancer diagnosed in women. Finding novel therapeutic agents with significant cytotoxic action and minimal adverse impact on normal cells becomes crucial. Today, natural anticancer agents present an unconventional method of treating cancer, either as a curative or preventative agent, with considerable concern for marine organisms. Methods The anticancer effect of the alcoholic extract of different Red Sea Seagrasses on MCF-7 human breast cancer cell line has been investigated. Seagrasses were collected from Wadi El Gamal, Red Sea and extracted. Qualitative HPLC analysis was performed on the extracts for the identification of their active biomarkers. This study was aimed to explore the cytotoxic impact of Thalassia hemprichii (Ehren.) and Enhalus acoroides (L.f.) Royle on MCF-7 and their mode of action. Their anti-proliferative effects on cancer cells were performed using Neutral red assay. On the other hand, their apoptotic effect and their capacity to induce cell cycle arrest were investigated by flow cytometry assay. The effect of Seagrasses on the mitochondrial membrane potential (ΔψM) was studied by using JC-1 mitochondrial membrane potential assay kit in Seagrasses treated cancer cells to Δψ Caspases 3/7activity was examined using the colorimetric method. Gene expression analysis and quantitative real time RT-PCR for the sea grasses on MCF-7 was performed. Immune-blotting technique for Bcl-2 and p53 was investigated. Results HPLC analysis demonstrated that the extracts contained mainly flavonoids and polyphenols such as Caffeic acid, Chlorogenic acids, catechin and kaempferol that might be responsible for these anticancer effects. Seagrasses alcoholic crude extract markedly suppressed the growth and expansion of MCF-7 cells concentration-dependently with no toxicity against normal human skin fibroblast HSF. Thalassia hemprichii and Enhalus acoroides trigger mode of cell death primarily via apoptosis as confirmed by the flow cytometry. Additionally, they have ability to induce G0/S cell cycle arrest in MCF-7. The data showed the depletion in mitochondrial membrane potential (ΔψM) in the treated cells dose-dependently Caspases 3/7activities markedly increased following 24 h treatment. Finally, Gene expression analysis showed a marked reduction in Bcl-2, Survivin and CDC2 gene expression levels and a significant increase in the expression of p53 and CC2D1A as compared to control cells. Conclusion In summary, the Methanolic extract of seagrass, Thalassia hemperchii and Enhalus ocoroides are able to induce concentration-dependent cytotoxic effects in human MCF-7 cells through intrinsic pathway of apoptosis in MCF-7 cells. This study reveals the beneficial importance of sea grasses as a source of anticancer agents. Further in vivo study is recommended for the active isolated biomolecules.

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“…In 2019, a study reported that phenolic extracts from Halophila ovalis effectively inhibited the growth of breast cancer cells (MCF-7), indicating potential anti-cancer activity [86] . Furthermore, a previous In vitro study using methanol extracts of this species showed significant anti-bacterial effects as well as noticeable anti-oxidant and antiinflammatory effects [87] . However, the literature lacks comprehensive knowledge on the potential anti-diabetic and anti-microbial effects of Halophila ovalis extracts.…”

Section: Hydrocharitaceae Familymentioning

confidence: 87%

Medicinal properties of Qatari wetland plants: A review

Al-Jaber,

Abu-Rub,

Kunhipurayil

et al. 2024

Int. J. Herb. Med.

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For over 60,000 years, human dependence on herbal remedies has been a cornerstone of traditional medicine. However, recent times have witnessed escalating concerns about the sustainability of these practices, especially regarding their impact on local culture, biodiversity, and environmental integrity. This issue is particularly pronounced in Qatar, where wetlands play a vital role yet remain insufficiently studied. A comprehensive literature review aimed at identifying wetland plant species traditionally used by the people of Qatar revealed 17 such species from six families, predominantly from the Amaranthaceae family. These plants, thriving in diverse habitats, exhibit promising therapeutic properties, including anti-cancer, anti-diabetic, anti-inflammatory, and anti-microbial activities. Noteworthy is that over 50% of the examined plants possess both antioxidant and anti-inflammatory attributes. Despite their potential, less than 30% of these plants have been explored for their anti-cancer potential. Conservation efforts are imperative to safeguard these unique wetland ecosystems, and further research is essential to validate and unlock the full therapeutic potential of these plants for the development of potent, plant-based drugs.

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Phenolic Extract of Seagrass, Halophila ovalis Activates Intrinsic Pathway of Apoptosis in Human Breast Cancer (MCF-7) Cells

Cited by 11 publications

References 26 publications

The Antioxidant Potential and Anticancer Activity of Halodule uninervis Ethanolic Extract against Triple-Negative Breast Cancer Cells

The Antioxidant Potential and Anticancer Activity of Halodule uninervis Ethanolic Extract against Triple-Negative Breast Cancer Cells

In-vitro study of cytotoxic and apoptotic potential of Thalassia hemprichii (Ehren.) Asch. And Enhalus acoroides (L.f.) Royle against human breast cancer cell line (MCF-7) with correlation to their chemical profile

Medicinal properties of Qatari wetland plants: A review

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