Phenolic fractions from nine <i>Trifolium</i> species modulate the coagulant properties of blood plasma <i>in vitro</i> without cytotoxicity towards blood cells

Kołodziejczyk-Czepas, Joanna; Sieradzka, Malgorzata; Moniuszko-Szajwaj, Barbara; Nowak, Paweł; Oleszek, Wiesław; Stochmal, Anna

doi:10.1111/jphp.12872

Cited by 11 publications

(5 citation statements)

References 36 publications

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“…alexandrinum extract showed strong activity towards aPTT even at concentrations as low as 3 mg/ml while no effect on PT was observed. The obtained aPTT activity was opposing to the previously obtained results that suggested that the plant did not affect the coagulation times (TT, PT or aPTT) (Kolodziejczyk-Czepas et al 2018). Difference in the sample preparation could be one of the reasons for the differences in the results as in our study we used the total ethanolic extract in in vitro testing; compared to using phenolic fractions in the mentioned study.…”

Section: Discussioncontrasting

confidence: 84%

Investigation of the effect of selected edible and medicinal plants on in-vitro blood coagulation profile

Fathy

Mahrous²,

El-Khair³

et al. 2021

KJS publishes peer-review articles in Mathematics, Computer Sci

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Anticoagulation therapy represents a mainstay of treatment and prevention of cardiovascular diseases which are the leading causes of mortality worldwide. In addition, several case reports of spontaneous bleeding occurred linking the consumption of many dietary supplements during treatment with anticoagulants or in postoperative patients. This prompted our study which is conducted on eleven well-known Egyptian medicinal plants to highlight their effect on blood coagulation profile using Prothrombin time (PT) and activated partial thromboplastin time (aPTT) tests. Some of these plants showed interesting results that need more in-depth evaluation of their anticoagulant activity as Hibiscus sabdarifa calyx for its effect on PT. In addition, extracts of Trifolium alexandrinum aerial parts and Pimpinella anismum fruit were proved to affect aPTT. Post operative or cardiovascular patients using herbal supplements should be cautioned about food-drug or herb-drug interactions and to adjust their herbal medication regimen before surgery.

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Section: Discussioncontrasting

confidence: 84%

Investigation of the effect of selected edible and medicinal plants on in-vitro blood coagulation profile

Fathy

Mahrous²,

El-Khair³

et al. 2021

KJS publishes peer-review articles in Mathematics, Computer Sci

View full text Add to dashboard Cite

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“…In accordance with those findings reported in literature caffeic acid or its derivatives can be strong candidates for their observed effects on blood coagulation. Our findings were also in accordance with those reported in the literature [25][26][27][28][29][30]. The extracts with relatively high caffeic acid and other phenolic acids contents acted as an anti-coagulant substance.…”

Section: Hplc and Lc-qtof-ms Analysessupporting

confidence: 93%

Evaluation of the hemostatic activity of Equisetum arvense extract: the role of varying phenolic composition and antioxidant activity due to different extraction conditions

2019

Biointerface Res Appl Chem

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Hemostasis is a procedure that causes bleeding to stop and is very important in injuries. Recent researches were focused on the discovery of plant hemostatic agents. Therefore, plant extracts were investigated as a major source as a hemostatic agent. In this research, Equisetum arvense extract was investigated as a candidate coagulatory agent. Effect of extraction parameters on coagulation was investigated. Although it has been stated in the literature that the extract of Equisetum arvense has anticoagulatory activity, it has been shown in this study that alteration of the extraction parameters changes the extract bioactivity from the anticoagulant to the coagulant.

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“…Measurements were performed using a kinetic model, as described in our previous work [ 53 ]. Briefly, blood plasma was preincubated with the examined substances (at final concentrations of 10, 25, and 50 mg/L) for 15 min at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Synthesis and Hemostatic Activity of New Amide Derivatives

et al. 2022

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Eight dipeptides containing antifibrinolytic agents (tranexamic acid, aminocaproic acid, 4-(aminomethyl)benzoic acid, and glycine—natural amino acids) were synthesized in a three-step process with good or very good yields. DMT/NMM/TsO− (4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium toluene-4-sulfonate) was used as a coupling reagent. Hemolysis tests were used to study the effects of the dipeptides on blood components. Blood plasma clotting tests were used to examine their effects on thrombin time (TT), prothrombin time (PT), and the activated partial thromboplastin time (aPTT). The level of hemolysis did not exceed 1%. In clotting tests, TT, PT, and aPTT did not differentiate any of the compounds. The prothrombin times for all amides 1–8 were similar. The obtained results in the presence of amides 1–4 and 8 were slightly lower than for the other compounds and the positive control, and they were similar to the results obtained for TA. In the case of amide 3, a significantly decreased aPTT was observed. The aPTTs observed for plasma treated with amide 3 and TA were comparable. In the case of amide 6 and 8, TT values significantly lower than for the other compounds were found. The clot formation and fibrinolysis (CFF) assay was used to assess the influence of the dipeptides on the blood plasma coagulation cascade and the fibrinolytic efficiency of the blood plasma. In the clot formation and fibrinolysis assay, amides 5 and 7 were among the most active compounds. The cytotoxicity and genotoxicity of the synthesized dipeptides were evaluated on the monocyte/macrophage peripheral blood cell line. The dipeptides did not cause hemolysis at any concentrations. They exhibited no significant cytotoxic effect on SC cells and did not induce significant DNA damage.

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Phenolic fractions from nine Trifolium species modulate the coagulant properties of blood plasma in vitro without cytotoxicity towards blood cells

Abstract: The most efficient anticoagulant activity in plasma was found for T. fragiferum and T. incarnatum extracts, while the T. alexandrinum fraction was the most effective inhibitor of thrombin amidolytic activity.

Cited by 11 publications

References 36 publications

Investigation of the effect of selected edible and medicinal plants on in-vitro blood coagulation profile

Investigation of the effect of selected edible and medicinal plants on in-vitro blood coagulation profile

Evaluation of the hemostatic activity of Equisetum arvense extract: the role of varying phenolic composition and antioxidant activity due to different extraction conditions

Synthesis and Hemostatic Activity of New Amide Derivatives

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