Phenolic Profile and Cholinesterase, Tyrosinase, Urease and Lipid Peroxidation Inhibition Potentials of Artemisia argyi from Ordu, Turkey

Abstract

The present study was designed to reveal a natural resource that could cure for today's major diseases such as Alzheimer, Parkinson and bacterial infection. Furthermore, oxidative stress resulting from lipid peroxidation is a precursor for many diseases. Therefore, anticholinesterase, antityrosinase, antiurease activities and the inhibition potential on lipid peroxidation of methanolic extract of Artemisia argyi collected from Ordu region were tested. The inhibition ratio of extract at 0.3 mg/mL concentration for asetylcholinesterase and butrylcholinesterase were calculated as 8.45 and 10.68%, respectively. IC50 values for tyrosinase and urease inhibition properties were also found as 0.505 mg/mL and 0.225 mg/mL. The all obtained values were compared with known corresponding inhibitors such as galantamine, kojic acid and thiourea. Besides these, 0.1 mg/mL of the extract prevented lipid peroxidation in the ratio of 7.19%. In the scope of this study, the phenolics of the leaves of A. argyi were also analyzed by using HPLC system and pyrogallol was identified as the major component with the 30.16 mg/kg concentration. On the other hand, benzoic acid was not detected in extract. According to obtained results, A. argyi from Ordu, has the proposed characteristics that it can be a natural resource that can be used in the field of medicine for treatment of various diseases especially Alzheimer and Parkinson, in the cosmetic industry for the elimination of skin problems and in the agricultural field in order to prevent the loss of nitrogenous components.

Phenolic compounds profile, neuroprotective effect and antioxidant potential of a commercial Turkish coffee

“…Elution was carried out using 0.1% acetic acid as the solvent A and acetonitrile as the solvent B with a gradient program starting with 100% A, to reach 5% B after 2 min, 40% B after 20 min and 80% B after 30min. Optimization of the operation was performed by adjusting the injection volume of samples and standards to 10μL, flow rate to 0.9 mL/min, column temperature to 25°C [18,19].…”

“…After 10 minutes incubation of well-mixed mixtures, changes in absorbance were measured against methanol at 234nm. Extract-free mixture was used as a blank [19].…”