Phenolic profiles and brine shrimp cytotoxicity of the ethanolic extract from the aerial part of Crocus alatavicus L.

Satybaldiyeva, Dariya; Mursaliyeva, Valentina; Mammadov, Ramazan; Zayadan, B. K.; Biotechnology, Kazakhstan

doi:10.26577/2218-7979-2016-9-1-38-41

Cited by 4 publications

(5 citation statements)

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“…Polyphenols, including flavonoids, are widely distributed plant secondary metabolites with well-documented biological activity, mainly antibacterial, antifungal, antiviral and anticancer properties [ 11 , 12 , 13 ]. The total polyphenol content in the CA extract in the present study was 64.84 ± 0.88 mg GAE (gallic acid equivalent)/g (data not shown) and was comparable to the data presented by Satybaldiyeva et al [ 8 ].…”

Section: Discussionsupporting

confidence: 92%

“…As reported elsewhere [ 8 ], the ethanolic extract from the aerial part of C. alatavicus growing in Kazakhstan was suggested to possess the potential anticancer activity based on data from the brine shrimp assay. In this paper, significant cytotoxic activity of the extract against the LNCaP cell line was found with an IC 50 value of 1.95 mg/mL.…”

Section: Discussionmentioning

confidence: 89%

“…Satybaldiyeva et al [ 8 ] identified nine phenolic acids in the ethanolic extract of the aerial part of C. alatavicus growing in Kazakhstan, namely gallic acid, 3,4-dihydroxybenzoic acid, 4-hydroxy-benzoic acid, chlorogenic acid, vanillic acid, caffeic acid, p-coumaric acid, ferulic acid, cinnamic acid. In the present paper, carboxyvanilic acid, as well as other acids, were detected in the CA extract, e.g., citric, malic and gluconic acids.…”

Section: Discussionmentioning

confidence: 99%

“…However, there are only limited data on Crocus alatavicus Regel & Semen concerning its phytochemistry and biological activity [ 2 , 7 , 8 ]. It should be noted that this endemic and rare crocus, known as alatau or white saffron, is an early spring ephemeral and geophytic-geocarpic plant that grows in mountain meadows in several central Asian countries, including Kazakhstan [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical Profile and Biological Activity of the Ethanolic Extract from the Aerial Part of Crocus alatavicus Regel & Semen Growing Wildly in Southern Kazakhstan

et al. 2022

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The composition of the ethanolic extract from the aerial parts of Crocus alatavicus Regel & Semen from southern Kazakhstan spontaneous flora was analyzed together with the determination of its antibacterial, antifungal, antiviral and anticancer activity. The phytochemical profile analysis by high-performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (HPLC/ESI-QTOF-MS) revealed the presence of multiple kaempferol derivatives. High-performance reverse-phase liquid chromatography combined with a photodiode-array detection (RP-HPLC/PDA) found that kaempferol 3-O-dihexoside and kaempferol 3-O-acyltetrahexoside accounted for 70.5% of the kaempferol derivatives. The minimum inhibitory concentration (MIC) values of the extract for all the tested reference microorganisms were high, reaching 10 mg/mL for yeasts and 20 mg/mL for bacteria. In contrast, antiviral activity was observed at 2 mg/mL, resulting in the inhibition of the HSV-1-induced cytopathic effect and the reduction in virus infectious titer by 1.96 log, as well as the viral load by 0.85 log. Among the tested prostate cancer cell lines, significant cytotoxic activity of the extract was noted only on the LNCaP cell line, with an IC50 value of 1.95 mg/mL. The LNCaP cell line treated with 2 mg/mL of the extract showed a noticeably reduced number of spindle-shaped cells with longer cellular projections, a significant increase in the peak corresponding to the population of apoptotic cells in the sub-G1 phase and a decreased intracellular glutathione (GSH) level, suggesting the prooxidative properties of the extract. The obtained data provide novel information about the flavonoids present in the aerial part of C. alatavicus and suggest its potential application as a source of the compounds active against HSV-1 and metastatic, androgen-sensitive prostate cancer.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phytochemical Profile and Biological Activity of the Ethanolic Extract from the Aerial Part of Crocus alatavicus Regel & Semen Growing Wildly in Southern Kazakhstan

et al. 2022

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“…[15]. The presence of antimicrobial [16,17], antioxidant [17,18] cytotoxic [19], and other activities have been established. These data show the promise of studying and using not only saffron.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Analysis and Antioxidant Activity of Crocus speciosus Leaves

Мykhailenko¹,

Безрук²,

Volochai³

et al. 2022

Phyton

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The numerous studies indicate leaves of plants are a rich source of bioactive compounds that can be a valuable source of compounds used in the pharmaceutical and cosmetic industries. Aim of this study was to investigate the chemical composition and the antioxidant property of Crocus speciosus leaves. Primary phytochemical screening of C. speciosus leaves revealed the presence of some following compound categories such as phenolic compounds, aminoacids, saponins, proteins, tannins, triterpenoids, glycosides, polysaccharides. The total flavonoids and phenolic compounds content were determined spectrophotometrically and by HPLC-DAD and HPLC-MS. Antiradical activity was determined by ABTS radical-cation scavenging method, spectrophotometrically. The total amount of flavonoids in C. speciosus leaves was 1.07 ± 0.02 mg RE/g (p < 0.05), the total amount of phenolic compounds was 0.41 ± 0.01 mg GAE/g (p < 0.05). By HPLC-DAD-MS analysis the presence of the mangiferin, chlorogenic acid, isoorientin, kaempferol, hyperoside, and isoquercitin was established for the first time in Crocus leaves. The antiradical activity of C. speciosus leaves extracts was 150.08 ± 4.5 μmol/g (p < 0.05) and its was mainly attributed to phenolic compounds content. The high amounts of flavonoids and antiradical activity in C. speciosus leaves suggests promising phytochemical and pharmacological study of this Crocus species.

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Biologically active compounds and pharmacological activities of species of the genus Crocus: A review

et al. 2019

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Phenolic profiles and brine shrimp cytotoxicity of the ethanolic extract from the aerial part of Crocus alatavicus L.

Cited by 4 publications

References 0 publications

Phytochemical Profile and Biological Activity of the Ethanolic Extract from the Aerial Part of Crocus alatavicus Regel & Semen Growing Wildly in Southern Kazakhstan

Phytochemical Profile and Biological Activity of the Ethanolic Extract from the Aerial Part of Crocus alatavicus Regel & Semen Growing Wildly in Southern Kazakhstan

Phytochemical Analysis and Antioxidant Activity of Crocus speciosus Leaves

Biologically active compounds and pharmacological activities of species of the genus Crocus: A review

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