Phenolic Profiling of the South American “Baylahuen” Tea (Haplopappus spp., Asteraceae) by HPLC-DAD-ESI-MS

Abstract

Abstract:The aerial parts of several Haplopappus species (Asteraceae), known under the common name "baylahuen", are used as herbal teas in Chile and Argentina. In Chile, "baylahuen" comprises H. multifolius, H. taeda, H. baylahuen and H. rigidus. Little is known about the chemical identity of the infusion constituents in spite of widespread consumption. The aim of the present work was the characterization of phenolics occurring in the infusions and methanol extracts of "baylahuen" by HPLC-DAD-ESI-MS. A simple HPLC-DAD-ESI-MS method was developed for the fast identification and differentiation of Haplopappus spp. used as a tea source, based on the phenolics from the tea and methanol extracts. Some 27 phenolics were tentatively identified in the infusions and methanol extract, including 10 caffeoyl quinic and feruloyl quinic acid derivatives and 17 flavonoids. The HPLC patterns of the Haplopappus tea and methanol extract allow a clear differentiation at the species level. The occurrence of hydroxycinnamic acid derivatives and flavonoids can explain the reputed nutraceutical and health beneficial properties of this herbal tea.

Comprehensive Chemical Profiling in the Ethanol Extract of Pluchea indica Aerial Parts by Liquid Chromatography/Mass Spectrometry Analysis of Its Silica Gel Column Chromatography Fractions

“…Quinic acids and their derivatives are one kind of the most characteristic components in Asteraceae family plants [19,20,21]. There are four hydroxyl groups substituted at 1-, 3-, 4-, and 5-position, as well as one carboxyl at 1-position of quinic acid, respectively, which means that the quinic acids derivatives can be formed at any one of above mentioned position or functional group theoretically.…”

Absolute Configuration of 18-Acetoxy-Cis-Cleroda-3,13e-Dien-15-Oic Acid

“…In the crystal the molecules are linked by one intermolecular OH⋅⋅⋅O hydrogen bond forming 1D-dimensional chain with graph-set notation (15) 23 (O2H2⋅⋅⋅O4 i 149°; O2⋅⋅⋅O4 i 2.797(9) Å; H2⋅⋅⋅O4 i 2.06 Å (symmetry code (i) x,-1+y,1+z), (Figure 4). The absolute configuration of the title compound which has been determined from the refinement of the Flack parameter 19 , x = 0.0 (5), which indicate that the correct configuration had been assigned against 1353 (99%) CuKα Bijvoet pairs. On this basis the absolute configuration was assigned as C5R, C8S, C9R and C10S.…”

“…All the related Chilean Haplopappus "baylahuen" species produce a resinous exudate with interesting biologically active compounds including, several clerodane and labdane diterpenoids and flavonoids with biological significance 2 . Indeed, the Mapuche medicinal plants Haplopappus multifolius, H. taeda, H. baylahuen and remyanus showed the presence of flavonoids and labdane terpenoids with known antioxidant, choleretic, anti-inflammatory and cholagogue properties 3,4,5 . In the Aymara region, the infusion of H. rigidus has been used to cure or prevent liver diseases, gastro-intestinal disorders, cough and has also been employed as a sexual stimulant attributed to the presence of diterpenoids and flavonoids 1,6,7 .…”

Phytochemical screening and biological assays of ethanolic leaf extract of Senna rugosa

“…As no chromatographic separation was performed in the HPLC, the chemical profile of the fractions rich in acidic compounds (8 th e 9 th ) was achieved by direct infusion in the ESI-MS (Shimadzu ® LCMS-2020, Kyoto, Japan) set to negative mode. The identities of the main peaks in the phenolic fraction were suggested based on information reported in references (Gill & Morgan 2001;Bastos et al 2007;Sang et al 2007;Falcão et al 2010;Navarre et al 2011;Hamed et al 2014;Spindola et al 2014;Schmeda-Hirschmann et al 2015;El Sayed et al 2016).…”